The Dynamics of Replication in Trypanosoma cruzi Parasites by Single-Molecule Analysis

Here, we investigated the features of replication inTrypanosoma cruziepi-mastigotes based on fork speed progression, which is influenced by distinctfeatures such as DNA polymerase rate, susceptibility to DNA damage andrepair, secondary structures, transcription and chromatin state. AlthoughT. cruziexhibits a mean fork speed (2.050.10 kb/min) very similar to othertrypanosomatids, we found that the majority of DNA molecules replicatedmore slowly, with a frequency distribution approximately 1 kb/min. This fre-quency distribution analysis provides more information about the replicationprofile of this organism.
Keywords
DNA;  DNA replication rate;  fork speed;  inter-origin distance

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de Araujo CB, Calderano SG, Elias MC. The Dynamics of Replication in Trypanosoma cruzi Parasites by Single-Molecule Analysis. J Eukaryot Microbiol. 2019 May-Jun;66(3):514-518. doi:10.1111/jeu.12676.
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