Human RNF113A participates of pre-mRNA splicing in vitro

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dc.contributorLaboratório Especial de Ciclo Celularpt_BR
dc.contributor.authorSilva, Guilherme H. Gatti dapt_BR
dc.contributor.authorJurica, Melissa S.pt_BR
dc.contributor.authorda Cunha, Julia Pinheiro Chagaspt_BR
dc.contributor.authorOliveira, Carla C.pt_BR
dc.contributor.authorColtri, Patricia P.pt_BR
dc.date.accessioned2020-07-09T21:23:24Z-
dc.date.available2020-07-09T21:23:24Z-
dc.date.issued2019-
dc.identifier.citationSilva GH.G, Jurica MS., da Cunha JPC, Oliveira CC., Coltri PP.. Human RNF113A participates of pre-mRNA splicing in vitro. J Cell Biochem. 2019 May;120(5):8764-8774. doi:10.1002/jcb.28163.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2705-
dc.description.abstractPre-messenger RNA (mRNA) splicing is an essential step in the control of eukaryotic gene expression. During splicing, the introns are removed from the gene transcripts as the exons are ligated to create mature mRNA sequences. Splicing is performed by the spliceosome, which is a macromolecular complex composed of five small nuclear RNAs (snRNAs) and more than 100 proteins. Except for the core snRNP proteins, most spliceosome proteins are transiently associated and presumably involved with the regulation of spliceosome activity. In this study, we explored the association and participation of the human protein RNF113A in splicing. The addition of excess recombinant RNF113A to in vitro splicing reactions results in splicing inhibition. In whole-cell lysates, RNF113A co-immunoprecipitated with U2, U4, and U6 snRNAs, which are components of the tri-snRNP, and with proteins PRP19 and BRR2. When HeLa cells were CRISPR-edited to reduce the RNF113A levels, the in vitro splicing efficiency was severely affected. Consistently, the splicing activity was partially restored after the addition of the recombinant GST-RNF113A. On the basis on these results, we propose a model in which RNF113A associates with the spliceosome by interacting with PRP19, promoting essential rearrangements that lead to splicing.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extentp. 8764-8774pt_BR
dc.languageengpt_BR
dc.relation.ispartofJournal of Cellular Biochemistrypt_BR
dc.titleHuman RNF113A participates of pre-mRNA splicing in vitropt_BR
dc.typeArticlept_BR
dc.identifier.doihttps://repositorio.butantan.gov.br/handle/butantan/2705pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1002/jcb.28163pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversity of California (UC)¦¦Estados Unidospt_BR
dc.identifier.citationvolume120pt_BR
dc.identifier.citationissuen. 5pt_BR
dc.subject.keywordpre-messenger RNA splicingpt_BR
dc.subject.keywordRNF113Apt_BR
dc.subject.keywordspliceosomept_BR
dc.subject.keywordZNF183pt_BR
dc.relation.ispartofabbreviatedJ Cell Biochempt_BR
dc.identifier.citationabntv. 120, n. 5, p. 8764-8774, maio 2019pt_BR
dc.identifier.citationvancouver2019 May;120(5):8764-8774pt_BR
dc.contributor.butantanda Cunha, Julia Pinheiro Chagas|:Pesquisador|:Laboratório Especial de Ciclo Celular|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦474672/2013-1pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2010/51842-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2011/20664-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/02738-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/06994-9pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
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