Intracellular Delivery of HCV NS3p gene using vectored particles

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dc.contributorLab. Biotecnologia Viralpt_BR
dc.contributor.authorLemos, Marcos Alexandre Nobrept_BR
dc.contributor.authorSuarez-Patino, Sandra Fernandapt_BR
dc.contributor.authorBernardino, Thaissa Consonipt_BR
dc.contributor.authorCoroadinha, Ana Sofiapt_BR
dc.contributor.authorSoares, Hugopt_BR
dc.contributor.authorAstray, Renato Mancinipt_BR
dc.contributor.authorPereira, Carlos Augustopt_BR
dc.contributor.authorJorge, Soraia Attie Calilpt_BR
dc.date.accessioned2020-07-09T21:19:52Z-
dc.date.available2020-07-09T21:19:52Z-
dc.date.issued2018pt_BR
dc.identifier.citationLemos MAN, Suarez-Patino SF, Bernardino TC, Coroadinha AS, Soares H, Astray RM, et al. Intracellular Delivery of HCV NS3p gene using vectored particles. J. Biotechnol.. 2018 May;274:33-9. doi:10.1016/j.jbiotec.2018.03.010.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2452-
dc.description.abstractViral hepatitis caused by the hepatitis C virus (HCV) affects millions of people worldwide. The non-structural protein 3 (NS3), one of the most conserved proteins in HCV, is the target of many therapeutic studies. The NS3 protease domain (NS3p) has a range of cytotoxic T lymphocyte (CTL) epitopes, and synthesizing the protein inside the cells is the most appropriate way to present it to the immune system. We developed a tool to study this kind of presentation, using two vectored particle (VP) systems, one based on the Semliki Forest virus (SFV) and the other on HCV pseudoparticles (HCVpp), both carrying the protease domain of the NS3 gene. In addition to producing the particles, we developed a method to quantify these VPs using qRT-PCR. We produced batches of approximately 2.4x10(4) SFV-NS3p/mu L and 4.0x10(2) HCVpp-NS3p/mu L. BHK-21 and HuH-7 cells treated with the VPs expressed the NS3 protein, thus showing the functionality of this system.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extentp. 33-39pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Biotechnologypt_BR
dc.titleIntracellular Delivery of HCV NS3p gene using vectored particlespt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.jbiotec.2018.03.010pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.jbiotec.2018.03.010pt_BR
dc.contributor.externalInstituto de Biologia Experimental e Tecnológica (iBET)¦¦Portugalpt_BR
dc.identifier.citationvolume274pt_BR
dc.subject.keywordHepatitis Cpt_BR
dc.subject.keywordqPCR titrationpt_BR
dc.subject.keywordTransfectionpt_BR
dc.subject.keywordElectroporation of HEK-293T cellspt_BR
dc.subject.keywordVectored particles (VP)pt_BR
dc.relation.ispartofabbreviatedJ Biotechnolpt_BR
dc.identifier.citationabntv. 274, p. 33-39, maio 2018pt_BR
dc.identifier.citationvancouver2018 May;274:33-9pt_BR
dc.contributor.butantanSuarez-Patino, Sandra Fernanda|:Aluno|:Lab. Biotecnologia Viral|:pt_BR
dc.contributor.butantanBernardino, Thaissa Consoni|:Aluno|:Lab. Biotecnologia Viral|:pt_BR
dc.contributor.butantanAstray, Renato Mancini|:Pesquisador|:Lab. Biotecnologia Viral|:pt_BR
dc.contributor.butantanPereira, Carlos Augusto|:Pesquisador|:Lab. Biotecnologia Viral|:pt_BR
dc.contributor.butantanJorge, Soraia Attie Calil|:Pesquisador|:Lab. Biotecnologia Viral|:Autor de correspondênciapt_BR
dc.contributor.butantanLemos, Marcos Alexandre Nobre|:|:Lab. Biotecnologia Viral|:PrimeiroAutorpt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2009/08038-1pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
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