Pep5, a fragment of Cyclin D2, shows antiparasitic effects in different stages of the Trypanosoma cruzi life cycle and blocks parasite infectivity

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dc.contributorLCC - Laboratório de Ciclo Celularpt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributorLab. Parasitologiapt_BR
dc.contributor.authorde Araujo, Christiane Bezerrapt_BR
dc.contributor.authorLima, Loyze Paola Oliveira dept_BR
dc.contributor.authorCalderano, Simone Guedespt_BR
dc.contributor.authorDamasceno, Flávia Silvapt_BR
dc.contributor.authorSilbe, Ariel M.pt_BR
dc.contributor.authorElias, Maria Carolinapt_BR
dc.date.accessioned2020-07-09T21:23:56Z-
dc.date.available2020-07-09T21:23:56Z-
dc.date.issued2019pt_BR
dc.identifier.citationde Araujo CB, Lima LPO, Calderano SG, Damasceno FS, Silbe AM., Elias MC. Pep5, a fragment of Cyclin D2, shows antiparasitic effects in different stages of the Trypanosoma cruzi life cycle and blocks parasite infectivity. Antimicrob Agents Chemother. 2019 May;63(5):e01806-18. doi:10.1128/AAC.01806-18.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2746-
dc.description.abstractPep5 (WELVVLGKL) is a fragment of cyclin D2 that exhibits a 2-fold increase in the S phase of the HeLa cell cycle. When covalently bound to a cellpenetrating peptide (Pep5-cpp), the nonapeptide induces cell death in several tumor cells, including breast cancer and melanoma cells. Additionally, Pep5-cpp reduces the in vivo tumor volume of rat glioblastoma. Chagas disease, which is caused by the flagellated parasite Trypanosoma cruzi, is a neglected disease that occurs mainly in the Americas, where it is considered an important public health issue. Given that there are only two options for treating the disease, it is exceptionally crucial to search for new molecules with potential pharmacological action against the parasites. In this study, we demonstrate that Pep5-cpp induces cell death in epimastigote, trypomastigote, and amastigote forms of T. cruzi. The Pep5-cpp peptide was also able to decrease the percentage of infected cells without causing any detectable toxic effects in mammalian host cells. The infective, i.e., trypomastigote form of T. cruzi pretreated with Pep5-cpp was unable to infect LLC-MK2 monkey kidney cells. Also, Pep5-binding proteins were identified by mass spectrometry, including calmodulinubiquitin-associated protein, which is related to the virulence and parasitemia of T. cruzi. Taken together, these data suggest that Pep5 can be used as a novel alternative for the treatment of Chagas disease.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipGlobal Challenges Research Fund (GCRF)pt_BR
dc.format.extente01806-18pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofAntimicrobial Agents and Chemotherapypt_BR
dc.titlePep5, a fragment of Cyclin D2, shows antiparasitic effects in different stages of the Trypanosoma cruzi life cycle and blocks parasite infectivitypt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1128/AAC.01806-18pt_BR
dc.identifier.urlhttps://doi.org/10.1128/AAC.01806-18pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.identifier.citationvolume63pt_BR
dc.identifier.citationissue5pt_BR
dc.subject.keywordTrypanosoma cruzpt_BR
dc.subject.keywordcell deathpt_BR
dc.subject.keywordpeptide 5pt_BR
dc.relation.ispartofabbreviatedAntimicrob Agents Chemotherpt_BR
dc.identifier.citationabntv. 63, n. 5, e01806-18, mai. 2019pt_BR
dc.identifier.citationvancouver2019 May;63(5):e01806-18pt_BR
dc.contributor.butantanDe Araujo, Christiane Bezerra|:Aluno|:LCC - Laboratório de Ciclo Celular|:PrimeiroAutorpt_BR
dc.contributor.butantanLima, Loyze Paola Oliveira de|:Aluno|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanElias, Maria Carolina|:Pesquisador|:LCC - Laboratório de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.contributor.butantanCalderano, Simone Guedes|:Pesquisador|:Lab. Parasitologia|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦pt_BR
dc.sponsorship.butantanGlobal Challenges Research Fund (GCRF)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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