Genotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, on MDA-MB-231 breast cancer cells
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | Laboratório Especial de Ciclo Celular | pt_BR |
dc.contributor | Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS) | pt_BR |
dc.contributor.author | Silva, Makswell Ferreira S. | pt_BR |
dc.contributor.author | Lopes, Daiana Silva | pt_BR |
dc.contributor.author | Teixeira, Samuel Cota | pt_BR |
dc.contributor.author | Gimenes, Sarah Natalie Cirilo | pt_BR |
dc.contributor.author | Azevedo, Fernanda Van Petten Vasconcelos | pt_BR |
dc.contributor.author | Polloni, Lorena | pt_BR |
dc.contributor.author | Borges, Bruna Cristina | pt_BR |
dc.contributor.author | Silva, Marcelo Santos da | pt_BR |
dc.contributor.author | Barbosa, Marcelo José | pt_BR |
dc.contributor.author | Oliveira Júnior, Robson José de | pt_BR |
dc.contributor.author | Elias, Maria Carolina | pt_BR |
dc.contributor.author | Da Silva, Cláudio Vieira | pt_BR |
dc.contributor.author | Yoneyama, Kelly Aparecida Geraldo | pt_BR |
dc.contributor.author | Rodrigues, Veridiana De Melo | pt_BR |
dc.contributor.author | Rodrigues, Renata Santos | pt_BR |
dc.date.accessioned | 2020-07-09T21:20:54Z | - |
dc.date.available | 2020-07-09T21:20:54Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Silva MFS., Lopes DS, Teixeira SC, Gimenes SNC, Azevedo FVPV, Polloni L, et al. Genotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, on MDA-MB-231 breast cancer cells. Int. J. Biol. Macromol.. 2018 Oct;118(part A):311-9. doi:10.1016/j.ijbiomac.2018.06.082. | pt_BR |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/2529 | - |
dc.description.abstract | Herein we evaluated the genotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2) from Bothrops pauloensis, on breast cancer cells. BnSP-6 was able to induce a higher cytotoxic and genotoxic activity in MDA-MB-231 cells, when compared to MCF10A (a non-tumorigenic breast cell line), suggesting that this protein presented a possible preference for cancer cells. BnSP-6 inhibited MDA-MB-231 proliferation at 24, 48 and 72?h. In addition, BnSP-6 induced significant increase in the percentage of TUNEL-positive cells, a marker of DNA damage. To obtain novel insight into the direct DNA damage interference in MDA-MB-231 survival and proliferation, we evaluated cell cycle progression. BnSP-6 produced a significant decrease in 2N (G1) and an increase in the G2/M phase and this capacity is likely related to the modulation of expression of progression cell cycle-associated genes (CCND1, CCNE1, CDC25A, CHEK2, E2F1, CDH-1 and NF-kB). Taken together, these results indicate that BnSP-6 induces DNA damage in breast cancer cells and is an attractive model for developing innovative therapeutic agents against breast cancer. | pt_BR |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | pt_BR |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | pt_BR |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) | pt_BR |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | pt_BR |
dc.format.extent | p. 311-319 | pt_BR |
dc.language | eng | pt_BR |
dc.publisher | Elsevier Science BV | pt_BR |
dc.relation.ispartof | International Journal of Biological Macromolecules | pt_BR |
dc.title | Genotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, on MDA-MB-231 breast cancer cells | pt_BR |
dc.type | Article | pt_BR |
dc.identifier.doi | 10.1016/j.ijbiomac.2018.06.082 | pt_BR |
dc.identifier.url | http://dx.doi.org/10.1016/j.ijbiomac.2018.06.082 | pt_BR |
dc.contributor.external | Universidade Federal de Uberlândia (UFU)¦¦Brasil | pt_BR |
dc.publisher.city | Amsterdam | pt_BR |
dc.identifier.citationvolume | 118 | pt_BR |
dc.subject.keyword | PLA2 | pt_BR |
dc.subject.keyword | BnSP-6 | pt_BR |
dc.subject.keyword | Breast cancer | pt_BR |
dc.subject.keyword | Genotoxicity | pt_BR |
dc.subject.keyword | Cell cycle | pt_BR |
dc.subject.keyword | Bothrops pauloensis | pt_BR |
dc.relation.ispartofabbreviated | Int. J. Biol. Macromol. | pt_BR |
dc.identifier.citationabnt | v. 118, part A, p. 311-319, out. 2018 | pt_BR |
dc.identifier.citationvancouver | 2018 Oct;118(part A):311-9 | pt_BR |
dc.publisher.country | Netherlands | pt_BR |
dc.contributor.butantan | Silva, Marcelo Santos da|:Aluno|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|: | pt_BR |
dc.contributor.butantan | Elias, Maria Carolina|:Pesquisador|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|: | pt_BR |
dc.sponsorship.butantan | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦CNPq-465669/2014-0 | pt_BR |
dc.sponsorship.butantan | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦ | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦CBB-APQ-01637-15 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦CBB - APQ-01401-17 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/24170-5 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1 | pt_BR |
dc.identifier.bvscc | BR78.1 | pt_BR |
dc.identifier.bvsdb | IBProd | pt_BR |
item.openairetype | Article | - |
item.fulltext | Sem Texto completo | - |
item.grantfulltext | none | - |
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