Design of bioactive peptides derived from CART sequence isolated from the toadfish Thalassophryne nattereri

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dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorConceição, Katiapt_BR
dc.contributor.authorCena, Gabrielle L. dept_BR
dc.contributor.authorSilva, Verônica A. dapt_BR
dc.contributor.authorOliveira Neto, Xisto Antonio dept_BR
dc.contributor.authorAndrade, Vitor Martins dept_BR
dc.contributor.authorTada, Dayane Batistapt_BR
dc.contributor.authorRichardson, Michaelpt_BR
dc.contributor.authorChudzinski, Sonia Aparecida de Andradept_BR
dc.contributor.authorDias, Susana A.pt_BR
dc.contributor.authorCastanho, Miguel A. R. B.pt_BR
dc.contributor.authorLopes-Ferreira, Monicapt_BR
dc.identifier.citationConceição K, Cena GL., Silva VA., Oliveira Neto XA, Andrade VM, Tada DB, et al. Design of bioactive peptides derived from CART sequence isolated from the toadfish Thalassophryne nattereri. 3 Biotech. 2020 Mar;10:162. doi:10.1007/s13205-020-2151-4.pt_BR
dc.description.abstractThe emergence of bacterial resistance due to the indiscriminate use of antibiotics warrants the need for developing new bioactive agents. In this context, antimicrobial peptides are highly useful for managing resistant microbial strains. In this study, we report the isolation and characterization of peptides obtained from the venom of the toadfish Thalassophryne nattereri. These peptides were active against Gram-positive and Gram-negative bacteria and fungi. The primary amino acid sequences showed similarity to Cocaine and Amphetamine Regulated Transcript peptides, and two peptide analogs—Tn CRT2 and Tn CRT3—were designed using the AMPA algorithm based on these sequences. The analogs were subjected to physicochemical analysis and antimicrobial screening and were biologically active at concentrations ranging from 2.1 to 13 µM. Zeta potential analysis showed that the peptide analogs increased the positive charge on the cell surface of Gram-positive and Gram-negative bacteria. The toxicity of Tn CRT2 and Tn CRT3 were analyzed in vitro using a hemolytic assay and tetrazolium salt reduction in fibroblasts and was found to be significant only at high concentrations (up to 40 µM). These results suggest that this methodological approach is appropriate to design novel antimicrobial peptides to fight bacterial infections and represents a new and promising discovery in fish venom.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.relation.ispartof3 Biotechpt_BR
dc.titleDesign of bioactive peptides derived from CART sequence isolated from the toadfish Thalassophryne nattereript_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(FUNED) Fundação Ezequiel Diaspt_BR
dc.contributor.external(ULISBOA) Universidade de Lisboapt_BR
dc.subject.keywordThalassophryne nattereript_BR
dc.subject.keywordantimicrobial peptidept_BR
dc.relation.ispartofabbreviated3 Biotechpt_BR
dc.identifier.citationabntv. 10, 162, mar. 2020pt_BR
dc.identifier.citationvancouver2020 Mar;10:162pt_BR
dc.contributor.butantanChudzinski, Sonia Aparecida de Andrade|:Pesquisador:Docente Colaborador PPGTOX|:Laboratório de Toxinologia Aplicada|:pt_BR
dc.contributor.butantanLopes-Ferreira, Monica Valdyrce dos Anjos|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Toxinologia Aplicada|:pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/00032-0pt_BR
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