Comparative analysis of the high molecular mass subproteomes of eight Bothrops snake venoms

Full metadata record
DC FieldValueLanguage
dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributorLCC - Laboratório de Ciclo Celularpt_BR
dc.contributor.authorGren, Eric Conrad Kylept_BR
dc.contributor.authorKitano, Eduardo Shigueopt_BR
dc.contributor.authorAndrade-Silva, Déborapt_BR
dc.contributor.authorIwai, Leo Keipt_BR
dc.contributor.authorReis, Marcelo da Silvapt_BR
dc.contributor.authorMenezes, Milene Cristinapt_BR
dc.contributor.authorSerrano, Solange Maria de Toledopt_BR
dc.date.accessioned2020-07-09T21:23:00Z-
dc.date.available2020-07-09T21:23:00Z-
dc.date.issued2019pt_BR
dc.identifier.citationGren ECK, Kitano ES, Andrade-Silva D, Iwai LK, Reis MS, Menezes MC, et al. Comparative analysis of the high molecular mass subproteomes of eight Bothrops snake venoms. Comp Biochem Physiol Part D Genomics Proteomics. 2019 Jan;30:113-121. doi:10.1016/j.cbd.2019.01.012.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2678-
dc.description.abstractSnake venoms are extremely active biological secretions composed primarily of various classes of enzymes. The genusBothropscomprises various pit viper speciesthat represent the most medically significant taxa in Central and South America, accounting for more human envenomations and fatalities than any other snakes inthe region. Venom proteomes of manyBothropsspecies have been well-characterized but investigations have focused almost exclusively on proteins smaller than100 kDa despite expression of larger components being documented in severalBothropsvenoms. This study sought to achieve detailed identification of majorcomponents in the high molecular mass subproteome of venoms from eightBothropsspecies (B.brazili,B.cotiara,B.insularis,B.jararaca,B.jararacussu,B.leucurus,B.moojeniandB. neuwiedi). Enzymes such as metalloproteinases and L-amino acid oxidases were the most prominent components identified in the first size-exclusionchromatography fractions of these venoms. Minor components also identified in the first peaks included 5'-nucleotidase, aminopeptidase, phosphodiesterase, andphospholipases A2and B. Most of these components disappeared in electrophoretic profiles under reducing conditions, suggesting that they may be composed of morethan one polypeptide chain. A significant shift in the molecular masses of these protein bands was observed following enzymatic N-deglycosylation, indicating thatthey may contain N-glycans. Furthermore, none of the identified high molecular mass proteins were shared by all eight species, revealing a high level of interspecificvariability among these venom components.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extentp. 113-121pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofComparative Biochemistry and Physiology. Part D, Genomics & Proteomicspt_BR
dc.titleComparative analysis of the high molecular mass subproteomes of eight Bothrops snake venomspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.cbd.2019.01.012pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1016/j.cbd.2019.01.012pt_BR
dc.identifier.citationvolume30pt_BR
dc.relation.ispartofabbreviatedComp Biochem Physiol Part D Genomics Proteomicspt_BR
dc.identifier.citationabntv. 30, p. 113-121, jan. 2019pt_BR
dc.identifier.citationvancouver2019 Jan;30:113-121pt_BR
dc.contributor.butantanGren, Eric Conrad Kyle|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:PrimeiroAutorpt_BR
dc.contributor.butantanKitano, Eduardo Shigueo|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanAndrade-Silva, Débora|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanReis, Marcelo da Silva|:Pesquisador|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanMenezes, Milene Cristina|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanIwai, Leo Kei|:Pesquisador:Docente Colaborador PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.contributor.butantanSerrano, Solange Maria de Toledo|:Pesquisador:Docente Permanente PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦308133/2015-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/13548-4pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/01234-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-1571-7763-
crisitem.author.orcid0000-0002-3754-9115-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0001-8994-1904-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

Existing users please Login
10.1016j.cbd.2019.01.012.pdf
Size: 2.97 MB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.