Leptospira interrogans outer membrane protein LipL21 is a potent inhibitor of neutrophil myeloperoxidase

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dc.contributorLaboratório de Desenvolvimento de Vacinaspt_BR
dc.contributorCentro de Biotecnologiapt_BR
dc.contributorLab. Imunoquímicapt_BR
dc.contributor.authorVieira, Mônica Laruccipt_BR
dc.contributor.authorTeixeira, Aline Rodrigues Florênciopt_BR
dc.contributor.authorPidde-Queiroz, Gisellept_BR
dc.contributor.authorChing, Ana Tung Chingpt_BR
dc.contributor.authorTambourgi, Denise Vilarinhopt_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Oller dopt_BR
dc.contributor.authorHerwald, Heikopt_BR
dc.date.accessioned2020-07-09T21:19:41Z-
dc.date.available2020-07-09T21:19:41Z-
dc.date.issued2018pt_BR
dc.identifier.citationVieira ML, Teixeira ARF, Pidde-Queiroz G, Ching ATC, Tambourgi DV, Nascimento ALTO, et al. Leptospira interrogans outer membrane protein LipL21 is a potent inhibitor of neutrophil myeloperoxidase. Virulence. 2018 Mar;9(1):414-25. doi:10.1080/21505594.2017.1407484.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2438-
dc.description.abstractLeptospirosis is a widespread zoonotic and neglected infectious disease of human and veterinary concern that is caused by pathogenic Leptospira species. After entrance in the host, pathogenic leptospires evade the host natural defense mechanisms in order to propagate and disseminate to multiple organs. Myeloperoxidase is an enzyme stored in neutrophils azurophilic granules, and is released upon neutrophil activation to produce mainly hypochlorous acid, a strong oxidant and potent antimicrobial agent. In the present investigation, we studied the modulation of myeloperoxidase activity by L. interrogans serovar Copenhageni. We show that leptospires and their culture supernatants are able to inhibit both peroxidase and chlorination activities of myeloperoxidase, without interfering with neutrophil degranulation. By leptospiral outer membrane protein extraction and fractionation, we identified the proteins LipL21 and LipL45 as myeloperoxidase inhibitors, constituting new Leptospira virulence factors. Accordingly, we propose a function for the protein LipL21, one of the most expressed leptospiral outer membrane proteins. Our results show a novel innate immune evasion mechanism by which leptospires interfere with the host response in order to cope with the host oxidative stress and efficiently achieve dissemination and colonization.pt_BR
dc.description.sponsorshipSwedish Foundation for Strategic Research (SSF)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipRagnar Söderberg Foundationpt_BR
dc.description.sponsorshipAlfred Österlunds Stiftelsept_BR
dc.description.sponsorshipKnut and Alice Wallenberg Foundationpt_BR
dc.description.sponsorshipFaculty of Medicine, Lund Universitypt_BR
dc.description.sponsorshipSwedish Research Councilpt_BR
dc.format.extentp. 414-425pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofVirulencept_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/pt_BR
dc.titleLeptospira interrogans outer membrane protein LipL21 is a potent inhibitor of neutrophil myeloperoxidasept_BR
dc.typeArticlept_BR
dc.rights.licenseCC BY-NC-NDpt_BR
dc.identifier.doi10.1080/21505594.2017.1407484pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1080/21505594.2017.1407484pt_BR
dc.contributor.externalLunds Universitet¦¦Suéciapt_BR
dc.identifier.citationvolume9pt_BR
dc.identifier.citationissue1pt_BR
dc.subject.keywordLeptospirosispt_BR
dc.subject.keywordLeptospirapt_BR
dc.subject.keywordhost-pathogen interactionspt_BR
dc.subject.keywordimmune evasionpt_BR
dc.subject.keywordmyeloperoxidasept_BR
dc.subject.keywordneutrophilspt_BR
dc.subject.keywordinnate immunitypt_BR
dc.relation.ispartofabbreviatedVirulencept_BR
dc.identifier.citationabntv. 9, n. 1, p. 414-425, mar. 2018pt_BR
dc.identifier.citationvancouver2018 Mar;9(1):414-25pt_BR
dc.contributor.butantanPidde-Queiroz, Giselle|:Pesquisador|:Lab. Imunoquímica|:pt_BR
dc.contributor.butantanChing, Ana Tung Ching|:Aluno|:Lab. Imunoquímica|:pt_BR
dc.contributor.butantanVieira, Mônica Larucci|:Aluno|:Laboratório de Desenvolvimento de Vacinas|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanTeixeira, Aline Rodrigues Florêncio|:Aluno|:Laboratório de Desenvolvimento de Vacinas:Centro de Biotecnologia|:pt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:Laboratório de Desenvolvimento de Vacinas:Centro de Biotecnologia|:pt_BR
dc.contributor.butantanTambourgi, Denise Vilarinho|:Pesquisador:Docente Permanente PPGTOX|:Lab. Imunoquímica|:pt_BR
dc.sponsorship.butantanAlfred Österlunds Stiftelse¦¦pt_BR
dc.sponsorship.butantanFaculty of Medicine, Lund University¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/18337-4pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/50981-0pt_BR
dc.sponsorship.butantanKnut and Alice Wallenberg Foundation¦¦KAW 2011.0037pt_BR
dc.sponsorship.butantanRagnar Söderberg Foundation¦¦pt_BR
dc.sponsorship.butantanSwedish Foundation for Strategic Research (SSF)¦¦K2014-56X-13413-15-3pt_BR
dc.sponsorship.butantanSwedish Research Council¦¦2013-2438pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
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