Binding of human plasminogen by the lipoprotein LipL46 of Leptospira interrogans

Full metadata record
DC FieldValueLanguage
dc.contributorLaboratório de Desenvolvimento de Vacinaspt_BR
dc.contributor.authorSantos, Jadson Vianapt_BR
dc.contributor.authorPereira, Priscila Romero Mazzinipt_BR
dc.contributor.authorFernandes, Luis Guilherme Vírgíliopt_BR
dc.contributor.authorSiqueira, Gabriela Hasept_BR
dc.contributor.authorSouza, Gisele O. dept_BR
dc.contributor.authorSouza Filho, Antôniopt_BR
dc.contributor.authorVasconcellos, Silvio A.pt_BR
dc.contributor.authorHeinemann, Marcos B.pt_BR
dc.contributor.authorChapola, Erica G. B.pt_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Oller dopt_BR
dc.date.accessioned2020-07-09T21:19:33Z-
dc.date.available2020-07-09T21:19:33Z-
dc.date.issued2018pt_BR
dc.identifier.citationSantos JV, Pereira PRM, Fernandes LGV, Siqueira GH, Souza GO., Souza Filho A, et al. Binding of human plasminogen by the lipoprotein LipL46 of Leptospira interrogans. Mol. Cell. Probes. 2018 Feb;37:12-21. doi:10.1016/j.mcp.2017.10.004.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2427-
dc.description.abstractLeptospirosis is a widespread zoonosis caused by pathogenic Leptospira. Bacteria disseminate via the bloodstream and colonize the renal tubules of reservoir hosts. Leptospiral surface-exposed proteins are important targets, because due to their location they can elicit immune response and mediate adhesion and invasion processes. LipL46 has been previously reported to be located at the leptospiral outer membrane and recognized by antibodies present in serum of infected hamsters. In this study, we have confirmed the cellular location of this protein by immunofluorescence and FACS. We have cloned and expressed the recombinant protein LipL46 in its soluble form. LipL46 was recognized by confirmed leptospirosis human serum, suggesting its expression during infection. Binding screening of LipL46 with extracellular matrix (ECM) and plasma components showed that this protein interacts with plasminogen. The binding is dose-dependent on protein concentration, but saturation was not reached with the range of protein concentration used. Kringle domains of plasminogen and lysine residues of the recombinant protein are involved in the binding because the lysine analog, amino caproic acid (ACA) almost totally inhibited the reaction. The interaction of LipL46 with plasminogen generates plasmin in the presence of plasminogen activator uPA. Because plasmin generated at the leptospiral surface can degrade ECM molecules and decrease opsonophagocytosis, we tentatively infer that Lip46 has a role in helping the invasion process of pathogenic Leptospira.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extentp. 12-21pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofMolecular and Cellular Probespt_BR
dc.titleBinding of human plasminogen by the lipoprotein LipL46 of Leptospira interroganspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.mcp.2017.10.004pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.mcp.2017.10.004pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.contributor.external(CCZ) Centro de Controle de Zoonoses de São Paulopt_BR
dc.identifier.citationvolume37pt_BR
dc.relation.ispartofabbreviatedMol Cell Probespt_BR
dc.identifier.citationabntv. 37, p. 12-21, fev. 2018pt_BR
dc.identifier.citationvancouver2018 Feb;37:12-21pt_BR
dc.contributor.butantanPereira, Priscila Romero Mazzini|:Aluno|:Laboratório de Desenvolvimento de Vacinas|:pt_BR
dc.contributor.butantanFernandes, Luis Guilherme Virgílio|:Aluno|:Laboratório de Desenvolvimento de Vacinas|:pt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:Laboratório de Desenvolvimento de Vacinas|:Autor de correspondênciapt_BR
dc.contributor.butantanSantos, Jadson Viana|:Aluno|:Laboratório de Desenvolvimento de Vacinas|:PrimeiroAutorpt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦302758/2013-5pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦441449/2014-0pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦125721/2015-3pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦14/50981-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/02590-5pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2012/24164-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-4851-0870-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

Existing users please Login
10.1016j.mcp.2017.10.004.pdf
Size: 967.63 kB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.