Loxosceles gaucho spider venom: an untapped source of antimicrobial agents

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dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorSegura Ramirez, Paula Jimenapt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.date.accessioned2020-07-09T21:22:31Z-
dc.date.available2020-07-09T21:22:31Z-
dc.date.issued2018pt_BR
dc.identifier.citationSegura-Ramirez PJ, Silva Junior PI. Loxosceles gaucho spider venom: an untapped source of antimicrobial agents. Toxins. 2018 Dec;10(12):522. doi:10.3390/toxins10120522.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2646-
dc.description.abstractThe remarkable ability of microorganisms to develop resistance to conventional antibiotics is one of the biggest challenges that the pharmaceutical industry currently faces. Recent studies suggest that antimicrobial peptides discovered in spider venoms may be useful resources for the design of structurally new anti-infective agents effective against drug-resistant microorganisms. In this work, we found an anionic antibacterial peptide named U-1-SCRTX-Lg1a in the venom of the spider Loxosceles gaucho. The peptide was purified using high-performance liquid chromatography (HPLC), its antimicrobial activity was tested through liquid growth inhibition assays, and its chemical properties were characterized using mass spectrometry. U-1-SCRTX-Lg1a was found to show a monoisotopic mass of 1695.75 Da, activity against Gram-negative bacteria, a lack of hemolytic effects against human red blood cells, and a lack of cytotoxicity against human cervical carcinoma cells (HeLa). Besides this, the sequence of the peptide exhibited great similarity to specific regions of phospholipases D from different species of Loxosceles spiders, leading to the hypothesis that U-1-SCRTX-Lg1a may have originated from a limited proteolytic cleavage. Our data suggest that U-1-SCRTX-Lg1a is a promising candidate for the development of new antibiotics that could help fight bacterial infections and represents an exciting discovery for Loxosceles spiders.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent522pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.titleLoxosceles gaucho spider venom: an untapped source of antimicrobial agentspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3390/toxins10120522pt_BR
dc.identifier.urlhttp://dx.doi.org/10.3390/toxins10120522pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.identifier.citationvolume10pt_BR
dc.identifier.citationissue12pt_BR
dc.subject.keywordLoxoscelespt_BR
dc.subject.keywordvenompt_BR
dc.subject.keywordanionic antimicrobial peptidespt_BR
dc.subject.keywordantimicrobial resistancept_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 10, n. 12, 522, dez. 2018pt_BR
dc.identifier.citationvancouver2018 Dec;10(12):522pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA)|:Autor de correspondênciapt_BR
dc.contributor.butantanSegura Ramirez, Paula Jimena|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:PrimeiroAutorpt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦472744/2012-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1English-
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crisitem.author.orcid0000-0001-6619-6489-
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