Venom of the Phoneutria nigriventer spider alters the cell cycle, viability, and migration of cancer cells

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dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorSantos, Natália Barreto dospt_BR
dc.contributor.authorBonfanti, Amanda Pirespt_BR
dc.contributor.authorRocha-e-Silva, Thomaz Augusto Alves dapt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.contributor.authorCruz-Hofling, Maria Alice dapt_BR
dc.contributor.authorVerinaud, Lianapt_BR
dc.contributor.authorRapôso, Catarinapt_BR
dc.date.accessioned2020-07-09T21:22:09Z-
dc.date.available2020-07-09T21:22:09Z-
dc.date.issued2019pt_BR
dc.identifier.citationSantos NB, Bonfanti AP, Rocha-e-Silva TAA, Silva Junior PI, Cruz-Hofling MA, Verinaud L, et al. Venom of the Phoneutria nigriventer spider alters the cell cycle, viability, and migration of cancer cells. J. Cell. Physiol.. 2019 Feb;234(2):1398-1415. doi:10.1002/jcp.26935.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2616-
dc.description.abstractThe mechanisms of cancer involve changes in multiple biological pathways. Multitarget molecules, which are components of animal venoms, are therefore a potential strategy for treating tumors. The objective of this study was to screen the effects of Phoneutria nigriventer spider venom (PnV) on tumor cell lines. Cultured human glioma (NG97), glioblastoma (U-251) and cervix adenocarcinoma (HeLa) cells, and nontumor mouse fibroblasts (L929) were treated with low (14?µg/ml) and high (280?µg/ml) concentrations of PnV, and analyzed through assays for cell viability (thiazolyl blue tetrazolium blue), proliferation (carboxyfluorescein succinimidyl ester), death (annexin V/propidium iodide [Pi]), the cell cycle (Pi), and migration (wound healing and transwell assay). The venom decreased the viability of U-251 cells, primarily by inducing cell death, and reduced the viability of NG97 cells, primarily by inhibiting the cell cycle. The migration of all the tumor cell lines was delayed when treated with venom. The venom significantly affected all the tumor cell lines studied, with no cytotoxic effect on normal cells (L929), although the nonglial tumor cell (HeLa) was less sensitive to PnV. The results of the current study suggest that PnV may be composed of peptides that are highly specific for the multiple targets involved in the hallmarks of cancer. Experiments are underway to identify these molecules.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extentp. 1398-1415pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Cellular Physiologypt_BR
dc.titleVenom of the Phoneutria nigriventer spider alters the cell cycle, viability, and migration of cancer cellspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1002/jcp.26935pt_BR
dc.identifier.urlhttps://doi.org/10.1002/jcp.26935pt_BR
dc.contributor.external(UNICAMP) Universidade Estadual de Campinaspt_BR
dc.contributor.externalFaculdade Israelita de Ciências da Saúde Albert Einstein¦¦Brasilpt_BR
dc.identifier.citationvolume234pt_BR
dc.identifier.citationissue2pt_BR
dc.subject.keywordarthropod venompt_BR
dc.subject.keywordcancer therapypt_BR
dc.subject.keywordin vitropt_BR
dc.subject.keywordmigrationpt_BR
dc.subject.keywordproliferationpt_BR
dc.relation.ispartofabbreviatedJ Cell Physiolpt_BR
dc.identifier.citationabntv. 234, n. 2, p. 1398-1415, fev. 2019pt_BR
dc.identifier.citationvancouver2019 Feb;234(2):1398-1415pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório de Toxinologia Aplicada|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦431465/2016-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/04194-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/15827-6pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16196-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦017/05402-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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