
Intracellular Delivery of HCV NS3p gene using vectored particles
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | (LABV) Laboratório de Biotecnologia Viral | pt_BR |
dc.contributor | (LMP) Laboratório Multipropósito | pt_BR |
dc.contributor.author | Lemos, Marcos Alexandre Nobre | pt_BR |
dc.contributor.author | Suarez-Patino, Sandra Fernanda | pt_BR |
dc.contributor.author | Bernardino, Thaissa Consoni | pt_BR |
dc.contributor.author | Coroadinha, Ana Sofia | pt_BR |
dc.contributor.author | Soares, Hugo | pt_BR |
dc.contributor.author | Astray, Renato Mancini | pt_BR |
dc.contributor.author | Pereira, Carlos Augusto | pt_BR |
dc.contributor.author | Jorge, Soraia Attie Calil | pt_BR |
dc.date.accessioned | 2020-07-09T21:19:52Z | - |
dc.date.available | 2020-07-09T21:19:52Z | - |
dc.date.issued | 2018 | pt_BR |
dc.identifier.citation | Lemos MAN, Suarez-Patino SF, Bernardino TC, Coroadinha AS, Soares H, Astray RM, et al. Intracellular Delivery of HCV NS3p gene using vectored particles. J. Biotechnol.. 2018 May;274:33-9. doi:10.1016/j.jbiotec.2018.03.010. | pt_BR |
dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/2452 | - |
dc.description.abstract | Viral hepatitis caused by the hepatitis C virus (HCV) affects millions of people worldwide. The non-structural protein 3 (NS3), one of the most conserved proteins in HCV, is the target of many therapeutic studies. The NS3 protease domain (NS3p) has a range of cytotoxic T lymphocyte (CTL) epitopes, and synthesizing the protein inside the cells is the most appropriate way to present it to the immune system. We developed a tool to study this kind of presentation, using two vectored particle (VP) systems, one based on the Semliki Forest virus (SFV) and the other on HCV pseudoparticles (HCVpp), both carrying the protease domain of the NS3 gene. In addition to producing the particles, we developed a method to quantify these VPs using qRT-PCR. We produced batches of approximately 2.4x10(4) SFV-NS3p/mu L and 4.0x10(2) HCVpp-NS3p/mu L. BHK-21 and HuH-7 cells treated with the VPs expressed the NS3 protein, thus showing the functionality of this system. | pt_BR |
dc.description.sponsorship | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo | pt_BR |
dc.description.sponsorship | (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico | pt_BR |
dc.format.extent | p. 33-39 | pt_BR |
dc.language.iso | English | pt_BR |
dc.relation.ispartof | Journal of Biotechnology | pt_BR |
dc.rights | Restricted access | pt_BR |
dc.title | Intracellular Delivery of HCV NS3p gene using vectored particles | pt_BR |
dc.type | Article | pt_BR |
dc.identifier.doi | 10.1016/j.jbiotec.2018.03.010 | pt_BR |
dc.identifier.url | http://dx.doi.org/10.1016/j.jbiotec.2018.03.010 | pt_BR |
dc.contributor.external | (iBET) Instituto de Biologia Experimental e Tecnológica | pt_BR |
dc.identifier.citationvolume | 274 | pt_BR |
dc.subject.keyword | Hepatitis C | pt_BR |
dc.subject.keyword | qPCR titration | pt_BR |
dc.subject.keyword | Transfection | pt_BR |
dc.subject.keyword | Electroporation of HEK-293T cells | pt_BR |
dc.subject.keyword | Vectored particles (VP) | pt_BR |
dc.relation.ispartofabbreviated | J Biotechnol | pt_BR |
dc.identifier.citationabnt | v. 274, p. 33-39, maio 2018 | pt_BR |
dc.identifier.citationvancouver | 2018 May;274:33-9 | pt_BR |
dc.contributor.butantan | Suarez-Patino, Sandra Fernanda|:Aluno|:Lab. Biotecnologia Viral|: | pt_BR |
dc.contributor.butantan | Bernardino, Thaissa Consoni|:Aluno|:Lab. Biotecnologia Viral|: | pt_BR |
dc.contributor.butantan | Astray, Renato Mancini|:Pesquisador|:Lab. Biotecnologia Viral|: | pt_BR |
dc.contributor.butantan | Pereira, Carlos Augusto|:Pesquisador|:Lab. Biotecnologia Viral|: | pt_BR |
dc.contributor.butantan | Jorge, Soraia Attie Calil|:Pesquisador|:Lab. Biotecnologia Viral|:Autor de correspondência | pt_BR |
dc.contributor.butantan | Lemos, Marcos Alexandre Nobre|:|:Lab. Biotecnologia Viral|:PrimeiroAutor | pt_BR |
dc.sponsorship.butantan | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦ | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2009/08038-1 | pt_BR |
dc.identifier.bvscc | BR78.1 | pt_BR |
dc.identifier.bvsdb | IBProd | pt_BR |
dc.description.dbindexed | Yes | pt_BR |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | English | - |
item.grantfulltext | embargo_29990101 | - |
item.openairetype | Article | - |
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crisitem.author.orcid | 0000-0002-6983-5298 | - |
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crisitem.author.orcid | 0000-0001-9380-1023 | - |
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