Genetic and virulence characteristics of a hybrid atypical enteropathogenic and uropathogenic Escherichia coli (aEPEC/UPEC) strain

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorValiatti, Tiago B.pt_BR
dc.contributor.authorSantos, Fernanda F.pt_BR
dc.contributor.authorSantos, Ana C. M.pt_BR
dc.contributor.authorNascimento, Júllia A. S.pt_BR
dc.contributor.authorSilva, Rosa M.pt_BR
dc.contributor.authorCarvalho, Eneaspt_BR
dc.contributor.authorSinigaglia, Ritapt_BR
dc.contributor.authorGomes, Tânia A. T.pt_BR
dc.date.accessioned2020-11-26T13:07:07Z-
dc.date.available2020-11-26T13:07:07Z-
dc.date.issued2020pt_BR
dc.identifier.citationValiatti TB., Santos FF., Santos AC.M., Nascimento JA.S., Silva RM., Carvalho E, et al. Genetic and virulence characteristics of a hybrid atypical enteropathogenic and uropathogenic Escherichia coli (aEPEC/UPEC) strain. Front. Cell. Infect. Microbiol.. 2020 Sept;10:492. doi:10.3389/fcimb.2020.00492.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3305-
dc.description.abstractHybrid strains of Escherichia coli combine virulence traits of diarrheagenic (DEC) and extraintestinal pathogenic E. coli (ExPEC), but it is poorly understood whether these combined features improve the virulence potential of such strains. We have previously identified a uropathogenic E. coli (UPEC) strain (UPEC 252) harboring the eae gene that encodes the adhesin intimin and is located in the locus of enterocyte effacement (LEE) pathogenicity island. The LEE-encoded proteins allow enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) to form attaching and effacing (A/E) lesions in enterocytes. We sought to characterize UPEC 252 through whole-genome sequencing and phenotypic virulence assays. Genome analysis unveiled that this strain harbors a complete LEE region, with more than 97% of identity comparing to E2348/69 (EPEC) and O157:H7 Sakai (EHEC) prototype strains, which was functional, since UPEC 252 expressed the LEE-encoded proteins EspB and intimin and induced actin accumulation foci in HeLa cells. Phylogenetic analysis performed comparing 1,000 single-copy shared genes clustered UPEC 252 with atypical EPEC strains that belong to the sequence type 10, phylogroup A. Additionally, UPEC 252 was resistant to the bactericidal power of human serum and colonized cells of the urinary (T24 and HEK293-T) and intestinal (Caco-2 and LS174T) tracts. Our findings suggest that UPEC 252 is an atypical EPEC strain that emerges as a hybrid strain (aEPEC/UPEC), which could colonize new niches and potentially cause intestinal and extraintestinal infections.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extent492pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Cellular and Infection Microbiologypt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleGenetic and virulence characteristics of a hybrid atypical enteropathogenic and uropathogenic Escherichia coli (aEPEC/UPEC) strainpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3389/fcimb.2020.00492pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fcimb.2020.00492pt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.identifier.citationvolume10pt_BR
dc.subject.keywordEscherichia colipt_BR
dc.subject.keywordUPECpt_BR
dc.subject.keywordhybrid pathogenic strainpt_BR
dc.subject.keywordvirulence factorpt_BR
dc.subject.keywordExPECpt_BR
dc.subject.keywordurinary tract infectionpt_BR
dc.subject.keywordatypical EPECpt_BR
dc.relation.ispartofabbreviatedFront Cell Infect Microbiolpt_BR
dc.identifier.citationabntv. 10, 492, set. 2020pt_BR
dc.identifier.citationvancouver2020 Sept;10:492pt_BR
dc.contributor.butantanCarvalho, Eneas|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/21947-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/17353-7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.languageiso639-1English-
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