The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts

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dc.contributor(LDI) Laboratório de Desenvolvimento e Inovação Industrialpt_BR
dc.contributor.authorOlímpio, Regiane Marques Castropt_BR
dc.contributor.authorMoretto, Fernanda Cristina Fontespt_BR
dc.contributor.authorSibio, Maria Teresa Dept_BR
dc.contributor.authorOliveira, Miriane dept_BR
dc.contributor.authorMathias, Lucas Sollapt_BR
dc.contributor.authorGonçalves, Bianca Marianipt_BR
dc.contributor.authorDeprá, Igor Carvalhopt_BR
dc.contributor.authorTilli, Helena Paim.pt_BR
dc.contributor.authorRodrigues, Bruna Morettopt_BR
dc.contributor.authorSaraiva, Patrícia Pintopt_BR
dc.contributor.authorMaria, Durvanei Augustopt_BR
dc.contributor.authorNogueira, Célia Reginapt_BR
dc.date.accessioned2020-07-09T21:24:24Z-
dc.date.available2020-07-09T21:24:24Z-
dc.date.issued2019pt_BR
dc.identifier.citationOlímpio RMC, Moretto FCF, Sibio MTD, Oliveira M, Mathias LS, Gonçalves BM, et al. The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts. Life sci.. 2019 Aug;231:116556. doi:10.1016/j.lfs.2019.116556.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2780-
dc.description.abstractTriiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa ß (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E2 (10?nM), alone or in association with physiological (1nM) and supraphysiological (10nM) concentrations of T3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E2 enhanced the gene expression of OPG when associated with 1nM and 10nMT3. E2 was able to restore the bone matrix after an initial decrease using 1nM and 10nMT3. The protective effect of E2 on the RANKL and OPG signaling pathway was demonstrated. E2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T3 is in excess.pt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extent116556pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofLife Sciencespt_BR
dc.titleThe importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblastspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.lfs.2019.116556pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.lfs.2019.116556pt_BR
dc.contributor.external(UNESP) Universidade Estadual Paulista Júlio de Mesquita Filhopt_BR
dc.identifier.citationvolume231pt_BR
dc.subject.keywordStem cellspt_BR
dc.subject.keywordTriiodothyroninept_BR
dc.subject.keywordEstrogenpt_BR
dc.subject.keywordOsteoblastpt_BR
dc.subject.keywordRANKL and OPGpt_BR
dc.relation.ispartofabbreviatedLife scipt_BR
dc.identifier.citationabntv. 231, 116556, ago. 2019pt_BR
dc.identifier.citationvancouver2019 Aug;231:116556pt_BR
dc.contributor.butantanMaria, Durvanei Augusto|:Pesquisador|:Lab. Biologia Molecular|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/16406-9pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
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