Murine osteoclastogenesis suppression using conditioned media produced by melanoma or activated and non-activated jurkat-E6 cells

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dc.contributor(LG) Laboratório de Genéticapt_BR
dc.contributor.authorMambelli, Nicole Carolinept_BR
dc.contributor.authorFrare, Eduardo Osóriopt_BR
dc.contributor.authorCosta-Neves, Adrianapt_BR
dc.contributor.authorPrieto da Silva, Álvaro Rossan de Brandãopt_BR
dc.contributor.authorKerkis, Irinapt_BR
dc.identifier.citationMambelli NC, Frare EO, Neves AC, Prieto da Silva ARB, Kerkis I. Murine osteoclastogenesis suppression using conditioned media produced by melanoma or activated and non-activated jurkat-E6 cells. Cell Biol. Int.. 2020 Feb. doi:
dc.description.abstractConditioned medium (CM) (cell secretome) is a cocktail of growth factors, cytokines, and other soluble mediators secreted by cells into a culture medium. These growth factors are fundamental in many cellular processes such as cell growth, differentiation, and others and the composition of these factors is individual for each cell type. Osteoclasts are large multinucleated cells that are responsible for bone resorption. Immune and cancer cells are known to produce different growth factors, which are able to induce or inhibit osteoclast differentiation. Herein, we evaluated the effect of CM obtained from the supernatant of activated and non-activated Jukart-E6 cells, as well as from one murine (B16-F10) and one human melanoma cell line (SK­MEL­28). To induce osteoclast differentiation, murine bone marrow mononuclear cells were cultured in the presence and absence of differentiation factors (DF), such as macrophage colony-stimulating factor, prostaglandin E2, receptor activator of nuclear factor-capaB ligand, and CM. We measured the concentration of interleukin 6, tumor necrosis factor-a and interferon gama (IFN-gama) in CM that can inhibit or induce osteoclastogenesis. Our study demonstrated that CM obtained from each cell line suppresses or inhibits osteoclasts formation at early and intermediate stages of differentiation in the absence or presence of DF. CM obtained from activated Jurkat-E6 cells demonstrates a stronger effect when compared with CM from naïve Jurkat-E6 cells or human and murine melanoma cells. Moreover, CM obtained from activated Jurkat-E6 cells shows higher secretion of IFN-?, which is an inhibitor of osteoclastogenesis, in comparison with CM obtained from the three other cell lines. On the other hand, CM derived from B16-F10 cells showed a smaller inhibitory effect when compared with CM derived from the other cells.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.relation.ispartofCell Biology Internationalpt_BR
dc.titleMurine osteoclastogenesis suppression using conditioned media produced by melanoma or activated and non-activated jurkat-E6 cellspt_BR
dc.subject.keywordmurine osteoclastspt_BR
dc.relation.ispartofabbreviatedCell Biol Intpt_BR
dc.identifier.citationabntfev. 2020pt_BR
dc.identifier.citationvancouver2020 Febpt_BR
dc.contributor.butantanMambelli, Nicole Caroline|:Aluno|:Lab. Genética|:PrimeiroAutorpt_BR
dc.contributor.butantanKerkis, Irina|:Pesquisador:Docente Permanente PPGTOX|:Lab. Genética|:Autor de correspondênciapt_BR
dc.contributor.butantanFrare, Eduardo Osório|:Técnico|:Lab. Genética|:pt_BR
dc.contributor.butantanCosta-Neves, Adriana|:Pesquisador|:Lab. Genética|:pt_BR
dc.contributor.butantanPrieto da Silva, Álvaro Rossan de Brandão|:Pesquisador|:Lab. Genética|:pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/50040-4pt_BR
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