Sa12b peptide from solitary wasp inhibits ASIC currents in rat dorsal root ganglion neurons

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dc.contributorLab. Genéticapt_BR
dc.contributor.authorHernández, Carmenpt_BR
dc.contributor.authorKonno, Katsuhiropt_BR
dc.contributor.authorSalceda, Emiliopt_BR
dc.contributor.authorVega, Rosariopt_BR
dc.contributor.authorZaharenko, Andre Junqueirapt_BR
dc.contributor.authorSoto, Enriquept_BR
dc.date.accessioned2020-07-09T21:25:34Z-
dc.date.available2020-07-09T21:25:34Z-
dc.date.issued2019pt_BR
dc.identifier.citationHernández C, Konno K, Salceda E, Vega R, Zaharenko AJ, Soto E. Sa12b peptide from solitary wasp inhibits ASIC currents in rat dorsal root ganglion neurons. Toxins. 2019 Oct;11(10):585. doi:10.3390/toxins11100585.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2867-
dc.description.abstractIn this work, we evaluate the effect of two peptides Sa12b (EDVDHVFLRF) and Sh5b (DVDHVFLRF-NH2) on Acid-Sensing Ion Channels (ASIC). These peptides were purified from the venom of solitary wasps Sphex argentatus argentatus and Isodontia harmandi, respectively. Voltage clamp recordings of ASIC currents were performed in whole cell configuration in primary culture of dorsal root ganglion (DRG) neurons from (P7-P10) CII Long-Evans rats. The peptides were applied by preincubation for 25 s (20 s in pH 7.4 solution and 5 s in pH 6.1 solution) or by co-application (5 s in pH 6.1 solution). Sa12b inhibits ASIC current with an IC50 of 81 nM, in a concentration-dependent manner when preincubation application was used. While Sh5b did not show consistent results having both excitatory and inhibitory effects on the maximum ASIC currents, its complex effect suggests that it presents a selective action on some ASIC subunits. Despite the similarity in their sequences, the action of these peptides differs significantly. Sa12b is the first discovered wasp peptide with a significant ASIC inhibitory effect.pt_BR
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología (CONACYT)pt_BR
dc.format.extent585pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen Accesspt_BR
dc.titleSa12b peptide from solitary wasp inhibits ASIC currents in rat dorsal root ganglion neuronspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3390/toxins11100585pt_BR
dc.identifier.urlhttps://doi.org/10.3390/toxins11100585pt_BR
dc.contributor.externalBenemérita Universidad Autónoma de Puebla (BUAP)¦¦Méxicopt_BR
dc.contributor.externalUniversity of Toyama¦¦Japãopt_BR
dc.identifier.citationvolume11pt_BR
dc.identifier.citationissue10pt_BR
dc.subject.keywordvenom peptidespt_BR
dc.subject.keywordFMRF-amidept_BR
dc.subject.keywordinsect neurotoxinpt_BR
dc.subject.keywordprotonspt_BR
dc.subject.keywordpH regulationpt_BR
dc.subject.keywordacid-sensing ion channelspt_BR
dc.subject.keywordacid-gated currentspt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 11, n. 10, p. 585, oct. 2019pt_BR
dc.identifier.citationvancouver2019 Oct;11(10):585pt_BR
dc.contributor.butantanZaharenko, Andre Junqueira|:Aluno|:Lab. Genética|:pt_BR
dc.sponsorship.butantanConsejo Nacional de Ciencia y Tecnología (CONACYT)¦¦669573pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1English-
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