Comparative analysis of the high molecular mass subproteomes of eight Bothrops snake venoms
Full metadata record
DC Field | Value | Language |
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dc.contributor | Laboratório Especial de Toxinologia Aplicada (LETA) | pt_BR |
dc.contributor | Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS) | pt_BR |
dc.contributor | Laboratório Especial de Ciclo Celular | pt_BR |
dc.contributor.author | Gren, Eric Conrad Kyle | pt_BR |
dc.contributor.author | Kitano, Eduardo Shigueo | pt_BR |
dc.contributor.author | Andrade-Silva, Débora | pt_BR |
dc.contributor.author | Iwai, Leo Kei | pt_BR |
dc.contributor.author | Reis, Marcelo da Silva | pt_BR |
dc.contributor.author | Menezes, Milene Cristina | pt_BR |
dc.contributor.author | Serrano, Solange Maria de Toledo | pt_BR |
dc.date.accessioned | 2020-07-09T21:23:00Z | - |
dc.date.available | 2020-07-09T21:23:00Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Gren ECK, Kitano ES, Andrade-Silva D, Iwai LK, Reis MS, Menezes MC, et al. Comparative analysis of the high molecular mass subproteomes of eight Bothrops snake venoms. Comp Biochem Physiol Part D Genomics Proteomics. 2019 Jan;30:113-121. doi:10.1016/j.cbd.2019.01.012. | pt_BR |
dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/2678 | - |
dc.description.abstract | Snake venoms are extremely active biological secretions composed primarily of various classes of enzymes. The genusBothropscomprises various pit viper speciesthat represent the most medically significant taxa in Central and South America, accounting for more human envenomations and fatalities than any other snakes inthe region. Venom proteomes of manyBothropsspecies have been well-characterized but investigations have focused almost exclusively on proteins smaller than100 kDa despite expression of larger components being documented in severalBothropsvenoms. This study sought to achieve detailed identification of majorcomponents in the high molecular mass subproteome of venoms from eightBothropsspecies (B.brazili,B.cotiara,B.insularis,B.jararaca,B.jararacussu,B.leucurus,B.moojeniandB. neuwiedi). Enzymes such as metalloproteinases and L-amino acid oxidases were the most prominent components identified in the first size-exclusionchromatography fractions of these venoms. Minor components also identified in the first peaks included 5'-nucleotidase, aminopeptidase, phosphodiesterase, andphospholipases A2and B. Most of these components disappeared in electrophoretic profiles under reducing conditions, suggesting that they may be composed of morethan one polypeptide chain. A significant shift in the molecular masses of these protein bands was observed following enzymatic N-deglycosylation, indicating thatthey may contain N-glycans. Furthermore, none of the identified high molecular mass proteins were shared by all eight species, revealing a high level of interspecificvariability among these venom components. | pt_BR |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | pt_BR |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | pt_BR |
dc.format.extent | p. 113-121 | pt_BR |
dc.language | eng | pt_BR |
dc.relation.ispartof | Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics | pt_BR |
dc.title | Comparative analysis of the high molecular mass subproteomes of eight Bothrops snake venoms | pt_BR |
dc.type | Article | pt_BR |
dc.identifier.doi | https://repositorio.butantan.gov.br/handle/butantan/2678 | pt_BR |
dc.identifier.url | http://dx.doi.org/10.1016/j.cbd.2019.01.012 | pt_BR |
dc.identifier.citationvolume | 30 | pt_BR |
dc.relation.ispartofabbreviated | Comp Biochem Physiol Part D Genomics Proteomics | pt_BR |
dc.identifier.citationabnt | v. 30, p. 113-121, jan. 2019 | pt_BR |
dc.identifier.citationvancouver | 2019 Jan;30:113-121 | pt_BR |
dc.contributor.butantan | Gren, Eric Conrad Kyle|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:PrimeiroAutor | pt_BR |
dc.contributor.butantan | Kitano, Eduardo Shigueo|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|: | pt_BR |
dc.contributor.butantan | Andrade-Silva, Débora|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|: | pt_BR |
dc.contributor.butantan | Reis, Marcelo da Silva|:Pesquisador|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|: | pt_BR |
dc.contributor.butantan | Menezes, Milene Cristina|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|: | pt_BR |
dc.contributor.butantan | Iwai, Leo Kei|:Pesquisador:Docente Colaborador PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|: | pt_BR |
dc.contributor.butantan | Serrano, Solange Maria de Toledo|:Pesquisador:Docente Permanente PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|:Autor de correspondência | pt_BR |
dc.sponsorship.butantan | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦308133/2015-3 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/13548-4 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/01234-0 | pt_BR |
dc.identifier.bvscc | BR78.1 | pt_BR |
dc.identifier.bvsdb | IBProd | pt_BR |
item.openairetype | Article | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | embargo_29990101 | - |
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