Human antimicrobial peptide isolated from Triatoma infestans haemolymph, Trypanosoma cruzi-transmitting vector

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dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorDiniz, Laura Cristina Limapt_BR
dc.contributor.authorMiranda, Antoniopt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.date.accessioned2020-07-09T21:22:01Z-
dc.date.available2020-07-09T21:22:01Z-
dc.date.issued2018pt_BR
dc.identifier.citationDiniz LCL, Miranda A, Silva Junior PI. Human antimicrobial peptide isolated from Triatoma infestans haemolymph, Trypanosoma cruzi-transmitting vector. Front. Cell. Infect. Microbiol.. 2018;8:354. doi:10.3389/fcimb.2018.00354.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2611-
dc.description.abstractThe importance of antimicrobial peptides (AMPs) in relation to the survival of invertebrates is well known. The source and the mode of action on the insects' immune system of these molecules have been described from different perspectives. Insects produce their own AMPs as well as obtain these molecules from various sources, for example by absorption through the intestinal tract, as previously described for Boophilus microplus. Blood-sucking barber bug Triatoma infestans attracts social, economic and medical interest owing to its role in the transmission of Chagas disease. Despite new studies, descriptions of AMPs from this insect have remained elusive. Thus, the aims of this work were to characterize the antimicrobial potential of human fibrinopeptide A (FbPA) obtained from the T. infestans haemolymph and identify its natural source. Therefore, FbPA was isolated from the T. infestans haemolymph through liquid chromatography and identified by mass spectrometry. This peptide exhibited antimicrobial activity against Micrococcus luteus. Native FbPA from human blood and the synthetic FbPA also exhibited antimicrobial activity. The synthetic FbPA was conjugated with fluorescein isothiocyanate and offered to the insects. The haemolymph collected after 72 h exhibited fluorescence at the same wavelength as fluorescein isothiocyanate. Our experiments show that beyond intrinsic AMP production, T. infestans is able to co-opt molecules via internalization and may use them as AMPs for protection.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent354pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Cellular and Infection Microbiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleHuman antimicrobial peptide isolated from Triatoma infestans haemolymph, Trypanosoma cruzi-transmitting vectorpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3389/fcimb.2018.00354pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fcimb.2018.00354pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.identifier.citationvolume8pt_BR
dc.subject.keywordantimicrobial peptidespt_BR
dc.subject.keywordTriatoma infestanspt_BR
dc.subject.keywordfibrinopeptide Apt_BR
dc.subject.keywordinnate immune systempt_BR
dc.subject.keywordinternalizationpt_BR
dc.relation.ispartofabbreviatedFront Cell Infect Microbiolpt_BR
dc.identifier.citationabntv. 8, 354, 2018pt_BR
dc.identifier.citationvancouver2018;8:354pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA)|:Autor de correspondênciapt_BR
dc.contributor.butantanDiniz, Laura Cristina Lima|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:PrimeiroAutorpt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦130409/2014-6pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦472744/2012-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1English-
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