Toposome: source of antimicrobial molecules in the gonads of the sea urchin Lytechinus variegatus (Lamarck, 1816)

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dc.contributor(LETA) Lab. Toxinologia Aplicadapt_BR
dc.contributor(CeTICS) Centro de Toxinas, Resposta-imune e Sinalização Celularpt_BR
dc.contributor.authorFigueiredo, Débora Alvares Leitept_BR
dc.contributor.authorSantos, Wellington da Silvapt_BR
dc.contributor.authorMontoni, Fabiopt_BR
dc.contributor.authorIwai, Leo Keipt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.date.accessioned2020-12-17T13:28:02Z-
dc.date.available2020-12-17T13:28:02Z-
dc.date.issued2021pt_BR
dc.identifier.citationFigueiredo DAL, Santos WS, Montoni F, Iwai LK, Silva Junior PI. Toposome: source of antimicrobial molecules in the gonads of the sea urchin Lytechinus variegatus (Lamarck, 1816). Fish Shellfish Immunol.. 2021 Feb;109:51-61. doi:10.1016/j.fsi.2020.11.023.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3379-
dc.description.abstractSea urchins live in a challenging environment that requires rapid and efficient responses against pathogens and invaders. This response may be also important in reproductive processes once males and females release their gametes into water. In addition, the gonads are organs with dual function: reproductive organ and nutrient reserve, therefore it needs efficient protective mechanisms to preserve the nutrients as well as the reproductive cells. The aim of this study was to evaluate the presence and characterize antimicrobial molecules in the male and female gonads of the sea urchin Lytechinus variegatus. Through HPLC purification, antimicrobial activity test and mass spectrometry several antimicrobial molecules were found in the gonads of both gender. Computational in silico analyses showed that they are fragments of a glycoprotein called toposome, also known as major yolk protein (MYP) which is one of the major proteins found in the gonads. Although different functions have been reported for this protein, this is the first description of a direct antimicrobial activity in Lytechinus variegatus. The results indicate that when undergoing proteolysis the toposome generates different fragments with antimicrobial activity which may indicate the importance of a rapid defense response strategy against invading microorganisms in the gonads used by both males and females sea urchins.pt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent51-61pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFish & Shellfish Immunologypt_BR
dc.rightsRestricted accesspt_BR
dc.titleToposome: source of antimicrobial molecules in the gonads of the sea urchin Lytechinus variegatus (Lamarck, 1816)pt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.fsi.2020.11.023pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.fsi.2020.11.023pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume109pt_BR
dc.subject.keywordInnate immune systempt_BR
dc.subject.keywordMarine natural compoundspt_BR
dc.subject.keywordGender differencespt_BR
dc.subject.keywordEcological behaviorpt_BR
dc.subject.keywordAntimicrobial proteinpt_BR
dc.subject.keywordreproductionpt_BR
dc.relation.ispartofabbreviatedFish Shellfish Immunolpt_BR
dc.identifier.citationabntv. 109, p. 51-61, fev. 2021pt_BR
dc.identifier.citationvancouver2021 Feb;109:51-61pt_BR
dc.contributor.butantanFigueiredo, Débora Alvares Leite|:Aluno|:(LETA) Lab. Toxinologia Aplicada:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:PrimeiroAutorpt_BR
dc.contributor.butantanSantos, Wellington da Silva|:Aluno|:(LETA) Lab. Toxinologia Aplicada:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanMontoni, Fabio|:Aluno|:(LETA) Lab. Toxinologia Aplicada:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanIwai, Leo Kei|:Pesquisador:Docente colaborador PPGTOX|:(LETA) Lab. Toxinologia Aplicada:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:(LETA) Lab. Toxinologia Aplicada|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2013/07467-CeTICS CEPIDpt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/04000–3pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/17943–6pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦472744/2012–7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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item.languageiso639-1English-
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crisitem.author.orcid0000-0002-1571-7763-
crisitem.author.orcid0000-0001-6619-6489-
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