Proteomic endorsed transcriptomic profiles of venom glands from Tityus obscurus and T. serrulatus scorpions

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dc.contributor(LETA) Lab. Toxinologia Aplicadapt_BR
dc.contributorLab. Farmacologiapt_BR
dc.contributorLab. Artrópodespt_BR
dc.contributor.authorOliveira, Ursula Castro dept_BR
dc.contributor.authorNishiyama Junior, Milton Yutakapt_BR
dc.contributor.authorSantos, Maria Beatriz Viana dospt_BR
dc.contributor.authorSilva, Andria de Paula Santos dapt_BR
dc.contributor.authorChalkidis, Hipocrates de Menezespt_BR
dc.contributor.authorSouza-Imberg, Andreiapt_BR
dc.contributor.authorCandido, Denise Mariapt_BR
dc.contributor.authorYamanouye, Normapt_BR
dc.contributor.authorDorce, Valquiria Abrão Coronadopt_BR
dc.contributor.authorJunqueira-de-Azevedo, Inácio de Loiola Meirellespt_BR
dc.identifier.citationOliveira UC, Nishiyama Junior MY, Santos MBV, Silva APS, Chalkidis HM, Souza-Imberg A, et al. Proteomic endorsed transcriptomic profiles of venom glands from Tityus obscurus and T. serrulatus scorpions. PLoS One. 2018 Mar;13(3):e0193739. doi:10.1371/journal.pone.0193739.pt_BR
dc.description.abstractBackground Except for the northern region, where the Amazonian black scorpion, T. obscurus, represents the predominant and most medically relevant scorpion species, Tityus serrulatus, the Brazilian yellow scorpion, is widely distributed throughout Brazil, causing most envenoming and fatalities due to scorpion sting. In order to evaluate and compare the diversity of venom components of Tityus obscurus and T. serrulatus, we performed a transcriptomic investigation of the telsons (venom glands) corroborated by a shotgun proteomic analysis of the venom from the two species. Results The putative venom components represented 11.4% and 16.7% of the total gene expression for T. obscurus and T. serrulatus, respectively. Transcriptome and proteome data revealed high abundance of metalloproteinases sequences followed by sodium and potassium channel toxins, making the toxin core of the venom. The phylogenetic analysis of metalloproteinases from T. obscurus and T. serrulatus suggested an intraspecific gene expansion, as we previously observed for T. bahiensis, indicating that this enzyme may be under evolutionary pressure for diversification. We also identified several putative venom components such as anionic peptides, antimicrobial peptides, bradykinin-potentiating peptide, cysteine rich protein, serine proteinases, cathepsins, angiotensin-converting enzyme, endothelin-converting enzyme and chymotrypsin like protein, proteinases inhibitors, phospholipases and hyaluronidases. Conclusion The present work shows that the venom composition of these two allopatric species of Tityus are considerably similar in terms of the major classes of proteins produced and secreted, although their individual toxin sequences are considerably divergent. These differences at amino acid level may reflect in different epitopes for the same protein classes in each species, explaining the basis for the poor recognition of T. obscurus venom by the antiserum raised against other species.pt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.relation.ispartofPloS Onept_BR
dc.rightsOpen accesspt_BR
dc.titleProteomic endorsed transcriptomic profiles of venom glands from Tityus obscurus and T. serrulatus scorpionspt_BR
dc.rights.licenseCC BYpt_BR
dc.contributor.external(FIT) Faculdades Integradas do Tapajóspt_BR
dc.relation.ispartofabbreviatedPLoS Onept_BR
dc.identifier.citationabntv. 13, n. 3, e0193739, mar. 2018pt_BR
dc.identifier.citationvancouver2018 Mar;13(3):e0193739pt_BR
dc.contributor.butantanNishiyama Junior, Milton Yutaka|:Pesquisador|:(LETA) Lab. Toxinologia Aplicada|:pt_BR
dc.contributor.butantanSantos, Maria Beatriz Viana dos|:Aluno|:Lab. Farmacologia|:pt_BR
dc.contributor.butantanSilva, Andria de Paula Santos da|:Aluno|:Lab. Farmacologia|:pt_BR
dc.contributor.butantanSouza-Imberg, Andreia|:Técnico|:Lab. Farmacologia|:pt_BR
dc.contributor.butantanCandido, Denise Maria|:Técnico|:Lab. Artrópodes|:pt_BR
dc.contributor.butantanYamanouye, Norma|:Pesquisador|:Lab. Farmacologia|:pt_BR
dc.contributor.butantanDorce, Valquiria Abrão Coronado|:Pesquisador|:Lab. Farmacologia|:pt_BR
dc.contributor.butantanOliveira, Ursula Castro de|:Aluno|:(LETA) Lab. Toxinologia Aplicada|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanJunqueira-de-Azevedo, Inácio de Loiola Meirelles|:Pesquisador:Docente Permanente PPGTOX|:(LETA) Lab. Toxinologia Aplicada|:pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦Auxpe-Toxinologia 1207/2011pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1pt_BR
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