PhcrTx2, a new crab-paralyzing peptide toxin from the sea anemone phymanthus crucifer

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dc.contributorLab. Genéticapt_BR
dc.contributor.authorRodriguez, Armando Alexeipt_BR
dc.contributor.authorGarateix, Anolandpt_BR
dc.contributor.authorSalceda, Emiliopt_BR
dc.contributor.authorPeigneur, Stevept_BR
dc.contributor.authorZaharenko, Andre Junqueirapt_BR
dc.contributor.authorPons, Tirsopt_BR
dc.contributor.authorSantos, Yulicapt_BR
dc.contributor.authorArreguin, Robertopt_BR
dc.contributor.authorStaendker, Ludgerpt_BR
dc.contributor.authorForssmann, Wolf-Georgpt_BR
dc.contributor.authorTytgat, Janpt_BR
dc.contributor.authorVega, Rosariopt_BR
dc.contributor.authorSoto, Enriquept_BR
dc.date.accessioned2020-07-09T21:19:30Z-
dc.date.available2020-07-09T21:19:30Z-
dc.date.issued2018pt_BR
dc.identifier.citationRodriguez AA, Garateix A, Salceda E, Peigneur S, Zaharenko AJ, Pons T, et al. PhcrTx2, a new crab-paralyzing peptide toxin from the sea anemone phymanthus crucifer. Toxins. 2018 Feb;10(2):72. doi:10.3390/toxins10020072.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2423-
dc.description.abstractSea anemones produce proteinaceous toxins for predation and defense, including peptide toxins that act on a large variety of ion channels of pharmacological and biomedical interest. Phymanthus crucifer is commonly found in the Caribbean Sea; however, the chemical structure and biological activity of its toxins remain unknown, with the exception of PhcrTx1, an acid-sensing ion channel (ASIC) inhibitor. Therefore, in the present work, we focused on the isolation and characterization of new P. crucifer toxins by chromatographic fractionation, followed by a toxicity screening on crabs, an evaluation of ion channels, and sequence analysis. Five groups of toxic chromatographic fractions were found, and a new paralyzing toxin was purified and named PhcrTx2. The toxin inhibited glutamate-gated currents in snail neurons (maximum inhibition of 35%, IC50 4.7 mu M), and displayed little or no influence on voltage-sensitive sodium/potassium channels in snail and rat dorsal root ganglion (DRG) neurons, nor on a variety of cloned voltage-gated ion channels. The toxin sequence was fully elucidated by Edman degradation. PhcrTx2 is a new -defensin-fold peptide that shares a sequence similarity to type 3 potassium channels toxins. However, its low activity on the evaluated ion channels suggests that its molecular target remains unknown. PhcrTx2 is the first known paralyzing toxin in the family Phymanthidae.pt_BR
dc.description.sponsorshipFundação Alexander von Humboldtpt_BR
dc.description.sponsorshipGerman Research Foundation (DFG)pt_BR
dc.description.sponsorshipInternational Foundation for Science (IFS)pt_BR
dc.description.sponsorshipOrganização Europeia de Biologia Molecular (EMBO)pt_BR
dc.description.sponsorshipAcademia Mundial de Ciências (TWAS) Itáliapt_BR
dc.description.sponsorshipGovernment of Veracruz (Mexico)pt_BR
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología (CONACYT)pt_BR
dc.description.sponsorshipVIEP - Benemérita Universidad Autónoma de Pueblapt_BR
dc.description.sponsorshipPrograma de Fortalecimiento de la Calidad en Instituciones Educativas (PROFOCIE)pt_BR
dc.description.sponsorshipPrograma Iberoamericano de Ciencia y Tecnología (CYTED)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFonds Wetenschappelijk Onderzoek - Vlaanderenpt_BR
dc.description.sponsorshipInteruniversity Attraction Poles (IAP) Programme of the Belgian Science Policy Officept_BR
dc.description.sponsorshipUniversidade Católica de Leuvenpt_BR
dc.format.extent72pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen Accesspt_BR
dc.titlePhcrTx2, a new crab-paralyzing peptide toxin from the sea anemone phymanthus cruciferpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3390/toxins10020072pt_BR
dc.identifier.urlhttp://dx.doi.org/10.3390/toxins10020072pt_BR
dc.contributor.externalUniversidade de Ulm¦¦Alemanhapt_BR
dc.contributor.externalHannover Medical School (MHH)¦¦Alemanhapt_BR
dc.contributor.externalBenemérita Universidad Autónoma de Puebla (BUAP)¦¦Méxicopt_BR
dc.contributor.externalUniversidade Católica de Leuven¦¦Bélgicapt_BR
dc.contributor.externalConsejo Superior de Investigaciones Cientificas (CSIC)¦¦Espanhapt_BR
dc.contributor.externalUniversity of Florida (UF)¦¦Estados Unidospt_BR
dc.contributor.externalUniversidad Nacional Autónoma de México (UNAM)¦¦Méxicopt_BR
dc.contributor.externalCentro de Bioproductos Marinos (CEBIMAR)¦¦Cubapt_BR
dc.identifier.citationvolume10pt_BR
dc.identifier.citationissue2pt_BR
dc.subject.keywordsea anemonept_BR
dc.subject.keywordneutoxin; glutamate receptorpt_BR
dc.subject.keyworddefensin-like foldpt_BR
dc.subject.keywordion channelspt_BR
dc.subject.keywordPhymanthus cruciferpt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 10, n. 2, 72, fev. 2018pt_BR
dc.identifier.citationvancouver2018 Feb;10(2):72pt_BR
dc.contributor.butantanZaharenko, Andre Junqueira|:Aluno|:Lab. Genética|:pt_BR
dc.sponsorship.butantanAcademia Mundial de Ciências (TWAS)¦¦06344-2007¦¦ Itáliapt_BR
dc.sponsorship.butantanConsejo Nacional de Ciencia y Tecnología (CONACYT)¦¦169835pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFonds Wetenschappelijk Onderzoek - Vlaanderen¦¦G.0433.12pt_BR
dc.sponsorship.butantanFonds Wetenschappelijk Onderzoek - Vlaanderen¦¦GOE3414Npt_BR
dc.sponsorship.butantanFundação Alexander von Humboldt¦¦3.2-KUB/1153731 STPpt_BR
dc.sponsorship.butantanGerman Research Foundation (DFG)¦¦pt_BR
dc.sponsorship.butantanGovernment of Veracruz (Mexico)¦¦pt_BR
dc.sponsorship.butantanInternational Foundation for Science (IFS)¦¦F/4082-1pt_BR
dc.sponsorship.butantanInternational Foundation for Science (IFS)¦¦F/4082-2pt_BR
dc.sponsorship.butantanInteruniversity Attraction Poles (IAP) Programme of the Belgian Science Policy Office¦¦IUAP 7/10pt_BR
dc.sponsorship.butantanOrganização Europeia de Biologia Molecular (EMBO)¦¦167-2007pt_BR
dc.sponsorship.butantanOrganização Europeia de Biologia Molecular (EMBO)¦¦126-2009pt_BR
dc.sponsorship.butantanPrograma de Fortalecimiento de la Calidad en Instituciones Educativas (PROFOCIE)¦¦pt_BR
dc.sponsorship.butantanPrograma Iberoamericano de Ciencia y Tecnología (CYTED)¦¦212RT0467pt_BR
dc.sponsorship.butantanUniversidade Católica de Leuven¦¦OT/12/081pt_BR
dc.sponsorship.butantanVIEP - Benemérita Universidad Autónoma de Puebla¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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