Bothrops jararaca venom proteome rearrangement upon neonate to adult transition

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dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributorLab. Herpetologiapt_BR
dc.contributorCentro Bioindustrialpt_BR
dc.contributor.authorPereira, André Zelanis Palitotpt_BR
dc.contributor.authorTashima, Alexandre Keijipt_BR
dc.contributor.authorPinto, Antônio F. M.pt_BR
dc.contributor.authorStuginski, Daniel Rodriguespt_BR
dc.contributor.authorFurtado, Maria de Fatima Dominguespt_BR
dc.contributor.authorSherman, Nicholas E.pt_BR
dc.contributor.authorHo, Paulo Leept_BR
dc.contributor.authorFox, Jay W.pt_BR
dc.contributor.authorSerrano, Solange Maria de Toledopt_BR
dc.identifier.citationPereira AZP, Tashima AK, Pinto AF.M., Stuginski DR, Furtado MFD, Sherman NE., et al. Bothrops jararaca venom proteome rearrangement upon neonate to adult transition. Proteomics. 2011 Nov;11(21):4218-4228. doi:10.1002/pmic.201100287.pt_BR
dc.description.abstractThe pharmacological activities displayed by Bothrops jararaca venom undergo a significant ontogenetic shift. Similarly, the diet of this species changes from ectothermic prey in early life to endothermic prey in adulthood. In this study we used large and representative newborn and adult venom samples consisting of pools from 694 and 110 specimens, respectively, and demonstrate a significant ontogenetic shift in the venom proteome complexity of B. jararaca. 2‐DE coupled to MS protein identification showed a clear rearrangement of the toxin arsenal both in terms of the total proteome, as of the glycoproteome. N‐glycosylation seems to play a key role in venom protein variability between newborn and adult specimens. Upon the snake development, the subproteome of metalloproteinases undergoes a shift from a P‐III‐rich to a P‐I‐rich profile while the serine proteinase profile does not vary significantly. We also used isobaric tag labeling (iTRAQ) of venom tryptic peptides for the first time to examine the quantitative changes in the venom toxins of B. jararaca upon neonate to adult transition. The iTRAQ analysis showed changes in various toxin classes, especially the proteinases. Our study expands the in‐depth understanding of venom complexity variation particularly with regard to toxin families that have been associated with envenomation pathogenesis.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extentp. 4218-4228pt_BR
dc.rightsRestricted Accesspt_BR
dc.titleBothrops jararaca venom proteome rearrangement upon neonate to adult transitionpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)pt_BR
dc.contributor.externalUniversidade Federal do Rio Grande do Sul (UFRGS)pt_BR
dc.contributor.externalLaboratório Nacional de Biociências (LNBio)pt_BR
dc.contributor.externalUniversity of Virginia (UVA)pt_BR
dc.subject.keywordontogenetic venom variationpt_BR
dc.subject.keywordsnake venompt_BR
dc.identifier.citationabntv. 11, n. 21, p. 4218-4228, nov. 2011pt_BR
dc.identifier.citationvancouver2011 Nov;11(21):4218-4228pt_BR
dc.contributor.butantanPereira, André Zelanis Palitot|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:PrimeiroAutorpt_BR
dc.contributor.butantanTashima, Alexandre Keiji|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributor.butantanStuginski, Daniel Rodrigues|:Técnico|:Lab. Herpetologiapt_BR
dc.contributor.butantanFurtado, Maria de Fatima Domingues|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanHo, Paulo Lee|:Pesquisador|:Centro Bioindustrialpt_BR
dc.contributor.butantanSerrano, Solange Maria de Toledo|:Pesquisador:Docente permanente PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦07/54626‐7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦98/14307‐9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2004/14846‐0pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦001pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦01.07.0290.00pt_BR
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