A new class of antimicrobial molecules derived from kefir, effective against Pseudomonas aeruginosa and methicillin resistant Staphylococcus aureus (MRSA) strains

Full metadata record
DC FieldValueLanguage
dc.contributorLab. Bacteriologiapt_BR
dc.contributorLab. Biofísicapt_BR
dc.contributorLab. Biotecnologia Viralpt_BR
dc.contributor.authorMarques, Vaniky Duartept_BR
dc.contributor.authorFranzolin, Marcia Reginapt_BR
dc.contributor.authorSanabani, Sabri Saeedpt_BR
dc.contributor.authorVigerelli, Hugopt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorVenâncio, Tiagopt_BR
dc.contributor.authorNeves, Irys Vianapt_BR
dc.contributor.authorSIlva, Herbert Guimarães de Souzapt_BR
dc.contributor.authorCourrol, Daniella dos Santospt_BR
dc.contributor.authorMendonça-Previato, Luciapt_BR
dc.contributor.authorPreviato, José Osvaldopt_BR
dc.contributor.authorJorge, Soraia Attie Calilpt_BR
dc.contributor.authorDomingos, Marta De Oliveirapt_BR
dc.date.accessioned2020-10-26T18:12:46Z-
dc.date.available2020-10-26T18:12:46Z-
dc.date.issued2020pt_BR
dc.identifier.citationMarques VD, Franzolin MR, Sanabani SS, Vigerelli H, Piazza RMF, Pimenta DC, et al. A new class of antimicrobial molecules derived from kefir, effective against Pseudomonas aeruginosa and methicillin resistant Staphylococcus aureus (MRSA) strains. Sci. Rep.. 2020 Oct;10:17434. doi:10.1038/s41598-020-73651-7.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3283-
dc.description.abstractMany studies have linked the antimicrobial properties of kefir with the presence of bacteriocins and organic acids. In the present work, results obtained from bacteriostatic and bactericidal studies, and from RP-HPLC, Mass Spectrometry and proton NMR analysis, show that a sample of milk kefir grains is able to produce an antimicrobial fraction, denoted FK-1000, composed of sugars and amino acids, predominantly polymers of alanine, doublets of tyrosine and phenylalanine. Since this fraction is a lyophilized product whose molecular profile is different from bacteriocins and simple carboxylic acids, its antimicrobial effect cannot be attributed to these molecules, or to alcohols or hydrogen peroxide. The fraction is bactericidal against weak-acid-resistant MRSA and weak-acid resistant P. aeruginosa at pH 5, and is bacteriostatic against both pathogens at pH 7. In combination formulation, the FK-1000 fraction is able to increase fivefold the effect of streptomycin against P. aeruginosa and it is not toxic to human epithelial cells at antimicrobial concentrations. 16 S rRNA microbiota analysis of antimicrobial-producing and non-producing kefir grains demonstrated that they are distinct. In summary, the results indicate that milk kefir grains can produce different classes of molecules with potent antibiotic activity against resistant bacteria.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extent17434pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofScientific Reportspt_BR
dc.rightsOpen Accesspt_BR
dc.titleA new class of antimicrobial molecules derived from kefir, effective against Pseudomonas aeruginosa and methicillin resistant Staphylococcus aureus (MRSA) strainspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1038/s41598-020-73651-7pt_BR
dc.identifier.urlhttps://doi.org/10.1038/s41598-020-73651-7pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalUniversidade Federal de São Carlos (UFSCar)pt_BR
dc.contributor.externalUniversidade Federal do Rio de Janeiro (UFRJ)pt_BR
dc.identifier.citationvolume10pt_BR
dc.subject.keyworddrug discoverypt_BR
dc.subject.keywordMicrobiologypt_BR
dc.relation.ispartofabbreviatedSci Reppt_BR
dc.identifier.citationabntv. 10, 17434, out. 2020pt_BR
dc.identifier.citationvancouver2020 Oct;10:17434pt_BR
dc.contributor.butantanMarques, Vaniky Duarte|:Aluno|:Lab. Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanFranzolin, Marcia Regina|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.contributor.butantanVigerelli, Hugo|:Aluno:Docente colaborador PPGTOX |:Lab. Biofísicapt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador:Docente permanente PPGTOX|:Lab. Bacteriologiapt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente permanente PPGTOX|:Lab. Biofísicapt_BR
dc.contributor.butantanNeves, Irys Viana|:Aluno|:Lab. Bacteriologiapt_BR
dc.contributor.butantanSIlva, Herbert Guimarães de Souza|:Aluno|:Lab. Bacteriologiapt_BR
dc.contributor.butantanCourrol, Daniella dos Santos|:Aluno|:Lab. Bacteriologiapt_BR
dc.contributor.butantanJorge, Soraia Attie Calil|:Pesquisador|:Lab. Biotecnologia Viralpt_BR
dc.contributor.butantanDomingos, Marta de Oliveira|:Lab. Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦406385/2018-1pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦303792/2016-7pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦01.09.0278.04pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦01.12.0450.03pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1English-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-0654-639X-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-8197-2260-
crisitem.author.orcid0000-0003-0068-8506-
crisitem.author.orcid0000-0003-2406-0860-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0001-9380-1023-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

10.1038s41598-020-73651-7.pdf
Description:
Size: 3.44 MB
Format: Adobe PDF
View/Open
Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.