Identification of novel interspersed DNA repetitive elements in the Trypanosoma cruzi genome associated with the 3′UTRs of surface multigenic families
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | Laboratório de Parasitologia | pt_BR |
dc.contributor | Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS) | pt_BR |
dc.contributor | Laboratório Especial de Toxinologia Aplicada (LETA) | pt_BR |
dc.contributor | Laboratório Especial de Ciclo Celular | pt_BR |
dc.contributor.author | Calderano, Simone Guedes | pt_BR |
dc.contributor.author | Nishiyama-Junior, Milton Yutaka | pt_BR |
dc.contributor.author | Marini, Marjorie | pt_BR |
dc.contributor.author | Nunes, Nathan de Oliveira | pt_BR |
dc.contributor.author | Reis, Marcelo da Silva | pt_BR |
dc.contributor.author | Patané, José Salvatore Leister | pt_BR |
dc.contributor.author | Silveira, José Franco da | pt_BR |
dc.contributor.author | Cunha, Julia Pinheiro Chagas da | pt_BR |
dc.contributor.author | Elias, Maria Carolina | pt_BR |
dc.date.accessioned | 2020-10-28T14:12:43Z | - |
dc.date.available | 2020-10-28T14:12:43Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Calderano SG, Nishiyama-Junior MY, Marini M, Nunes NO, Reis MS, Patané JSL, et al. Identification of novel interspersed DNA repetitive elements in the Trypanosoma cruzi genome associated with the 3′UTRs of surface multigenic families. Genes. 2020 Oct;11(10):1235. doi:10.3390/genes11101235. | pt_BR |
dc.identifier.issn | 2073-4425 | - |
dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/3290 | - |
dc.description.abstract | Trypanosoma cruzi is the etiological agent of Chagas disease, which affects millions of people in Latin America. No transcriptional control of gene expression has been demonstrated in this organism, and 50% of its genome consists of repetitive elements and members of multigenic families. In this study, we applied a novel bioinformatics approach to predict new repetitive elements in the genome sequence of T. cruzi. A new repetitive sequence measuring 241 nt was identified and found to be interspersed along the genome sequence from strains of different DTUs. This new repeat was mostly on intergenic regions, and upstream and downstream regions of the 241 nt repeat were enriched in surface protein genes. RNAseq analysis revealed that the repeat was part of processed mRNAs and was predominantly found in the 3′ untranslated regions (UTRs) of genes of multigenic families encoding surface proteins. Moreover, we detected a correlation between the presence of the repeat in the 3′UTR of multigenic family genes and the level of differential expression of these genes when comparing epimastigote and trypomastigote transcriptomes. These data suggest that this sequence plays a role in the posttranscriptional regulation of the expression of multigenic families. | pt_BR |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | pt_BR |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | pt_BR |
dc.format.extent | 1235 | pt_BR |
dc.language | eng | pt_BR |
dc.publisher | MDPI | pt_BR |
dc.relation.ispartof | Genes | pt_BR |
dc.rights | Open Access | pt_BR |
dc.title | Identification of novel interspersed DNA repetitive elements in the Trypanosoma cruzi genome associated with the 3′UTRs of surface multigenic families | pt_BR |
dc.type | Article | pt_BR |
dc.identifier.doi | 10.3390/genes11101235 | - |
dc.identifier.url | https://doi.org/10.3390/genes11101235 | - |
dc.contributor.external | Universidade Federal de São Paulo (UNIFESP) | pt_BR |
dc.contributor.external | Centro Universitário São Camilo | pt_BR |
dc.publisher.city | Basel | pt_BR |
dc.identifier.citationvolume | 11 | - |
dc.identifier.citationissue | 10 | - |
dc.subject.keyword | Trypanosoma cruzi | pt_BR |
dc.subject.keyword | genome | pt_BR |
dc.subject.keyword | repeats | pt_BR |
dc.subject.keyword | 3′UTR | pt_BR |
dc.subject.keyword | multigenic family | pt_BR |
dc.relation.ispartofabbreviated | Genes | pt_BR |
dc.identifier.citationabnt | v. 11, n. 10, 1235, out. 2020 | - |
dc.identifier.citationvancouver | 2020 Oct;11(10):1235 | - |
dc.publisher.country | Switzerland | pt_BR |
dc.contributor.butantan | Calderano, Simone Guedes|:Pesquisador|:Laboratório de Parasitologia:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:PrimeiroAutor | pt_BR |
dc.contributor.butantan | Nishiyama Junior, Milton Yutaka|:Pesquisador|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):Laboratório Especial de Toxinologia Aplicada (LETA) | pt_BR |
dc.contributor.butantan | Nunes, Nathan de Oliveira|:Aluno|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):Laboratório Especial de Toxinologia Aplicada (LETA) | pt_BR |
dc.contributor.butantan | Reis, Marcelo da Silva|:Pesquisador|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):Laboratório Especial de Ciclo Celular | pt_BR |
dc.contributor.butantan | Patané, José Salvatore Leister|:Técnico|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):Laboratório Especial de Ciclo Celular | pt_BR |
dc.contributor.butantan | Da Cunha, Julia Pinheiro Chagas|:Pesquisador|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):Laboratório Especial de Ciclo Celular | pt_BR |
dc.contributor.butantan | Elias, Maria Carolina|:Pesquisador|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS):Laboratório Especial de Ciclo Celular|:Autor de correspondência | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/50050-2 | pt_BR |
dc.sponsorship.butantan | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦306199/2018-1 | pt_BR |
dc.identifier.bvscc | BR78.1 | - |
dc.identifier.bvsdb | IBProd | - |
item.openairetype | Article | - |
item.fulltext | Sem Texto completo | - |
item.grantfulltext | none | - |
crisitem.journal.journalissn | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.journal.journaleissn | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.dept | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.dept | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.dept | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.dept | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.dept | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.dept | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.dept | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.dept | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.dept | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.orcid | http://orcid.org/0000-0002-2410-0562 | - |
crisitem.author.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.orcid | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.parentorg | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.parentorg | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
crisitem.author.parentorg | #PLACEHOLDER_PARENT_METADATA_VALUE# | - |
Appears in Collections: | Artigos de periódicos |
Show simple item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.