Microorganism-based larval diets affect mosquito development, size and nutritional reserves in the yellow fever mosquito aedes aegypti (Diptera: Culicidae)

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dc.contributorLECZ - Laboratório de Coleções Zoológicaspt_BR
dc.contributorLab. Parasitologiapt_BR
dc.contributor.authorSouza, Raquel Santospt_BR
dc.contributor.authorVirginio, Fláviapt_BR
dc.contributor.authorSuesdek, Lincolnpt_BR
dc.contributor.authorRiback, Thaís Irene Souzapt_BR
dc.contributor.authorBarufi, José Bonomipt_BR
dc.contributor.authorGenta, Fernando Arielpt_BR
dc.date.accessioned2020-07-09T21:23:38Z-
dc.date.available2020-07-09T21:23:38Z-
dc.date.issued2019pt_BR
dc.identifier.citationSouza RS, Virginio F, Suesdek L, Riback TIS, Barufi JB, Genta FA. Microorganism-based larval diets affect mosquito development, size and nutritional reserves in the yellow fever mosquito aedes aegypti (Diptera: Culicidae). Front Physiol. 2019 Apr;10,152. doi:10.3389/fphys.2019.00152.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2723-
dc.description.abstractBackground: Mosquito larvae feed on organic detritus from the environment, particularly microorganisms comprising bacteria, protozoa, and algae as well as crustaceans, plant debris, and insect exuviae. Little attention has been paid to nutritional studies in Aedes aegypti larvae. Objectives: We investigated the effects of yeast, bacteria and microalgae diets on larval development, pupation time, adult size, emergence, survivorship, lifespan, and wing morphology. Materials and Methods: Microorganisms (or Tetramin® as control) were offered as the only source of food to recently hatched first instar larvae and their development was followed until the adult stage. Protein, carbohydrate, glycogen, and lipid were analyzed in single larvae to correlate energetic reserve accumulation by larva with the developmental rates and nutritional content observed. FITC-labeled microorganisms were offered to fourth instar larvae, and its ingestion was recorded by fluorescence microscopy and quantitation. Results and Discussion: Immature stages developed in all diets, however, larvae fed with bacteria and microalgae showed a severe delay in development rates, pupation time, adult emergence and low survivorship. Adult males emerged earlier as expected and had longer survival than females. Diets with better nutritional quality resulted in adults with bigger wings. Asaia sp. and Escherichia coli resulted in better nutrition and developmental parameters and seemed to be the best bacterial candidates to future studies using symbiont-based control. The diet quality was measured and presented different protein and carbohydrate amounts. Bacteria had the lowest protein and carbohydrate rates, yeasts had the highest carbohydrate amount and microalgae showed the highest protein content. Larvae fed with microalgae seem not to be able to process and store these diets properly. Larvae were shown to be able to process yeast cells and store their energetic components efficiently. Conclusion: Together, our results point that Ae. aegypti larvae show high plasticity to feed, being able to develop under different microorganism-based diets. The important role of Ae. aegypti in the spread of infectious diseases requires further biological studies in order to understand the vector physiology and thus to manage the larval natural breeding sites aiming a better mosquito control.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent152pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Physiologypt_BR
dc.titleMicroorganism-based larval diets affect mosquito development, size and nutritional reserves in the yellow fever mosquito aedes aegypti (Diptera: Culicidae)pt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3389/fphys.2019.00152pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fphys.2019.00152pt_BR
dc.contributor.externalFundação Oswaldo Cruz (Fiocruz)¦¦Brasilpt_BR
dc.contributor.externalWorld Mosquito Program¦¦Brasilpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de Santa Catarina¦¦Brasilpt_BR
dc.contributor.externalInstituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT - EM)¦¦Brasilpt_BR
dc.identifier.citationvolume10pt_BR
dc.subject.keywordAedes aegyptipt_BR
dc.subject.keywordmicroorganismpt_BR
dc.subject.keyworddevelopmentpt_BR
dc.subject.keywordnutritional reservespt_BR
dc.subject.keyworddigestionpt_BR
dc.subject.keywordyeastpt_BR
dc.subject.keywordbacteriapt_BR
dc.subject.keywordalgaept_BR
dc.relation.ispartofabbreviatedFront Physiolpt_BR
dc.identifier.citationabntv. 10, p. 152, abr. 2019pt_BR
dc.identifier.citationvancouver2019 Apr;10,152pt_BR
dc.contributor.butantanVirginio, Flávia|:Pesquisador|:LECZ - Laboratório de Coleções Zoológicas|:pt_BR
dc.contributor.butantanSuesdek, Lincoln|:Pesquisador|:Lab. Parasitologia|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
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item.grantfulltextopen-
item.languageiso639-1English-
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