Biogenic Aspergillus tubingensis silver nanoparticles’ in vitro effects on human umbilical vein endothelial cells, normal human fibroblasts, HEPG2, and Galleria mellonella

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dc.contributorLaboratório de Biologia Molecularpt_BR
dc.contributor.authorOttoni, Cristiane Angélicapt_BR
dc.contributor.authorMaria, Durvanei Augustopt_BR
dc.contributor.authorGonçalves, Priscila Jane Romano de Oliveirapt_BR
dc.contributor.authorAraújo, Welington Luiz dept_BR
dc.contributor.authorde Souza, Ana Olíviapt_BR
dc.date.accessioned2020-07-09T21:26:51Z-
dc.date.available2020-07-09T21:26:51Z-
dc.date.issued2019-
dc.identifier.citationOttoni CA, Maria DA, Gonçalves PJRO, Araújo WL, de Souza AO. Biogenic Aspergillus tubingensis silver nanoparticles’ in vitro effects on human umbilical vein endothelial cells, normal human fibroblasts, HEPG2, and Galleria mellonella. Toxicol. Res.. 2019 Nov;8(6):789–801. doi:10.1039/c9tx00091g.pt_BR
dc.identifier.issn2045-452X-
dc.identifier.issn2045-4538-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2969-
dc.description.abstractSilver nanoparticles (AgNPs) are widely incorporated into different hygiene, personal care, and healthcare products. However, few studies have been undertaken to determine the effects of biogenic AgNPs on human health. The effect of biosynthesized AgNPs using the fungus Aspergillus tubingensis culture was evaluated on human umbilical vein endothelial cells (HUVECs), normal human fibroblasts (FN1), human hepatoma cells (HEPG2) and a Galleria mellonella model. HUVECs were more susceptible to biogenic AgNPs than normal fibroblasts FN1 and intense cytotoxicity was observed only for very high concentrations at and above 2.5 µM for both cells. Normal human fibroblasts FN1 exposed to AgNPs for 24 h showed viability of 98.83 ± 8.40% and 94.86 ± 5.50% for 1.25 and 2.5 µM, respectively. At 5 and 10 µM, related to the control, an increase in cell viability was observed being 112.66 ± 9.94% and 117.86 ± 8.86%, respectively. Similar results were obtained for treatment for 48 and 72 h. At 1.25, 2.5, 5 and 10 µM of AgNPs, at 24 h, HUVECs showed 51.34 ± 7.47%, 27.01 ± 5.77%, 26.00 ± 3.03% and 27.64 ± 5.85% of viability, respectively. No alteration in cell distribution among different cycle phases was observed after HUVEC and normal fibroblast FN1 exposure to AgNPs from 0.01 to 1 µM for 24, 48 and 72 h. Based on the clonogenic assay, nanoparticles successfully inhibited HEPG2 cell proliferation when exposed to concentrations up to 1 µM. In addition to that, AgNPs did not induce senescence and no morphological alteration was observed by scanning electron microscopy on the endothelial cells. In the larvae of the wax moth, Galleria mellonella, a model for toxicity, AgNPs showed no significant effects, which corroborates to the safety of their use in mammalian cells. These results demonstrate that the use of A. tubingensis AgNPs is a promising biotechnological approach and these AgNPs can be applied in several biomedical situations.pt_BR
dc.format.extent789-801pt_BR
dc.languageengpt_BR
dc.publisherOxford University Presspt_BR
dc.relation.ispartofToxicology Researchpt_BR
dc.titleBiogenic Aspergillus tubingensis silver nanoparticles’ in vitro effects on human umbilical vein endothelial cells, normal human fibroblasts, HEPG2, and Galleria mellonellapt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1039/c9tx00091gpt_BR
dc.identifier.urlhttps://doi.org/10.1039/c9tx00091gpt_BR
dc.contributor.externalUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.publisher.cityOxfordpt_BR
dc.identifier.citationvolume8pt_BR
dc.identifier.citationissue6pt_BR
dc.relation.ispartofabbreviatedToxicol. Res.pt_BR
dc.identifier.citationabntv. 8, n. 6, p. 789–801, nov. 2019pt_BR
dc.identifier.citationvancouver2019 Nov;8(6):789–801pt_BR
dc.publisher.countryEnglandpt_BR
dc.contributor.butantanMaria, Durvanei Augusto|:Pesquisador|:Laboratório de Biologia Molecular|:pt_BR
dc.contributor.butantande Souza, Ana Olívia|:Pesquisador|:Laboratório de Biologia Molecular|:Autor de correspondênciapt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
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