Venom complexity of Bothrops atrox (common lancehead) siblings

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dc.contributorLaboratório de Herpetologiapt_BR
dc.contributor.authorHatakeyama, Daniela Mikipt_BR
dc.contributor.authorTasima, Lidia Jorgept_BR
dc.contributor.authorTobar, Cesar Adolfo Bravopt_BR
dc.contributor.authorSilva, Caroline Serinopt_BR
dc.contributor.authorTashima, Alexandre Keijipt_BR
dc.contributor.authorFabri, Carolinept_BR
dc.contributor.authorAguiar, Weslei da Silvapt_BR
dc.contributor.authorGalizio, Nathália da Costapt_BR
dc.contributor.authorLima, Eduardo Oliveira Venâncio dept_BR
dc.contributor.authorKavazoi, Victor Koitipt_BR
dc.contributor.authorGutiérrez-Marín, Juan Davidpt_BR
dc.contributor.authorFarias, Iasmim Baptista dept_BR
dc.contributor.authorSant'anna, Sávio Stefaninipt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorMorais-Zani, Karenpt_BR
dc.contributor.authorTanaka-Azevedo, Anita Mitikopt_BR
dc.identifier.citationHatakeyama DM, Tasima LJ, Tobar CAB, Silva CS, Tashima AK, Fabri C, et al. Venom complexity of Bothrops atrox (common lancehead) siblings. Journal of Venomous Animals and Toxins Including Tropical Diseases. 2020 Oct;26:20200018. doi:10.1590/1678-9199-jvatitd-2020-0018.pt_BR
dc.description.abstractBackground: Variability in snake venoms is a well-studied phenomenon. However, sex-based variation of Bothrops atrox snake venom using siblings is poorly investigated. Bothrops atrox is responsible for the majority of snakebite accidents in the Brazilian Amazon region. Differences in the venom composition of Bothrops genus have been linked to several factors such as ontogeny, geographical distribution, prey preferences and sex. Thus, in the current study, venom samples of Bothrops atrox male and female siblings were analyzed in order to compare their biochemical and biological characteristics. Methods: Venoms were collected from five females and four males born from a snake captured from the wild in São Bento (Maranhão, Brazil), and kept in the Laboratory of Herpetology of Butantan Intitute. The venoms were analyzed individually and as a pool of each gender. The assays consisted in protein quantification, 1-DE, mass spectrometry, proteolytic, phospholipase A2, L-amino acid oxidase activities, minimum coagulant dose upon plasma, minimum hemorrhagic dose and lethal dose 50%. Results: Electrophoretic profiles of male’s and female’s venom pools were quite similar, with minor sex-based variation. Male venom showed higher LAAO, PLA2 and hemorrhagic activities, while female venom showed higher coagulant activity. On the other hand, the proteolytic activities did not show statistical differences between pools, although some individual variations were observed. Meanwhile, proteomic profile revealed 112 different protein compounds; of which 105 were common proteins of female’s and male’s venom pools and seven were unique to females. Despite individual variations, lethality of both pools showed similar values. Conclusion: Although differences between female and male venoms were observed, our results show that individual variations are significant even between siblings, highlighting that biological activities of venoms and its composition are influenced by other factors beyond gender.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.publisherCenter for the Study of Venoms and Venomous Animals (CEVAP)pt_BR
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseasespt_BR
dc.rightsOpen Accesspt_BR
dc.titleVenom complexity of Bothrops atrox (common lancehead) siblingspt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)pt_BR
dc.subject.keywordBothrops atroxpt_BR
dc.subject.keywordSnake venompt_BR
dc.subject.keywordIndividual variationpt_BR
dc.relation.ispartofabbreviatedJournal of Venomous Animals and Toxins Including Tropical Diseasespt_BR
dc.identifier.citationabntv. 26, 20200018, out. 2020-
dc.identifier.citationvancouver2020 Oct;26:20200018-
dc.contributor.butantanHatakeyama, Daniela Miki|:Aluno|:Laboratório de Herpetologia|:PrimeiroAutorpt_BR
dc.contributor.butantanTasima, Lidia Jorge|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanTobar, Cesar Adolfo Bravo|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanSilva, Caroline Serino|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanFabri, Caroline|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanAguiar, Weslei da Silva|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanGalizio, Nathália da Costa|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanLima, Eduardo Oliveira Venâncio de|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanKavazoi, Victor Koiti|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanGutiérrez-Marín, Juan David|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanFarias, Iasmim Baptista de|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanMorais-Zani, Karen de|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Laboratório de Herpetologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16908-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/20106-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01890-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25786-0pt_BR
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