Behavioral and neurochemical characterization of the spontaneous mutation tremor, a new mouse model of audiogenic seizures

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dc.contributorLaboratório de Bioquímica e Biofísicapt_BR
dc.contributor.authorGarcia-Gomes, Mariana de Souza Aranhapt_BR
dc.contributor.authorZanatto, Dennis Albertpt_BR
dc.contributor.authorGalvis-Alonso, Orfa Yinethpt_BR
dc.contributor.authorMejia, Jorgept_BR
dc.contributor.authorAntiorio, Ana Tada Fonseca Brasilpt_BR
dc.contributor.authorYamamoto, Pedro Kenzopt_BR
dc.contributor.authorOlivato, Márcia Carolina Millánpt_BR
dc.contributor.authorSandini, Thaisa Meirapt_BR
dc.contributor.authorFlorio, Jorge Camilopt_BR
dc.contributor.authorLebrun, Ivopt_BR
dc.contributor.authorMassironi, Silvia Maria Gomespt_BR
dc.contributor.authorAlexandre-Ribeiro, Sandra Reginapt_BR
dc.contributor.authorBernardi, Maria Marthapt_BR
dc.contributor.authorIenne, Susanpt_BR
dc.contributor.authorSouza, Tiago Antonio dept_BR
dc.contributor.authorDagli, Maria Lúcia Zaidanpt_BR
dc.contributor.authorMori, Claudia Madalena Cabrerapt_BR
dc.identifier.citationGarcia-Gomes MSA, Zanatto DA, Galvis-Alonso OY, Mejia J, Antiorio ATFB, Yamamoto PK, et al. Behavioral and neurochemical characterization of the spontaneous mutation tremor, a new mouse model of audiogenic seizures. Epilepsy Behav.. 2020 Feb;105:106945. doi:10.1016/j.yebeh.2020.106945.pt_BR
dc.description.abstractThe tremor mutant phenotype results from an autosomal recessive spontaneous mutation arisen in a Swiss–Webster mouse colony. The mutant mice displayed normal development until three weeks of age when they began to present motor impairment comprised by whole body tremor, ataxia, and decreased exploratory behavior. These features increased in severity with aging suggesting a neurodegenerative profile. In parallel, they showed audiogenic generalized clonic seizures. Results from genetic mapping identified the mutation tremor on chromosome 14, in an interval of 5 cM between D14Mit37 (33.21cM) and D14Mit115 (38.21cM), making Early Growth Response 3 (Egr3) the main candidate gene. Comparing with wild type (WT) mice, the tremor mice showed higher hippocampal gene expression of Egr3 and Gabra1 and increased concentrations of noradrenalin (NOR; p=.0012), serotonin (5HT; p=.0083), 5-hydroxyindoleacetic acid (5-HIAA; p=.0032), gama-amino butyric acid (GABA; p=.0123), glutamate (p=.0217) and aspartate (p=.0124). In opposition, the content of glycine (p=.0168) and the vanillylmandelic acid (VMA)/NOR ratio (p=.032) were decreased. Regarding to dopaminergic system, neither dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) contents nor the turnover rate of DA showed statistically significant differences between WT and mutant mice. Data demonstrated that audiogenic seizures of tremor mice are associated with progressive motor impairment as well as to hippocampal alterations of the Egr3 and Gabra1 gene expression and amino acid and monoamine content. In addition, the tremor mice could be useful for study of neurotransmission pathways as modulators of epilepsy and the pathogenesis of epilepsies occurring with generalized clonic seizures.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.relation.ispartofEpilepsy & Behaviorpt_BR
dc.titleBehavioral and neurochemical characterization of the spontaneous mutation tremor, a new mouse model of audiogenic seizurespt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalFaculdade de Medicina de São José do Rio Preto (FAMERP)¦¦Brasilpt_BR
dc.contributor.externalHospital Israelita Albert Einstein¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Paulista (UNIP)¦¦Brasilpt_BR
dc.subject.keywordReflex seizurept_BR
dc.subject.keywordGenetic epilepsypt_BR
dc.subject.keywordBehavioral phenotypept_BR
dc.relation.ispartofabbreviatedEpilepsy Behav.pt_BR
dc.identifier.citationabntv. 105, 106945, fev. 2020pt_BR
dc.identifier.citationvancouver2020 Feb;105:106945pt_BR
dc.contributor.butantanLebrun, Ivo|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Bioquímica e Biofísica|:pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦001pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/21103-3pt_BR
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