Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications: study of changes in epigenetic marks through the parasite's metacyclogenesis and life cycle

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dc.contributorLaboratório Especial de Ciclo Celularpt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorLima, Loyze Paola Oliveira dept_BR
dc.contributor.authorPoubel, Saloê Bispopt_BR
dc.contributor.authorYuan, Zuo-Feipt_BR
dc.contributor.authorRosón, Juliana Nunespt_BR
dc.contributor.authorVitorino, Francisca Nathália de Lunapt_BR
dc.contributor.authorHoletz, Fabiola Barbieript_BR
dc.contributor.authorGarcia, Benjamin A.pt_BR
dc.contributor.authorda Cunha, Julia Pinheiro Chagaspt_BR
dc.date.accessioned2020-08-12T14:15:56Z-
dc.date.available2020-08-12T14:15:56Z-
dc.date.issued2020-
dc.identifier.citationLima LPO, Poubel SB, Yuan Z-F, Rosón JN, Vitorino FNL, Holetz FB, et al. Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications: study of changes in epigenetic marks through the parasite's metacyclogenesis and life cycle. J. Proteomics. 2020 Aug;225:103847. doi:10.1016/j.jprot.2020.103847.pt_BR
dc.identifier.issn1874-3919-
dc.identifier.issn1876-7737-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3126-
dc.description.abstractTrypanosome histone N-terminal sequences are very divergent from the other eukaryotes, although they are still decorated by post-translational modifications (PTMs). Here, we used a highly robust workflow to analyze histone PTMs in the parasite Trypanosoma cruzi using mass spectrometry-based (MS-based) data-independent acquisition (DIA). We adapted the workflow for the analysis of the parasite's histone sequences by modifying the software EpiProfile 2.0, improving peptide and PTM quantification accuracy. This workflow could now be applied to the study of 141 T. cruzi modified histone peptides, which we used to investigate the dynamics of histone PTMs along the metacyclogenesis and the life cycle of T. cruzi. Global levels of histone acetylation and methylation fluctuates along metacyclogenesis, however most critical differences were observed between parasite life forms. More than 66 histone PTM changes were detected. Strikingly, the histone PTM pattern of metacyclic trypomastigotes is more similar to epimastigotes than to cellular trypomastigotes. Finally, we highlighted changes at the H4 N-terminus and at H3K76 discussing their impact on the trypanosome biology. Altogether, we have optimized a workflow easily applicable to the analysis of histone PTMs in T. cruzi and generated a dataset that may shed lights on the role of chromatin modifications in this parasite.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipInstituto Serrapilheirapt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent103847pt_BR
dc.languageengpt_BR
dc.publisherElsevierpt_BR
dc.relation.ispartofJournal of Proteomicspt_BR
dc.titleImprovements on the quantitative analysis of Trypanosoma cruzi histone post translational modificationspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.jprot.2020.103847pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.jprot.2020.103847pt_BR
dc.contributor.externalUniversity of Pennsylvaniapt_BR
dc.contributor.externalInstituto Carlos Chagas (ICC). Fundação Oswaldo Cruz (FioCruz)pt_BR
dc.title.substudy of changes in epigenetic marks through the parasite's metacyclogenesis and life cyclept_BR
dc.publisher.cityAmsterdampt_BR
dc.identifier.citationvolume225pt_BR
dc.subject.keywordHistone PTMpt_BR
dc.subject.keywordMetacyclogenesispt_BR
dc.subject.keywordTrypanosoma cruzipt_BR
dc.subject.keywordLife cyclept_BR
dc.subject.keywordEpiProfilept_BR
dc.relation.ispartofabbreviatedJ. Proteomicspt_BR
dc.identifier.citationabntv. 225, 103847, ago. 2020pt_BR
dc.identifier.citationvancouver2020 Aug;225:103847pt_BR
dc.publisher.countryNetherlandspt_BR
dc.contributor.butantanLima, Loyze Paola Oliveira de|:Aluno|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:PrimeiroAutorpt_BR
dc.contributor.butantanPoubel, Saloê Bispo|:Aluno|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanRoson, Juliana Nunes|:Aluno|:Laboratório Especial de Ciclo Celular|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanVitorino, Francisca Nathália de Luna|:Aluno|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanda Cunha, Julia Pinheiro Chagas|:Pesquisador|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦18/15553-9pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦17/18344-9pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦17/06104-3pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦18/14432-3pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦18/21785-0pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦13/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦pt_BR
dc.sponsorship.butantanInstituto Serrapilheira¦¦1709-1686pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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