Zebrafish embryo sensitivity test as in vivo platform to anti-Shiga toxin compound screening

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dc.contributorLab. Bacteriologiapt_BR
dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorMelo, Bruna de Sousapt_BR
dc.contributor.authorFernandes, Bianca Helena Venturapt_BR
dc.contributor.authorLopes-Ferreira, Monicapt_BR
dc.contributor.authorHenrique, Camilapt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.contributor.authorLuz, Danielapt_BR
dc.date.accessioned2020-07-09T21:27:51Z-
dc.date.available2020-07-09T21:27:51Z-
dc.date.issued2020pt_BR
dc.identifier.citationMelo BS, Fernandes BHV, Lopes-Ferreira M, Henrique C, Piazza RMF, Luz D. Zebrafish embryo sensitivity test as in vivo platform to anti-Shiga toxin compound screening. Braz. J. Microbiol.. 2020 May;51:1021-1027. doi:10.1007/s42770-020-00305-1.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3046-
dc.description.abstractShiga toxin-producing Escherichia coli (STEC) pathotype secretes two types of AB5 cytotoxins (Stx1 and Stx2), responsible for complications such as hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS) in infected patients, which could lead to sequels and death. Currently, there is no effective treatment against the cytotoxic effect of these toxins. However, in order to approve any therapy molecule, an animal experiment is required in order to evaluate the efficacy and safety of therapeutic approaches. The use of alternative small host models is growing among human infectious disease studies, particularly the vertebrate zebrafish model, since relevant results have been described for pathogen-host interaction. In this sense, the present work aimed to analyze the toxic effect of Shiga toxins in zebrafish embryo model in order to standardize this method in the future to be used as a fast, simple, and efficient methodology for the screening of therapeutic molecules. Herein, we demonstrated that the embryos were sensitive in a dose-dependent manner to both Stx toxins, with LD50 of 22 µg/mL for Stx1 and 33 µg/mL for Stx2, and the use of anti-Stx polyclonal antibody abolished the toxic effect. Therefore, this methodology can be a rapid alternative method for selecting promising compounds against Stx toxins, such as recombinant antibodies.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent1021-1027pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofBrazilian Journal of Microbiologypt_BR
dc.titleZebrafish embryo sensitivity test as in vivo platform to anti-Shiga toxin compound screeningpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1007/s42770-020-00305-1pt_BR
dc.identifier.urlhttps://doi.org/10.1007/s42770-020-00305-1pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.identifier.citationvolume51pt_BR
dc.subject.keywordZebrafishpt_BR
dc.subject.keywordShiga toxinpt_BR
dc.subject.keywordToxicitypt_BR
dc.subject.keywordSTECpt_BR
dc.relation.ispartofabbreviatedBraz J Microbiolpt_BR
dc.identifier.citationabntv. 51, p. 1021-1027, mai. 2020pt_BR
dc.identifier.citationvancouver2020 May;51:1021-1027pt_BR
dc.contributor.butantanMelo, Bruna de Sousa|:Aluno|:Lab. Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanLopes-Ferreira, Monica Valdyrce dos Anjos|:Pesquisador:Docente Permanente PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanHenrique, Camila|:Aluno|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanLuz, Daniela|:Aluno|:Lab. Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦301302/2013-8pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦PNPD20132071pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2011/12928-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/17006-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextSem Texto completo-
item.grantfulltextnone-
item.languageiso639-1English-
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