Recruitment kinetics of the homologous recombination pathway in procyclic forms of Trypanosoma brucei after ionizing radiation treatment

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dc.contributorLaboratório Especial de Ciclo Celularpt_BR
dc.contributor.authorMarin, Paula Andreapt_BR
dc.contributor.authorSilva, Marcelo Santos dapt_BR
dc.contributor.authorPavani, Raphael Souzapt_BR
dc.contributor.authorMachado, Carlos Renatopt_BR
dc.contributor.authorElias, Maria Carolinapt_BR
dc.date.accessioned2020-07-09T21:19:08Z-
dc.date.available2020-07-09T21:19:08Z-
dc.date.issued2018-
dc.identifier.citationMarin PA, Silva MS, Pavani RS, Machado CR, Elias MC. Recruitment kinetics of the homologous recombination pathway in procyclic forms of Trypanosoma brucei after ionizing radiation treatment. Sci Rep. 2018 Mar;8:5405. doi:10.1038/s41598-018-23731-6.pt_BR
dc.identifier.issn2045-2322-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2399-
dc.description.abstractOne of the most important mechanisms for repairing double-strand breaks (DSBs) in model eukaryotes is homologous recombination (HR). Although the genes involved in HR have been found in Trypanosoma brucei and studies have identified some of the proteins that participate in this HR pathway, the recruitment kinetics of the HR machinery onto DNA during DSB repair have not been clearly elucidated in this organism. Using immunofluorescence, protein DNA-bound assays, and DNA content analysis, we established the recruitment kinetics of the HR pathway in response to the DSBs generated by ionizing radiation (IR) in procyclic forms of T. brucei. These kinetics involved the phosphorylation of histone H2A and the sequential recruitment of the essential HR players Exo1, RPA, and Rad51. The process of DSB repair took approximately 5.5 hours. We found that DSBs led to a decline in the G2/M phase after IR treatment, concomitant with cell cycle arrest in the G1/S phase. This finding suggests that HR repairs DSBs faster than the other possible DSB repair processes that act during the G1/S transition. Taken together, these data suggest that the interplay between DNA damage detection and HR machinery recruitment is finely coordinated, allowing these parasites to repair DNA rapidly after DSBs during the late S/G2 proficient phases.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent5405pt_BR
dc.languageengpt_BR
dc.publisherNature Publishing Grouppt_BR
dc.relation.ispartofScientific Reportspt_BR
dc.rightsOpen Accesspt_BR
dc.titleRecruitment kinetics of the homologous recombination pathway in procyclic forms of Trypanosoma brucei after ionizing radiation treatmentpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1038/s41598-018-23731-6pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1038/s41598-018-23731-6pt_BR
dc.contributor.externalUniversidade Federal de Minas Gerais (UFMG)¦¦Brasilpt_BR
dc.publisher.cityLondonpt_BR
dc.identifier.citationvolume8pt_BR
dc.relation.ispartofabbreviatedSci Reppt_BR
dc.identifier.citationabntv. 8, 5405, mar. 2018pt_BR
dc.identifier.citationvancouver2018 Mar;8:5405pt_BR
dc.publisher.countryEnglandpt_BR
dc.contributor.butantanSilva, Marcelo Santos da|:Aluno|:Laboratório Especial de Ciclo Celular|:pt_BR
dc.contributor.butantanPavani, Raphael Souza|:Aluno|:Laboratório Especial de Ciclo Celular|:pt_BR
dc.contributor.butantanElias, Maria Carolina|:Pesquisador|:Laboratório Especial de Ciclo Celular|:Autor de correspondênciapt_BR
dc.contributor.butantanMarin, Paula Andrea|:Aluno|:Laboratório Especial de Ciclo Celular|:PrimeiroAutorpt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦870219/1997-9pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦304329/2015-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/24170-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/10580-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/50050-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.openairetypeArticle-
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item.grantfulltextembargo_29990101-
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