Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina

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dc.contributorLab. Bioquímicapt_BR
dc.contributor.authorFusco, Luciano S.pt_BR
dc.contributor.authorBeraldo Neto, Emidiopt_BR
dc.contributor.authorFrancisco, Aleff F.pt_BR
dc.contributor.authorAlfonso, Jorgept_BR
dc.contributor.authorSoares, Andreimarpt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorLeiva, Laura C.pt_BR
dc.date.accessioned2020-07-30T13:56:55Z-
dc.date.available2020-07-30T13:56:55Z-
dc.date.issued2020pt_BR
dc.identifier.citationFusco LS., Beraldo Neto E, Francisco AF., Alfonso J, Soares A, Pimenta DC, et al. Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina. Toxicon X. 2020 set;7:100047. doi:10.1016/j.toxcx.2020.100047.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3086-
dc.description.abstractThe complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFondo para la Investigación Científica y Tecnológica (FONCyT)pt_BR
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)pt_BR
dc.description.sponsorshipFundação Rondônia de Amparo ao Desenvolvimento das Ações Científicas e Tecnológicas e à Pesquisa do Estado de Rondônia (Fapero)pt_BR
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología (CONACYT)pt_BR
dc.format.extent100047pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxicon Xpt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/pt_BR
dc.titleFast venomic analysis of Crotalus durissus terrificus from northeastern Argentinapt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BY-NC-NDpt_BR
dc.identifier.doi10.1016/j.toxcx.2020.100047pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.toxcx.2020.100047pt_BR
dc.contributor.externalUniversidad Nacional del Nordeste (UNNE)pt_BR
dc.contributor.externalFundação Oswaldo Cruz (Fiocruz)pt_BR
dc.contributor.externalUniversidade Federal de Rondônia (UNIR)pt_BR
dc.contributor.externalCentro Para el Desarrollo de la Investigación Científica (CEDIC)pt_BR
dc.contributor.externalCentro Universitário São Lucas (UNISL)pt_BR
dc.contributor.externalUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP)pt_BR
dc.identifier.citationvolume7pt_BR
dc.relation.ispartofabbreviatedToxicon Xpt_BR
dc.identifier.citationabntv. 7, 100047, set. 2020pt_BR
dc.identifier.citationvancouver2020 set;7:100047pt_BR
dc.contributor.butantanBeraldo Neto, Emidio|:Aluno|:Lab. Bioquímicapt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bioquímicapt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦001pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦#01.09.0278.04pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦#01.12.0450.03pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)¦¦406385/2018-1pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)¦¦303792/2016-7pt_BR
dc.sponsorship.butantanFondo para la Investigación Científica y Tecnológica (FONCyT)pt_BR
dc.sponsorship.butantanConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)pt_BR
dc.sponsorship.butantanFundação Rondônia de Amparo ao Desenvolvimento das Ações Científicas e Tecnológicas e à Pesquisa do Estado de Rondônia (Fapero)pt_BR
dc.sponsorship.butantanConsejo Nacional de Ciencia y Tecnología (CONACYT)pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
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