Genotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, on MDA-MB-231 breast cancer cells

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dc.contributorLCC - Laboratório de Ciclo Celularpt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorSilva, Makswell Ferreira S.pt_BR
dc.contributor.authorLopes, Daiana Silvapt_BR
dc.contributor.authorTeixeira, Samuel Cotapt_BR
dc.contributor.authorGimenes, Sarah Natalie Cirilopt_BR
dc.contributor.authorAzevedo, Fernanda Van Petten Vasconcelospt_BR
dc.contributor.authorPolloni, Lorenapt_BR
dc.contributor.authorBorges, Bruna Cristinapt_BR
dc.contributor.authorSilva, Marcelo Santos dapt_BR
dc.contributor.authorBarbosa, Marcelo Josépt_BR
dc.contributor.authorOliveira Júnior, Robson José dept_BR
dc.contributor.authorElias, Maria Carolinapt_BR
dc.contributor.authorDa Silva, Cláudio Vieirapt_BR
dc.contributor.authorYoneyama, Kelly Aparecida Geraldopt_BR
dc.contributor.authorRodrigues, Veridiana De Melopt_BR
dc.contributor.authorRodrigues, Renata Santospt_BR
dc.date.accessioned2020-07-09T21:20:54Z-
dc.date.available2020-07-09T21:20:54Z-
dc.date.issued2018pt_BR
dc.identifier.citationSilva MFS., Lopes DS, Teixeira SC, Gimenes SNC, Azevedo FVPV, Polloni L, et al. Genotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, on MDA-MB-231 breast cancer cells. Int. J. Biol. Macromol.. 2018 Oct;118(part A):311-9. doi:10.1016/j.ijbiomac.2018.06.082.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2529-
dc.description.abstractHerein we evaluated the genotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2) from Bothrops pauloensis, on breast cancer cells. BnSP-6 was able to induce a higher cytotoxic and genotoxic activity in MDA-MB-231 cells, when compared to MCF10A (a non-tumorigenic breast cell line), suggesting that this protein presented a possible preference for cancer cells. BnSP-6 inhibited MDA-MB-231 proliferation at 24, 48 and 72?h. In addition, BnSP-6 induced significant increase in the percentage of TUNEL-positive cells, a marker of DNA damage. To obtain novel insight into the direct DNA damage interference in MDA-MB-231 survival and proliferation, we evaluated cell cycle progression. BnSP-6 produced a significant decrease in 2N (G1) and an increase in the G2/M phase and this capacity is likely related to the modulation of expression of progression cell cycle-associated genes (CCND1, CCNE1, CDC25A, CHEK2, E2F1, CDH-1 and NF-kB). Taken together, these results indicate that BnSP-6 induces DNA damage in breast cancer cells and is an attractive model for developing innovative therapeutic agents against breast cancer.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extentp. 311-319pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofInternational Journal of Biological Macromoleculespt_BR
dc.titleGenotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, on MDA-MB-231 breast cancer cellspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.ijbiomac.2018.06.082pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.ijbiomac.2018.06.082pt_BR
dc.contributor.externalUniversidade Federal de Uberlândia (UFU)¦¦Brasilpt_BR
dc.identifier.citationvolume118pt_BR
dc.subject.keywordPLA2pt_BR
dc.subject.keywordBnSP-6pt_BR
dc.subject.keywordBreast cancerpt_BR
dc.subject.keywordGenotoxicitypt_BR
dc.subject.keywordCell cyclept_BR
dc.subject.keywordBothrops pauloensispt_BR
dc.relation.ispartofabbreviatedInt J Biol Macromolpt_BR
dc.identifier.citationabntv. 118, part A, p. 311-319, out. 2018pt_BR
dc.identifier.citationvancouver2018 Oct;118(part A):311-9pt_BR
dc.contributor.butantanSilva, Marcelo Santos da|:Aluno|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanElias, Maria Carolina|:Pesquisador|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦CNPq-465669/2014-0pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦CBB-APQ-01637-15pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦CBB - APQ-01401-17pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/24170-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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