Biological effects of an oxyphytosterol generated by β-Sitosterol ozonization
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DC Field | Value | Language |
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dc.contributor | Laboratório de Desenvolvimento e Inovação | pt_BR |
dc.contributor.author | Takayasu, Bianca Sayuri | pt_BR |
dc.contributor.author | Martins, Igor R. | pt_BR |
dc.contributor.author | Garnique, Anali M.B. | pt_BR |
dc.contributor.author | Miyamoto, Sayuri | pt_BR |
dc.contributor.author | Machado-Santelli, Gláucia M. | pt_BR |
dc.contributor.author | Onuki, Janice | pt_BR |
dc.date.accessioned | 2020-11-04T20:26:06Z | - |
dc.date.available | 2020-11-04T20:26:06Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Takayasu BS, Martins IR., Garnique AM.B., Miyamoto S, Machado-Santelli GM., Onuki J. Biological effects of an oxyphytosterol generated by β-Sitosterol ozonization. Arch. Biochem. Biophys.. 2020 Oct;696:108654. doi:10.1016/j.abb.2020.108654. | pt_BR |
dc.identifier.issn | 0003-9861 | - |
dc.identifier.issn | 1096-0384 | - |
dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/3299 | - |
dc.description.abstract | β-Sitosterol (βSito) is the most abundant phytosterol found in vegetable oils, grains such as wheat, beans, and corn, and in many phytosterol-enriched foods. It is prone to oxidation by reactive oxygen species, such as ozone, leading to the formation of oxyphytosterols. A better understanding regarding the biological effects and mechanism of action of oxyphytosterols is required since the beneficial and adverse side effects of these compounds on human health remain highly controversial. In this work, we investigated the biological effects of β-Secosterol (βSec), a new oxyphytosterol generated by the reaction of βSito with ozone. Treatment of HepG2 cells with βSito or βSec (0.1–100 μM) for 24, 48, and 72 h induced a dose-dependent reduction of cell viability in the MTT assay, with βSec showing higher efficacy than βSito. However, βSec presented a lower potency than βSito, showing IC50 = 37.32 μM, higher than βSito (IC50 = 0.23 μM) at 48 h. Cell cycle analyses by flow cytometry showed a slight decrease of G1 phase with βSito 0.5 μM, but a significant cell cycle arrest at the G0/G1 phase in the treatment for 48 h with βSec 20 μM (62.69 ± 2.15%, p < 0.05) and βSec 40 μM (66.96 ± 5.39%, p < 0.0001) when compared to control (56.97 ± 2.60%). No suggestion of apoptosis was indicated by flow cytometry data. Also, βSec (20 and 40 μM) reduced the mitotic index. In the analysis with a confocal laser-scanning microscope, no alterations in cell morphology were observed with βSito (0.5 μM). Nevertheless, round-shaped cells, abnormal nuclear morphology with shrinkage, and formation of microtubules clusters were observed in the treatment with βSec, indicating a disruption in the microtubules network organization. N-acetyl-l-cysteine was not able to inhibit any of these cellular effects, indicating a lack of involvement of oxidative stress in the mechanism of action of βSec. Although not further investigated in this study, it was discussed the hypothesis that covalent adduct formation with lysine residues of proteins, could play an important role in the biological effects elicited by βSec. Elucidation of the primary cellular processes induced by βSec provides the essential knowledge to be aware of its potential adverse side effects or therapeutic use of this oxyphytosterol. | pt_BR |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | pt_BR |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | pt_BR |
dc.description.sponsorship | Financiadora de Estudos e Projetos (FINEP) | pt_BR |
dc.description.sponsorship | Fundação Butantan | pt_BR |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | pt_BR |
dc.format.extent | 108654 | pt_BR |
dc.language | eng | pt_BR |
dc.publisher | Elsevier | pt_BR |
dc.relation.ispartof | Archives of Biochemistry and Biophysics | pt_BR |
dc.title | Biological effects of an oxyphytosterol generated by β-Sitosterol ozonization | pt_BR |
dc.type | Article | pt_BR |
dc.identifier.doi | https://repositorio.butantan.gov.br/handle/butantan/3299 | - |
dc.identifier.doi | https://repositorio.butantan.gov.br/handle/butantan/3299 | - |
dc.identifier.doi | 10.1016/j.abb.2020.108654 | - |
dc.identifier.url | https://doi.org/10.1016/j.abb.2020.108654 | - |
dc.contributor.external | Universidade de São Paulo (USP) | pt_BR |
dc.contributor.external | Universidade Federal de São Paulo (UNIFESP) | pt_BR |
dc.publisher.city | San Diego | pt_BR |
dc.identifier.citationvolume | 696 | - |
dc.subject.keyword | Phytosterol | pt_BR |
dc.subject.keyword | Oxyphytosterols | pt_BR |
dc.subject.keyword | βSec | pt_BR |
dc.subject.keyword | Cytotoxicity | pt_BR |
dc.subject.keyword | Microtubules | pt_BR |
dc.subject.keyword | Cell cycle | pt_BR |
dc.subject.keyword | Ozone | pt_BR |
dc.relation.ispartofabbreviated | Arch. Biochem. Biophys. | pt_BR |
dc.identifier.citationabnt | v. 696, 108654, out. 2020 | - |
dc.identifier.citationvancouver | 2020 Oct;696:108654 | - |
dc.publisher.country | United States | pt_BR |
dc.contributor.butantan | Takayasu, Bianca Sayuri|:Aluno|:Laboratório de Desenvolvimento e Inovação|:PrimeiroAutor | pt_BR |
dc.contributor.butantan | Onuki, Janice|:Pesquisador|:Laboratório de Desenvolvimento e Inovação|:Autor de correspondência | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07937–8 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/17,177–6 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/14,801–8 | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2019/19,506–8 | pt_BR |
dc.sponsorship.butantan | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦303,464/2017–8 | pt_BR |
dc.sponsorship.butantan | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦484,628/2013–5 | pt_BR |
dc.sponsorship.butantan | Financiadora de Estudos e Projetos (FINEP)¦¦ | pt_BR |
dc.sponsorship.butantan | Fundação Butantan¦¦ | pt_BR |
dc.sponsorship.butantan | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦ | pt_BR |
dc.identifier.bvscc | BR78.1 | - |
dc.identifier.bvsdb | IBProd | - |
item.openairetype | Article | - |
item.fulltext | Sem Texto completo | - |
item.grantfulltext | none | - |
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