Biochemical and biological profile of parotoid secretion of the amazonian Rhinella marina (Anura: Bufonidae)

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dc.contributorLaboratório de Bioquímica e Biofísicapt_BR
dc.contributor.authorMedeiros, Daniel S. S. dept_BR
dc.contributor.authorRego, Tiago B.pt_BR
dc.contributor.authorSantos, Ana P. de A. dospt_BR
dc.contributor.authorPontes, Adriana S.pt_BR
dc.contributor.authorMoreira-Dill, Leandro S.pt_BR
dc.contributor.authorMatos, Najla B.pt_BR
dc.contributor.authorZuliani, Juliana Pavanpt_BR
dc.contributor.authorStábeli, Rodrigo G.pt_BR
dc.contributor.authorTeles, Carolina B.G.pt_BR
dc.contributor.authorSoares, Andreimar M.pt_BR
dc.contributor.authorSperotto, Angelo R. de M.pt_BR
dc.contributor.authorMoura, Dinara J.pt_BR
dc.contributor.authorSaffi, Jeniferpt_BR
dc.contributor.authorCaldeira, Cleópatra Alves da Silvapt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorCalderon, Leonardo A.pt_BR
dc.date.accessioned2020-07-09T21:24:05Z-
dc.date.available2020-07-09T21:24:05Z-
dc.date.issued2019-
dc.identifier.citationMedeiros DS.S., Rego TB., Santos AP.A., Pontes AS., Moreira-Dill LS., Matos NB., et al. Biochemical and biological profile of parotoid secretion of the amazonian Rhinella marina (Anura: Bufonidae). Biomed Res. Int.. 2019 Feb, 2019:2492315. doi:10.1155/2019/2492315.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2756-
dc.description.abstractSkin secretions of frogs have a high chemical complexity. They have diverse types of biomolecules, such as proteins, peptides, biogenic amines, and alkaloids. These compounds protect amphibians’ skin against growth of bacteria, fungi, and protozoa and participate in defense system against attack from predators. Therewith, this work performed biochemical and biological profile of macroglands parotoid secretion from cane toad. For poison analysis, we performed molecular exclusion and reverse phase chromatography, electrophoresis, and mass spectrometry. Antimicrobial, antiplasmodial, leishmanicidal, cytotoxicity, genotoxicity, and inflammatory activity of crude and/or fractions of R. marina secretion were also evaluated. Fractionation prior to filtration from poison showed separation of low mass content (steroids and alkaloids) and high molecular mass (protein). Material below 10 kDa two steroids, marinobufagin and desacetylcinobufagin, was detected. Crude extract and fractions were active against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Plasmodium falciparum, Leishmania guyanensis, and Leishmania braziliensis. Crude extract was also active against cancer cells although it was not cytotoxic for normal cells. This extract did not show significant DNA damage but it showed an important inflammatory effect in vivo. The information obtained in this work contributes to the understanding of the constituents of R. marina secretion as well as the bioactive potential of these molecules.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent2492315pt_BR
dc.languageengpt_BR
dc.relation.ispartofBioMed Research Internationalpt_BR
dc.titleBiochemical and biological profile of parotoid secretion of the amazonian Rhinella marina (Anura: Bufonidae)pt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1155/2019/2492315pt_BR
dc.identifier.urlhttps://doi.org/10.1155/2019/2492315pt_BR
dc.contributor.externalFundação Oswaldo Cruz de Rondônia (Fiocruz Rôndonia)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)¦¦Brasilpt_BR
dc.identifier.citationvolume2019pt_BR
dc.relation.ispartofabbreviatedBiomed Res. Int.pt_BR
dc.identifier.citationabntv. 2019, 2492315, fev. 2019pt_BR
dc.identifier.citationvancouver2019 Feb, 2019:2492315pt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Bioquímica e Biofísica|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.openairetypeArticle-
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item.grantfulltextembargo_29990101-
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