Identification of novel interspersed DNA repetitive elements in the Trypanosoma cruzi genome associated with the 3′UTRs of surface multigenic families

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dc.contributorLab. Parasitologiapt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributorLCC - Laboratório de Ciclo Celularpt_BR
dc.contributor.authorCalderano, Simone Guedespt_BR
dc.contributor.authorNishiyama Junior, Milton Yutakapt_BR
dc.contributor.authorMarini, Marjoriept_BR
dc.contributor.authorNunes, Nathan de Oliveirapt_BR
dc.contributor.authorReis, Marcelo da Silvapt_BR
dc.contributor.authorPatané, José Salvatore Leisterpt_BR
dc.contributor.authorSilveira, José Franco dapt_BR
dc.contributor.authorCunha, Julia Pinheiro Chagas dapt_BR
dc.contributor.authorElias, Maria Carolinapt_BR
dc.date.accessioned2020-10-28T14:12:43Z-
dc.date.available2020-10-28T14:12:43Z-
dc.date.issued2020pt_BR
dc.identifier.citationCalderano SG, Nishiyama-Junior MY, Marini M, Nunes NO, Reis MS, Patané JSL, et al. Identification of novel interspersed DNA repetitive elements in the Trypanosoma cruzi genome associated with the 3′UTRs of surface multigenic families. Genes. 2020 Oct;11(10):1235. doi:10.3390/genes11101235.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3290-
dc.description.abstractTrypanosoma cruzi is the etiological agent of Chagas disease, which affects millions of people in Latin America. No transcriptional control of gene expression has been demonstrated in this organism, and 50% of its genome consists of repetitive elements and members of multigenic families. In this study, we applied a novel bioinformatics approach to predict new repetitive elements in the genome sequence of T. cruzi. A new repetitive sequence measuring 241 nt was identified and found to be interspersed along the genome sequence from strains of different DTUs. This new repeat was mostly on intergenic regions, and upstream and downstream regions of the 241 nt repeat were enriched in surface protein genes. RNAseq analysis revealed that the repeat was part of processed mRNAs and was predominantly found in the 3′ untranslated regions (UTRs) of genes of multigenic families encoding surface proteins. Moreover, we detected a correlation between the presence of the repeat in the 3′UTR of multigenic family genes and the level of differential expression of these genes when comparing epimastigote and trypomastigote transcriptomes. These data suggest that this sequence plays a role in the posttranscriptional regulation of the expression of multigenic families.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent1235pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofGenespt_BR
dc.rightsOpen Accesspt_BR
dc.titleIdentification of novel interspersed DNA repetitive elements in the Trypanosoma cruzi genome associated with the 3′UTRs of surface multigenic familiespt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3390/genes11101235pt_BR
dc.identifier.urlhttps://doi.org/10.3390/genes11101235pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)pt_BR
dc.contributor.externalCentro Universitário São Camilopt_BR
dc.identifier.citationvolume11pt_BR
dc.identifier.citationissue10pt_BR
dc.subject.keywordTrypanosoma cruzipt_BR
dc.subject.keywordgenomept_BR
dc.subject.keywordrepeatspt_BR
dc.subject.keyword3′UTRpt_BR
dc.subject.keywordmultigenic familypt_BR
dc.relation.ispartofabbreviatedGenespt_BR
dc.identifier.citationabntv. 11, n. 10, 1235, out. 2020pt_BR
dc.identifier.citationvancouver2020 Oct;11(10):1235pt_BR
dc.contributor.butantanCalderano, Simone Guedes|:Pesquisador|:Lab. Parasitologia:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:PrimeiroAutorpt_BR
dc.contributor.butantanNishiyama Junior, Milton Yutaka|:Pesquisador|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):Laboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributor.butantanNunes, Nathan de Oliveira|:Aluno|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):Laboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributor.butantanReis, Marcelo da Silva|:Pesquisador|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):LCC - Laboratório de Ciclo Celularpt_BR
dc.contributor.butantanPatané, José Salvatore Leister|:Técnico|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):LCC - Laboratório de Ciclo Celularpt_BR
dc.contributor.butantanDa Cunha, Julia Pinheiro Chagas|:Pesquisador|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):LCC - Laboratório de Ciclo Celularpt_BR
dc.contributor.butantanElias, Maria Carolina|:Pesquisador|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):LCC - Laboratório de Ciclo Celular|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/50050-2pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦306199/2018-1pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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