Presynaptic activity of an isolated fraction from Rhinella schneideri poison

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dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorRostelato-Ferreira, Sandropt_BR
dc.contributor.authorDal Belo, Cháriston Andrépt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.contributor.authorHyslop, Stephenpt_BR
dc.contributor.authorRodrigues-Simioni, Léapt_BR
dc.contributor.authorRocha-e-Silva, Thomaz Augusto Alvespt_BR
dc.identifier.citationRostelato-Ferreira S, Dal Belo CA, Silva Junior PI, Hyslop S, Rodrigues-Simioni L, Rocha-e-Silva TAA. Presynaptic activity of an isolated fraction from Rhinella schneideri poison. Adv Pharm Bull. 2018;8(3):517-22. doi:10.15171/apb.2018.060.pt_BR
dc.description.abstractPurpose: Rhinella schneideri is a toad found in many regions of the South America. The poison of the glands has cardiotoxic effect in animals and neuromuscular effects in mice and avian preparation. The purpose of this work was to identify the toxin responsible for the neuromuscular effect in avian and mice neuromuscular preparation. Methods: The methanolic extract from R. schneideri poison was fractioned by reversed phase HPLC. The purity and molecular mass were determined by LC/MS mass spectrometry. Chick biventer cervicis and mouse phrenic-nerve diaphragm were used as neuromuscular preparations to identify the toxin. Results: The purification resulted in 32 fractions, which 4 of them were active in neuromuscular preparation. The toxin of fraction 20 were chosen for better reproducibility of the whole extract activity and its molecular mass was 730.6 Da. The toxin produced facilitation of the muscle contraction followed by a complete neuromuscular blockade in chick biventer cervicis preparation in 90 min without interfering with the exogenous response to ACh and KCl. The quantal content was increased from 128 ± 13 (control) to 216 ± 44 (after 5 min and sustained until 60 min) in the presence of the toxin. Conclusion: In conclusion, our results demonstrated that the neuromuscular action of the poison of Rhinella schneideri is a multitoxin effect. More, the present work first isolated a 730.6 Da toxin that better represent the whole poison neuromuscular effect, to which is attributed a presynaptic action in avian and mouse neuromuscular preparation.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extentp. 517-522pt_BR
dc.relation.ispartofAdvanced Pharmaceutical Bulletinpt_BR
dc.rightsOpen Accesspt_BR
dc.titlePresynaptic activity of an isolated fraction from Rhinella schneideri poisonpt_BR
dc.contributor.externalUniversidade Estadual de Campinas (UNICAMP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Paulista (UNIP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal do Pampa (UNIPAMPA)¦¦Brasilpt_BR
dc.contributor.externalHospital Israelita Albert Einstein¦¦Brasilpt_BR
dc.subject.keywordNeurotransmitter releasept_BR
dc.subject.keywordPresynaptic toxinpt_BR
dc.subject.keywordNeuromuscular junctionpt_BR
dc.subject.keywordIsolated fractionpt_BR
dc.relation.ispartofabbreviatedAdv Pharm Bullpt_BR
dc.identifier.citationabntv. 8, n. 3, p. 517-522, 2018pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦201342/2010-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦08/54050-0pt_BR
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