A carbohydrate moiety of secreted stage-specific glycoprotein 4 participates in host cell invasion by Trypanosoma cruzi extracellular amastigotes

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dc.contributorLCC - Laboratório de Ciclo Celularpt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorFlorentino, Pilar T. V.pt_BR
dc.contributor.authorReal, Fernandopt_BR
dc.contributor.authorOrikaza, Cristina M.pt_BR
dc.contributor.authorda Cunha, Julia Pinheiro Chagaspt_BR
dc.contributor.authorVitorino, Francisca Nathália de Lunapt_BR
dc.contributor.authorCordero, Esteban M.pt_BR
dc.contributor.authorSobreira, Tiago J. P.pt_BR
dc.contributor.authorMortara, Renato A.pt_BR
dc.date.accessioned2020-07-09T21:20:36Z-
dc.date.available2020-07-09T21:20:36Z-
dc.date.issued2018pt_BR
dc.identifier.citationFlorentino PT.V., Real F, Orikaza CM., da Cunha JPC, Vitorino FNL, Cordero EM., et al. A carbohydrate moiety of secreted stage-specific glycoprotein 4 participates in host cell invasion by Trypanosoma cruzi extracellular amastigotes. Front. Microbiol.. 2018;9:693. doi:10.3389/fmicb.2018.00693.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2505-
dc.description.abstractTrypanosoma cruzi is the etiologic agent of Chagas' disease. It is known that amastigotes derived from trypomastigotes in the extracellular milieu are infective in vitro and in vivo. Extracellular amastigotes (EAs) have a stage-specific surface antigen called Ssp-4, a GPI-anchored glycoprotein that is secreted by the parasites. By immunoprecipitation with the Ssp-4-specific monoclonal antibodies (mAb) 2C2 and 1D9, we isolated the glycoprotein from EAs. By mass spectrometry, we identified the core protein of Ssp-4 and evaluated mRNA expression and the presence of Ssp-4 carbohydrate epitopes recognized by mAb1D9. We demonstrated that the carbohydrate epitope recognized by mAb1D9 could promote host cell invasion by EAs. Although infectious EAs express lower amounts of Ssp-4 compared with less-infectious EAs (at the mRNA and protein levels), it is the glycosylation of Ssp-4 (identified by mAb1D9 staining only in infectious strains and recognized by galectin-3 on host cells) that is the determinant of EA invasion of host cells. Furthermore, Ssp-4 is secreted by EAs, either free or associated with parasite vesicles, and can participate in host-cell interactions. The results presented here describe the possible role of a carbohydrate moiety of T. cruzi surface glycoproteins in host cell invasion by EA forms, highlighting the potential of these moieties as therapeutic and vaccine targets for the treatment of Chagas' disease.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent693pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Microbiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleA carbohydrate moiety of secreted stage-specific glycoprotein 4 participates in host cell invasion by Trypanosoma cruzi extracellular amastigotespt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3389/fmicb.2018.00693pt_BR
dc.identifier.urlhttp://dx.doi.org/10.3389/fmicb.2018.00693pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalInstitut National de la Santé et de la Recherche Médicale (INSERM)¦¦Françapt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.contributor.externalUniversidad Mayor¦¦Chilept_BR
dc.contributor.externalPurdue University¦¦Estados Unidospt_BR
dc.identifier.citationvolume9pt_BR
dc.subject.keywordTrypanosoma cruzipt_BR
dc.subject.keywordextracellular amastigotespt_BR
dc.subject.keywordSsp-4 glycoproteinpt_BR
dc.subject.keywordgalectin-3pt_BR
dc.relation.ispartofabbreviatedFront Microbiolpt_BR
dc.identifier.citationabntv. 9, 693, 2018pt_BR
dc.identifier.citationvancouver2018;9:693pt_BR
dc.contributor.butantanda Cunha, Julia Pinheiro Chagas|:Pesquisador|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanVitorino, Francisca Nathália de Luna|:Aluno|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦302068/2016-3pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2011/51475-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2012/23150-5pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
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item.languageiso639-1English-
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