Knockdown of NF-kB1 by shRNA Inhibits the Growth of Renal Cell Carcinoma In Vitro and In Vivo

Full metadata record
DC FieldValueLanguage
dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributorCeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.authorIkegami, Amandapt_BR
dc.contributor.authorTeixeira, Luiz Felipe S.pt_BR
dc.contributor.authorBraga, Marina S.pt_BR
dc.contributor.authorDias, Matheus Henriquept_BR
dc.contributor.authorLopes, Eduardo Cararopt_BR
dc.contributor.authorBellini, Maria Helenapt_BR
dc.date.accessioned2020-07-09T21:20:23Z-
dc.date.available2020-07-09T21:20:23Z-
dc.date.issued2018pt_BR
dc.identifier.citationIkegami A, Teixeira LFS., Braga MS., Dias MH, Lopes EC, Bellini MH. Knockdown of NF-kB1 by shRNA Inhibits the Growth of Renal Cell Carcinoma In Vitro and In Vivo. Oncol. Res.. 2018;26(5): 743-51. doi:10.3727/096504017X15120379906339.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2490-
dc.description.abstractRenal cell carcinoma (RCC) accounts for approximately 2%-3% of human malignancies and is the most aggressive among urologic tumors. Biological heterogeneity, drug resistance, and chemotherapy side effects are the biggest obstacles to the effective treatment of RCC. The NF-kappa B transcription factor is one of several molecules identified to be responsible for the aggressive phenotype of this tumor. In the past decade, several studies have demonstrated the activation of NF-kappa B in RCC, and many have implicated NF-kappa B1 (p50) as an important molecule in tumor progression and metastasis. In the present study, a lentivirus was used to deliver shRNA targeting NF-kappa B1 into mouse RCC (Renca) cells. It was determined that the knockdown of the NF-kappa B1 gene led to a reduction in cell proliferation and late apoptosis/necrosis in vitro. Flow cytometry analysis demonstrated G(2)/M arrest in the cells. In addition, immunoblotting analysis revealed a significant increase in cyclin B1 and Bax. In vivo experiments showed that Renca-shRNA-NF-kappa B1 cells have significantly diminished tumori genicity. Moreover, immunohistochemical analysis revealed an increase in necrotic areas of Renca-shRNA-NF-kappa B1 tumors. Thus, this study indicates that downregulation of NF-kappa B1 can suppress RCC tumorigenesis by inducing late apoptosis/necrosis. Therefore, NF-kappa B1 may be a potential therapeutic target for RCC.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extentp. 743-751pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofOncology Researchpt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/pt_BR
dc.titleKnockdown of NF-kB1 by shRNA Inhibits the Growth of Renal Cell Carcinoma In Vitro and In Vivopt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BY-NC-NDpt_BR
dc.identifier.doi10.3727/096504017X15120379906339pt_BR
dc.identifier.urlhttp://dx.doi.org/10.3727/096504017X15120379906339pt_BR
dc.contributor.externalInstituto de Pesquisas Energéticas e Nucleares (IPEN)¦¦Brasilpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.identifier.citationvolume26pt_BR
dc.identifier.citationissue5pt_BR
dc.subject.keywordRenal cell carcinoma (RCC)pt_BR
dc.subject.keywordProliferationpt_BR
dc.subject.keywordShort hairpin RNA (shRNA)pt_BR
dc.subject.keywordNuclear factor kappa-light-chain-enhancer of activated B cells 1 (NF-kappa B1)pt_BR
dc.relation.ispartofabbreviatedOncol Respt_BR
dc.identifier.citationabntv. 26. n. 5, p. 743-751, 2018pt_BR
dc.identifier.citationvancouver2018;26(5): 743-51pt_BR
dc.contributor.butantanDias, Matheus Henrique|:Aluno|:Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celular|:pt_BR
dc.contributor.butantanLopes, Eduardo Cararo|:Aluno|:Laboratório de Toxinologia Aplicada:CeTICS - Centro de Toxinas, Resposta-imune e Sinalização Celular|:pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/19265-7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
item.grantfulltextopen-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

OR-26-743.pdf
Size: 9.11 MB
Format: Adobe PDF
View/Open
Show simple item record

This item is licensed under a Creative Commons License Creative Commons