Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells

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dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorRibeiro, Kleber Silvapt_BR
dc.contributor.authorVasconcellos, Camilla Ioshidapt_BR
dc.contributor.authorSoares, Rodrigo Pedropt_BR
dc.contributor.authorMendes, Maria Tayspt_BR
dc.contributor.authorEllis, Cameron C.pt_BR
dc.contributor.authorAguilera-Flores, Marcelapt_BR
dc.contributor.authorde Almeida, Igor Correiapt_BR
dc.contributor.authorSchenkman, Sergiopt_BR
dc.contributor.authorIwai, Leo Keipt_BR
dc.contributor.authorTorrecilhas, Ana Claudiapt_BR
dc.date.accessioned2020-07-09T21:19:51Z-
dc.date.available2020-07-09T21:19:51Z-
dc.date.issued2018pt_BR
dc.identifier.citationRibeiro KS, Vasconcellos CI, Soares RP, Mendes MT, Ellis CC., Aguilera-Flores M, et al. Proteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cells. J Extracell Vesicles. 2018;7(1):1463779. doi:10.1080/20013078.2018.1463779.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2451-
dc.description.abstractTrypanosoma cruzi, the aetiologic agent of Chagas disease, releases vesicles containing a wide range of surface molecules known to affect the host immunological responses and the cellular infectivity. Here, we compared the secretome of two distinct strains (Y and YuYu) of T. cruzi, which were previously shown to differentially modulate host innate and acquired immune responses. Tissue culture-derived trypomastigotes of both strains secreted extracellular vesicles (EVs), as demonstrated by electron scanning microscopy. EVs were purified by exclusion chromatography or ultracentrifugation and quantitated using nanoparticle tracking analysis. Trypomastigotes from YuYu strain released higher number of EVs than those from Y strain, enriched with virulence factors trans-sialidase (TS) and cruzipain. Proteomic analysis confirmed the increased abundance of proteins coded by the TS gene family, mucin-like glycoproteins, and some typical exosomal proteins in the YuYu strain, which also showed considerable differences between purified EVs and vesicle-free fraction as compared to the Y strain. To evaluate whether such differences were related to parasite infectivity, J774 macrophages and LLC-MK2 kidney cells were preincubated with purified EVs from both strains and then infected with Y strain trypomastigotes. EVs released by YuYu strain caused a lower infection but higher intracellular proliferation in J774 macrophages than EVs from Y strain. In contrast, YuYu strain-derived EVs caused higher infection of LLC-MK2 cells than Y strain-derived EVs. In conclusion, quantitative and qualitative differences in EVs and secreted proteins from different T. cruzi strains may correlate with infectivity/virulence during the host-parasite interaction.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)pt_BR
dc.description.sponsorshipCiências sem Fronteiraspt_BR
dc.description.sponsorshipNational Institute of General Medical Sciences (NIGMS)pt_BR
dc.format.extent1463779pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Extracellular Vesiclespt_BR
dc.rightsOpen Accesspt_BR
dc.titleProteomic analysis reveals different composition of extracellular vesicles released by two Trypanosoma cruzi strains associated with their distinct interaction with host cellspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1080/20013078.2018.1463779pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1080/20013078.2018.1463779pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.contributor.externalFundação Oswaldo Cruz (Fiocruz)¦¦Brasilpt_BR
dc.contributor.externalUniversity of Texas at El Paso (UTEP)¦¦Estados Unidospt_BR
dc.identifier.citationvolume7pt_BR
dc.identifier.citationissue1pt_BR
dc.subject.keywordExtracellular vesiclespt_BR
dc.subject.keywordT. cruzi host interactionpt_BR
dc.relation.ispartofabbreviatedJ Extracell Vesiclespt_BR
dc.identifier.citationabntv. 7, n. 1, 1463779, 2018pt_BR
dc.identifier.citationvancouver2018;7(1):1463779pt_BR
dc.contributor.butantanIwai, Leo Kei|:Pesquisador:Docente Colaborador PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.sponsorship.butantanCiências sem Fronteiras¦¦pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦305065/2016-5pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦PPM-00102-16pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016-01917-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanNational Institute of General Medical Sciences (NIGMS)¦¦2G12MD007592pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
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item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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