The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components

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dc.contributorLaboratório Especial de Desenvolvimento de Vacinas (LEDV)pt_BR
dc.contributorLaboratório de Bacteriologiapt_BR
dc.contributor.authorPassalia, Felipe Josépt_BR
dc.contributor.authorCarvalho, Eneaspt_BR
dc.contributor.authorHeinemann, Marcos Bryanpt_BR
dc.contributor.authorVieira, Mônica Laruccipt_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Oller dopt_BR
dc.date.accessioned2020-07-09T21:27:19Z-
dc.date.available2020-07-09T21:27:19Z-
dc.date.issued2020-
dc.identifier.citationPassalia FJ, Carvalho E, Heinemann MB, Vieira ML, Nascimento ALTO. The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components. Microbiol. Res.. 2020 May;235:126470. doi:10.1016/j.micres.2020.126470.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3001-
dc.description.abstractLeptospirosis is a global re-emerging zoonosis, caused by pathogenic bacteria of the genus Leptospira. Humans are infected mainly through contact with contaminated water or soil. The understanding of the molecular mechanisms of leptospirosis through the characterization of unknown outer membrane proteins may contribute to the development of new treatments, diagnostic methods and vaccines. We have identified using bioinformatics analysis a protein that is encoded by the gene LIC10774, predicted to be localized at the leptospiral outer membrane and exhibit beta-roll folding. Surface exposure was confirmed by flow cytometry, ELISA and immunofluorescence-based confocal microscopy. Through circular dichroism spectroscopy and hydrophobic dye binding we have shown that rLIC10774 binds calcium ions, which imposes changes to secondary and tertiary structures. The recombinant protein was capable of binding to several host extracellular matrix and serum components. Therefore, we describe LIC10774 as a calcium-binding protein exposed in the outer surface of pathogenic leptospires with possible multifunctional roles in adhesion to host tissues, evasion of the immune system and participation in dissemination processes during leptospirosis. In addition, we hypothesize that the calcium binding is important for temperature-dependent functional roles during leptospirosispt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent126470pt_BR
dc.languageengpt_BR
dc.relation.ispartofMicrobiological Researchpt_BR
dc.rightsOpen Accesspt_BR
dc.titleThe Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host componentspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.micres.2020.126470pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.micres.2020.126470pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de Minas Gerais (UFMG)¦¦Brasilpt_BR
dc.identifier.citationvolume235pt_BR
dc.subject.keywordLeptospirapt_BR
dc.subject.keywordLeptospirosispt_BR
dc.subject.keywordOuter-membrane-proteinspt_BR
dc.subject.keywordHost-pathogen-interactionspt_BR
dc.subject.keywordCalcium-binding proteinpt_BR
dc.relation.ispartofabbreviatedMicrobiol. Res.pt_BR
dc.identifier.citationabntv. 235, 126470, may. 2020pt_BR
dc.identifier.citationvancouver2020 May;235:126470pt_BR
dc.contributor.butantanPassalia, Felipe José|:Aluno|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)|:PrimeiroAutorpt_BR
dc.contributor.butantanCarvalho, Eneas|:Pesquisador|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanVieira, Mônica Larucci|:Aluno|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)|:Autor de correspondênciapt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦301229/2017-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/50981-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/00236-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/07054-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01102-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
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