The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components
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DC Field | Value | Language |
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dc.contributor | Laboratório Especial de Desenvolvimento de Vacinas (LEDV) | pt_BR |
dc.contributor | Laboratório de Bacteriologia | pt_BR |
dc.contributor.author | Passalia, Felipe José | pt_BR |
dc.contributor.author | Carvalho, Eneas | pt_BR |
dc.contributor.author | Heinemann, Marcos Bryan | pt_BR |
dc.contributor.author | Vieira, Mônica Larucci | pt_BR |
dc.contributor.author | Nascimento, Ana Lúcia Tabet Oller do | pt_BR |
dc.date.accessioned | 2020-07-09T21:27:19Z | - |
dc.date.available | 2020-07-09T21:27:19Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Passalia FJ, Carvalho E, Heinemann MB, Vieira ML, Nascimento ALTO. The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components. Microbiol. Res.. 2020 May;235:126470. doi:10.1016/j.micres.2020.126470. | pt_BR |
dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/3001 | - |
dc.description.abstract | Leptospirosis is a global re-emerging zoonosis, caused by pathogenic bacteria of the genus Leptospira. Humans are infected mainly through contact with contaminated water or soil. The understanding of the molecular mechanisms of leptospirosis through the characterization of unknown outer membrane proteins may contribute to the development of new treatments, diagnostic methods and vaccines. We have identified using bioinformatics analysis a protein that is encoded by the gene LIC10774, predicted to be localized at the leptospiral outer membrane and exhibit beta-roll folding. Surface exposure was confirmed by flow cytometry, ELISA and immunofluorescence-based confocal microscopy. Through circular dichroism spectroscopy and hydrophobic dye binding we have shown that rLIC10774 binds calcium ions, which imposes changes to secondary and tertiary structures. The recombinant protein was capable of binding to several host extracellular matrix and serum components. Therefore, we describe LIC10774 as a calcium-binding protein exposed in the outer surface of pathogenic leptospires with possible multifunctional roles in adhesion to host tissues, evasion of the immune system and participation in dissemination processes during leptospirosis. In addition, we hypothesize that the calcium binding is important for temperature-dependent functional roles during leptospirosis | pt_BR |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | pt_BR |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | pt_BR |
dc.format.extent | 126470 | pt_BR |
dc.language | eng | pt_BR |
dc.relation.ispartof | Microbiological Research | pt_BR |
dc.rights | Open Access | pt_BR |
dc.title | The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components | pt_BR |
dc.type | Article | pt_BR |
dc.identifier.doi | 10.1016/j.micres.2020.126470 | pt_BR |
dc.identifier.url | https://doi.org/10.1016/j.micres.2020.126470 | pt_BR |
dc.contributor.external | Universidade de São Paulo (USP)¦¦Brasil | pt_BR |
dc.contributor.external | Universidade Federal de Minas Gerais (UFMG)¦¦Brasil | pt_BR |
dc.identifier.citationvolume | 235 | pt_BR |
dc.subject.keyword | Leptospira | pt_BR |
dc.subject.keyword | Leptospirosis | pt_BR |
dc.subject.keyword | Outer-membrane-proteins | pt_BR |
dc.subject.keyword | Host-pathogen-interactions | pt_BR |
dc.subject.keyword | Calcium-binding protein | pt_BR |
dc.relation.ispartofabbreviated | Microbiol. Res. | pt_BR |
dc.identifier.citationabnt | v. 235, 126470, may. 2020 | pt_BR |
dc.identifier.citationvancouver | 2020 May;235:126470 | pt_BR |
dc.contributor.butantan | Passalia, Felipe José|:Aluno|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)|:PrimeiroAutor | pt_BR |
dc.contributor.butantan | Carvalho, Eneas|:Pesquisador|:Laboratório de Bacteriologia|: | pt_BR |
dc.contributor.butantan | Vieira, Mônica Larucci|:Aluno|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)|:Autor de correspondência | pt_BR |
dc.contributor.butantan | Nascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)|:Autor de correspondência | pt_BR |
dc.sponsorship.butantan | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦301229/2017-1 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/50981-0 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/00236-5 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/07054-2 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01102-2 | pt_BR |
dc.identifier.bvscc | BR78.1 | pt_BR |
dc.identifier.bvsdb | IBProd | pt_BR |
item.openairetype | Article | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | embargo_29990101 | - |
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