
Experimental antivenom against serine proteases from the Bothrops jararaca venom obtained in mice, and its comparison with the antibothropic serum from the Butantan Institute
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | (LBI) Lab. Imunoquímica | pt_BR |
dc.contributor | (LETA) Lab. Toxinologia Aplicada | pt_BR |
dc.contributor.author | Kuniyoshi, Alexandre Kazuo | pt_BR |
dc.contributor.author | Kodama, Roberto Tadashi | pt_BR |
dc.contributor.author | Cajado-Carvalho, Daniela | pt_BR |
dc.contributor.author | Iwai, Leo Kei | pt_BR |
dc.contributor.author | Kitano, Eduardo Shigueo | pt_BR |
dc.contributor.author | Silva, Cristiane Castilho Fernandes da | pt_BR |
dc.contributor.author | Duzzi, Bruno | pt_BR |
dc.contributor.author | Silva, Wilmar Dias da | pt_BR |
dc.contributor.author | Portaro, Fernanda Calheta Vieira | pt_BR |
dc.date.accessioned | 2020-07-09T21:25:08Z | - |
dc.date.available | 2020-07-09T21:25:08Z | - |
dc.date.issued | 2019 | pt_BR |
dc.identifier.citation | Kuniyoshi AK, Kodama RT, Cajado-Carvalho D, Iwai LK, Kitano ES, Silva CCF, et al. Experimental antivenom against serine proteases from the Bothrops jararaca venom obtained in mice, and its comparison with the antibothropic serum from the Butantan Institute. Toxicon. 2019 Nov;169:59-67. doi:10.1016/j.toxicon.2019.09.001. | pt_BR |
dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/2835 | - |
dc.description.abstract | In Brazil, snakes from the Bothrops genus are responsible for thousands of accidents, and their venoms are mainly made up of proteolytic enzymes. Although the antibothropic serum produced by the Butantan Institute is remarkable in saving lives, studies show that some symptoms observed in cases of envenoming are not efficiently neutralized. Moreover, our group has shown that the commercial antivenom does not fully neutralize in vitro some serine proteases present in the Bothrops jararaca venom. Therefore, this study focuses on a new method in the production of specific immunoglobulins capable of neutralizing the activities of these enzymes in vitro. For this, a pool of serine proteases that was not inhibited by the commercial antivenom, made up of four enzymes (KN-BJ2, BjSP, HS112 and BPA) from the B. jararaca venom was obtained through two chromatographic steps (DEAE-HPLC and C8-RP-HPLC). The identities of these proteases were confirmed by SDS-PAGE, followed by tryptic digestion and mass spectrometry analysis. This pool was inoculated into BALB/c and C57BL/6 mice, using SBA-15 as adjuvant, and the produced IgGs were purified by affinity chromatography. The sera were characterized by ELISA, avidity and proteolytic neutralization assays. Both animal models responded to the immunization, producing higher IgGs titers when compared to the commercial antivenom. The experimental serum from BALB/c mice presented a better hydrolysis inhibition of the selective fluorescent substrate for serine proteases (~80%) when compared to C57BL/6 (~25%) and the commercial antivenom (<1%) at the dose of 500:1 (weight of antivenom:weight of venom). These results show that a different immunization method using isolated serine proteases improves the toxins neutralizing efficacy and could lead to a better end product to be used as a supplemental medicine to the currently used immunotherapy. | pt_BR |
dc.description.sponsorship | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo | pt_BR |
dc.description.sponsorship | (CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior | pt_BR |
dc.format.extent | 59-67 | pt_BR |
dc.language.iso | English | pt_BR |
dc.relation.ispartof | Toxicon | pt_BR |
dc.rights | Restricted access | pt_BR |
dc.title | Experimental antivenom against serine proteases from the Bothrops jararaca venom obtained in mice, and its comparison with the antibothropic serum from the Butantan Institute | pt_BR |
dc.type | Article | pt_BR |
dc.identifier.doi | 10.1016/j.toxicon.2019.09.001 | pt_BR |
dc.identifier.url | https://doi.org/10.1016/j.toxicon.2019.09.001 | pt_BR |
dc.identifier.citationvolume | 169 | pt_BR |
dc.subject.keyword | Bothrops jararaca | pt_BR |
dc.subject.keyword | serine protease | pt_BR |
dc.subject.keyword | venom | pt_BR |
dc.subject.keyword | antivenom | pt_BR |
dc.relation.ispartofabbreviated | Toxicon | pt_BR |
dc.identifier.citationabnt | v. 169, p. 59-67, nov. 2019 | pt_BR |
dc.identifier.citationvancouver | 2019 Nov;169:59-67 | pt_BR |
dc.contributor.butantan | Kuniyoshi, Alexandre Kazuo|:Aluno|:Lab. Imunoquímica|:PrimeiroAutor | pt_BR |
dc.contributor.butantan | Iwai, Leo Kei|:Pesquisador:Docente Colaborador PPGTOX|:(LETA) Lab. Toxinologia Aplicada|: | pt_BR |
dc.contributor.butantan | Portaro, Fernanda Calheta Vieira|:Pesquisador:Docente Permanente PPGTOX|:Lab. Imunoquímica|:Autor de correspondência | pt_BR |
dc.contributor.butantan | Kodama, Roberto Tadashi|:Aluno|:Lab. Imunoquímica|: | pt_BR |
dc.contributor.butantan | Cajado-Carvalho, Daniela|:Aluno|:Lab. Imunoquímica|: | pt_BR |
dc.contributor.butantan | Kitano, Eduardo Shigueo|:Aluno|:(LETA) Lab. Toxinologia Aplicada|: | pt_BR |
dc.contributor.butantan | Silva, Cristiane Castilho Fernandes da|:Aluno|:Lab. Imunoquímica|: | pt_BR |
dc.contributor.butantan | Duzzi, Bruno|:Aluno|:Lab. Imunoquímica|: | pt_BR |
dc.contributor.butantan | Silva, Wilmar Dias da|:Pesquisador|:Lab. Imunoquímica|: | pt_BR |
dc.sponsorship.butantan | (CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦ | pt_BR |
dc.sponsorship.butantan | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/15364-3 | pt_BR |
dc.sponsorship.butantan | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/15344-7 | pt_BR |
dc.sponsorship.butantan | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/13124-5 | pt_BR |
dc.sponsorship.butantan | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-1 | pt_BR |
dc.sponsorship.butantan | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2012/06677-00 | pt_BR |
dc.identifier.bvscc | BR78.1 | pt_BR |
dc.identifier.bvsdb | IBProd | pt_BR |
dc.subject.researchline | Bioprospecção e desenvolvimento | pt_BR |
dc.description.dbindexed | Yes | pt_BR |
item.openairetype | Article | - |
item.grantfulltext | embargo_29990101 | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | English | - |
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