Preclinical evaluation of Amblyomin-X, a Kunitz-type protease inhibitor with antitumor activity

Full metadata record
DC FieldValueLanguage
dc.contributor(LDI) Laboratório de Desenvolvimento e Inovação Industrialpt_BR
dc.contributorLab. Imunogenéticapt_BR
dc.contributor.authorMaria, Durvanei Augustopt_BR
dc.contributor.authorWill, Sonia Elisabete Alves de Limapt_BR
dc.contributor.authorBosch, Rosemary Violapt_BR
dc.contributor.authorSouza, Jean Gabriel dept_BR
dc.contributor.authorSciani, Juliana Mozerpt_BR
dc.contributor.authorGoldfeder, Mauricio Barbugianipt_BR
dc.contributor.authorRondon, Giuliana Gagginipt_BR
dc.contributor.authorChudzinski-Tavassi, Ana Marisapt_BR
dc.date.accessioned2020-07-09T21:22:19Z-
dc.date.available2020-07-09T21:22:19Z-
dc.date.issued2019pt_BR
dc.identifier.citationMaria DA, Will SEAL, Bosch RV, Souza JG, Sciani JM, Goldfeder MB, et al. Preclinical evaluation of Amblyomin-X, a Kunitz-type protease inhibitor with antitumor activity. Toxicol. Rep.. 2019;6:51-63. doi:10.1016/j.toxrep.2018.11.014.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2630-
dc.description.abstractAmblyomin-X, a Kunitz-type protease inhibitor, is a recombinant protein that selectively induces apoptosis in tumor cells and promotes tumor reduction in vivo in melanoma animal models. Furthermore, Amblyomin-X was able to drastically reduce lung metastasis in a mice orthotopic kidney tumor model. Due to its antitumor activity, Amblyomin-X potential to become a new drug is currently under investigation, therefore the aim of the present study was to perform preclinical assays to evaluate Amblyomin-X toxicity in healthy mice. Exploratory toxicity assays have shown that treatment with 512?mg/kg of Amblyomin-X lead to animal mortality, therefore two groups of treatment were evaluated in the present work: in the acute toxicity assay, animals were injected once with doses ranging from 4 to 256?mg/kg of Amblyomin-X, while in the subacute toxicity assay, animals were injected with 0.25, 0.57 and 1?mg/kg of Amblyomin-X daily, during 28 days. Following this treatment regimens, Amblyomin-X did not cause any mortality; moreover, toxicity signs were discrete, reversible and observed only at the higher doses, thus establishing a safety profile for administration in mice, which can be further used to determine the dose translation of this novel drug candidate for treatment in other species.pt_BR
dc.format.extentp. 51-63pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxicology Reportspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/pt_BR
dc.titlePreclinical evaluation of Amblyomin-X, a Kunitz-type protease inhibitor with antitumor activitypt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BY-NC-NDpt_BR
dc.identifier.doi10.1016/j.toxrep.2018.11.014pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.toxrep.2018.11.014pt_BR
dc.identifier.citationvolume6pt_BR
dc.subject.keywordamblyomin-Xpt_BR
dc.subject.keywordKunitz-type inhibitorpt_BR
dc.subject.keywordtoxicitypt_BR
dc.subject.keywordPreclinicalpt_BR
dc.relation.ispartofabbreviatedToxicol Reppt_BR
dc.identifier.citationabntv. 6, p. 51-63, 2019pt_BR
dc.identifier.citationvancouver2019;6:51-63pt_BR
dc.contributor.butantanMaria, Durvanei Augusto|:Pesquisador|:Lab. Biologia Molecular|:PrimeiroAutorpt_BR
dc.contributor.butantanSouza, Jean Gabriel de|:Aluno|:Lab. Imunogenética:Lab. Biologia Molecular|:pt_BR
dc.contributor.butantanSciani, Juliana Mozer|:Técnico:Docente Colaborador PPGTOX|:Lab. Biologia Molecular|:pt_BR
dc.contributor.butantanChudzinski-Tavassi, Ana Marisa|:Pesquisador:Docente Permanente PPGTOX|:Lab. Biologia Molecular|:Autor de correspondênciapt_BR
dc.contributor.butantanWill, Sonia Elisabete Alves de Lima|:Aluno|:Lab. Biologia Molecular|:pt_BR
dc.contributor.butantanBosch, Rosemary Viola|:Aluno|:Lab. Biologia Molecular|:pt_BR
dc.contributor.butantanGoldfeder, Mauricio Barbugiani|:Técnico|:Lab. Biologia Molecular|:pt_BR
dc.contributor.butantanRondon, Giuliana Gaggini|:Aluno|:Lab. Biologia Molecular|:pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.subject.researchlineBioprospecção e desenvolvimentopt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.languageiso639-1English-
item.grantfulltextopen-
item.openairetypeArticle-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-4120-8468-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0001-7213-206X-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0001-7717-7013-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

10.1016j.toxrep.2018.11.014.pdf
Size: 8.21 MB
Format: Adobe PDF
View/Open
Show simple item record

This item is licensed under a Creative Commons License Creative Commons