Signaling pathways involved in zymosan phagocytosis induced by two secreted phospholipases A(2) isolated from Bothrops asper snake venom in macrophages

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dc.contributorLaboratório de Farmacologiapt_BR
dc.contributor.authorZuliani, Juliana Pavanpt_BR
dc.contributor.authorMaria Gutierrez, Josept_BR
dc.contributor.authorTeixeira, Catarina de Fátima Pereirapt_BR
dc.date.accessioned2020-07-09T21:19:59Z-
dc.date.available2020-07-09T21:19:59Z-
dc.date.issued2018-
dc.identifier.citationZuliani JP, Maria Gutierrez J, Teixeira CFP. Signaling pathways involved in zymosan phagocytosis induced by two secreted phospholipases A(2) isolated from Bothrops asper snake venom in macrophages. Int. J. Biol. Macromol.. 2018 Jul;113:575-82. doi:10.1016/j.ijbiomac.2018.02.158.pt_BR
dc.identifier.issn0141-8130-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2461-
dc.description.abstractPhagocytosis, a process involved in host defense, requires coordination of a variety of signaling reactions. MT-II, a catalytically-inactive Lys49-PLA(2), and MT-III, an active Asp49-PLA(2) isolated from Bothrops asper snake venom, activate phagocytosis in macrophages. In this study the signal pathways mediating zymosan phagocytosis, focusing in lipidic second messengers, were investigated. Macrophages collected from male Swiss mouse peritoneum were obtained 96 h after i.p. injection of thioglycollate. Phagocytosis was evaluated with non-opsonized zymosan in the presence or absence of specific inhibitors. Data showed that both venom PLA(2)s increased phagocytosis. Zileuton, Etoricoxib, PACOCF3 (5-LO, COX-2 and iPLA(2) inhibitors, respectively), as well as WEB2170 (PAF receptor antagonist) significantly reduced phagocytosis induced by both venom PLA(2)s. However, Indomethacin (COX-1/COX-2 inhibitor) and Montelukast (CysL receptor antagonist) did not affect the toxins-induced phagocytosis. Moreover, while PACOCF3 (iPLA(2) inhibitor), reduced the phagocytosis induced by MT-II and MT-III, AACOCF3 (cPLA(2) inhibitor) significantly reduced the MT-II, but not MT-Ill-induced phagocytosis. These data suggest the effect of both sPLA(2)s depends on IPLA(2) and that the effect of MT-II depends on activation of cPLA(2). COX-2 and 5-W-derived metabolites as well as PAF are involved in the signaling events required for phagocytosis induced by both venom sPLA(2)s.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extentp. 575-582pt_BR
dc.languageengpt_BR
dc.publisherElsevier Science BVpt_BR
dc.relation.ispartofInternational Journal of Biological Macromoleculespt_BR
dc.titleSignaling pathways involved in zymosan phagocytosis induced by two secreted phospholipases A(2) isolated from Bothrops asper snake venom in macrophagespt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.ijbiomac.2018.02.158pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.ijbiomac.2018.02.158pt_BR
dc.contributor.externalFundação Oswaldo Cruz (Fiocruz)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de Rondônia (UNIR)¦¦Brasilpt_BR
dc.contributor.externalUniversidad de Costa Rica (UCR)¦¦Costa Ricapt_BR
dc.publisher.cityAmsterdampt_BR
dc.identifier.citationvolume113pt_BR
dc.subject.keywordVenom PLA2pt_BR
dc.subject.keywordMacrophagespt_BR
dc.subject.keywordPhagocytosispt_BR
dc.subject.keywordSignal transductionpt_BR
dc.relation.ispartofabbreviatedInt. J. Biol. Macromol.pt_BR
dc.identifier.citationabntv. 113, p. 575-582, jul. 2018pt_BR
dc.identifier.citationvancouver2018 Jul;113:575-82pt_BR
dc.publisher.countryNetherlandspt_BR
dc.contributor.butantanZuliani, Juliana Pavan|:Pesquisador|:Laboratório de Farmacologia|:PrimeiroAutorpt_BR
dc.contributor.butantanTeixeira, Catarina de Fátima Pereira|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Farmacologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦301199/91-4pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦307379/2016-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦02/13863-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦02/01009-7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
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