Identification of novel interspersed DNA repetitive elements in the Trypanosoma cruzi genome associated with the 3′UTRs of surface multigenic families
Appears in Collections:
Trypanosoma cruzi is the etiological agent of Chagas disease, which affects millions of people in Latin America. No transcriptional control of gene expression has been demonstrated in this organism, and 50% of its genome consists of repetitive elements and members of multigenic families. In this study, we applied a novel bioinformatics approach to predict new repetitive elements in the genome sequence of T. cruzi. A new repetitive sequence measuring 241 nt was identified and found to be interspersed along the genome sequence from strains of different DTUs. This new repeat was mostly on intergenic regions, and upstream and downstream regions of the 241 nt repeat were enriched in surface protein genes. RNAseq analysis revealed that the repeat was part of processed mRNAs and was predominantly found in the 3′ untranslated regions (UTRs) of genes of multigenic families encoding surface proteins. Moreover, we detected a correlation between the presence of the repeat in the 3′UTR of multigenic family genes and the level of differential expression of these genes when comparing epimastigote and trypomastigote transcriptomes. These data suggest that this sequence plays a role in the posttranscriptional regulation of the expression of multigenic families.
Calderano SG, Nishiyama-Junior MY, Marini M, Nunes NO, Reis MS, Patané JSL, et al. Identification of novel interspersed DNA repetitive elements in the Trypanosoma cruzi genome associated with the 3′UTRs of surface multigenic families. Genes. 2020 Oct;11(10):1235. doi:10.3390/genes11101235.
Link to cite this reference
The access to the publications deposited in this repository respects the licenses from journals and publishers.