Toposome: source of antimicrobial molecules in the gonads of the sea urchin Lytechinus variegatus (Lamarck, 1816)

Full metadata record
DC FieldValueLanguage
dc.contributorLaboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorFigueiredo, Débora Alvares Leitept_BR
dc.contributor.authorSantos, Wellington da Silvapt_BR
dc.contributor.authorMontoni, Fabiopt_BR
dc.contributor.authorIwai, Leo Keipt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.date.accessioned2020-12-17T13:28:02Z-
dc.date.available2020-12-17T13:28:02Z-
dc.date.issued2021pt_BR
dc.identifier.citationFigueiredo DAL, Santos WS, Montoni F, Iwai LK, Silva Junior PI. Toposome: source of antimicrobial molecules in the gonads of the sea urchin Lytechinus variegatus (Lamarck, 1816). Fish Shellfish Immunol.. 2020 Dec;in press. doi:10.1016/j.fsi.2020.11.023.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3379-
dc.description.abstractSea urchins live in a challenging environment that requires rapid and efficient responses against pathogens and invaders. This response may be also important in reproductive processes once males and females release their gametes into water. In addition, the gonads are organs with dual function: reproductive organ and nutrient reserve, therefore it needs efficient protective mechanisms to preserve the nutrients as well as the reproductive cells. The aim of this study was to evaluate the presence and characterize antimicrobial molecules in the male and female gonads of the sea urchin Lytechinus variegatus. Through HPLC purification, antimicrobial activity test and mass spectrometry several antimicrobial molecules were found in the gonads of both gender. Computational in silico analyses showed that they are fragments of a glycoprotein called toposome, also known as major yolk protein (MYP) which is one of the major proteins found in the gonads. Although different functions have been reported for this protein, this is the first description of a direct antimicrobial activity in Lytechinus variegatus. The results indicate that when undergoing proteolysis the toposome generates different fragments with antimicrobial activity which may indicate the importance of a rapid defense response strategy against invading microorganisms in the gonads used by both males and females sea urchins.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.languageengpt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFish & Shellfish Immunologypt_BR
dc.titleToposomept_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.fsi.2020.11.023pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.fsi.2020.11.023pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.title.subsource of antimicrobial molecules in the gonads of the sea urchin Lytechinus variegatus (Lamarck, 1816)pt_BR
dc.subject.keywordInnate immune systempt_BR
dc.subject.keywordMarine natural compoundspt_BR
dc.subject.keywordGender differencespt_BR
dc.subject.keywordEcological behaviorpt_BR
dc.subject.keywordAntimicrobial proteinpt_BR
dc.subject.keywordReproductionpt_BR
dc.relation.ispartofabbreviatedFish Shellfish Immunol.pt_BR
dc.identifier.citationabntv. 109, p. 51-61, fev. 2021pt_BR
dc.identifier.citationvancouver2021 Feb;109:51-61pt_BR
dc.contributor.butantanFigueiredo, Débora Alvares Leite|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:PrimeiroAutorpt_BR
dc.contributor.butantanSantos, Wellington da Silva|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanMontoni, Fabio|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanIwai, Leo Kei|:Pesquisador:Docente colaborador PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA)|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦001pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-CeTICS CEPIDpt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/04000–3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/17943–6pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦472744/2012–7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
item.openairetypeArticle-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0001-6619-6489-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

Existing users please Login
10.1016j.fsi.2020.11.023.pdf
Description:
Size: 6.56 MB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.