P-MAPA, a fungi-derived Immunomodulatory compound, Induces a proinflammatory response in a human whole blood model

Full metadata record
DC FieldValueLanguage
dc.contributorLab. Imunoquímicapt_BR
dc.contributor.authorGonçalves, Mariana Torrentept_BR
dc.contributor.authorSquaiella-Baptistão, Carla Cristinapt_BR
dc.contributor.authorPidde-Queiroz, Gisellept_BR
dc.contributor.authorLopes, Priscila Hesspt_BR
dc.contributor.authorNunes, Iseu da Silvapt_BR
dc.contributor.authorTambourgi, Denise Vilarinhopt_BR
dc.date.accessioned2020-12-17T18:21:52Z-
dc.date.available2020-12-17T18:21:52Z-
dc.date.issued2020pt_BR
dc.identifier.citationGonçalves MT, Squaiella-Baptistao CC., Pidde-Queiroz G, Lopes PH, Nunes IS, Tambourgi DV. P-MAPA, a fungi-derived Immunomodulatory compound, Induces a proinflammatory response in a human whole blood model. Mediators Inflamm.. 2020 Nov;2020:83138. doi:10.1155/2020/8831389.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3388-
dc.description.abstractP-MAPA is a complex compound, derived from Aspergillus oryzae cultures, that has shown immunomodulatory properties in infection and cancer animal models. Despite promising results in these models, the mechanisms of cellular activation by P-MAPA, suggested to be Toll-like receptor- (TLR-) dependent, and its effect on human immune cells, remain unclear. Using an ex vivo model of human whole blood, the effects of P-MAPA on complement system activation, production of cytokines, and the expression of complement receptors (CD11b, C5aR, and C3aR), TLR2, TLR4, and the coreceptor CD14 were analyzed in neutrophils and monocytes. P-MAPA induced complement activation in human blood, detected by increased levels of C3a, C5a, and SC5b-9 in plasma. As a consequence, CD11b expression increased and C5aR decreased upon activation, while C3aR expression remained unchanged in leukocytes. TLR2 and TLR4 expressions were not modulated by P-MAPA treatment on neutrophils, but TLR4 expression was reduced in monocytes, while CD14 expression increased in both cell types. P-MAPA also induced the production of TNF-α, IL-8, and IL-12 and oxidative burst, measured by peroxynitrite levels, in human leukocytes. Complement inhibition with compstatin showed that P-MAPA-induced complement activation drives modulation of C5aR, but not of CD11b, suggesting that P-MAPA acts through both complement-dependent and complement-independent mechanisms. Compstatin also significantly reduced the peroxynitrite generation. Altogether, our results show that P-MAPA induced proinflammatory response in human leukocytes, which is partially mediated by complement activation. Our data contribute to elucidate the complement-dependent and complement-independent mechanisms of P-MAPA, which ultimately result in immune cell activation and in its immunomodulatory properties in infection and cancer animal modelspt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent8831389pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofMediators of Inflammationpt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleP-MAPA, a fungi-derived Immunomodulatory compound, Induces a proinflammatory response in a human whole blood modelpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1155/2020/8831389pt_BR
dc.identifier.urlhttps://doi.org/10.1155/2020/8831389pt_BR
dc.contributor.externalFarmabrasilispt_BR
dc.identifier.citationvolume2020pt_BR
dc.relation.ispartofabbreviatedMediators Inflammpt_BR
dc.identifier.citationabntv. 2020, 83138, nov. 2020pt_BR
dc.identifier.citationvancouver2020 Nov;2020:83138pt_BR
dc.contributor.butantanGonçalves, Mariana Torrente|:Aluno|:Lab. Imunoquímica|:PrimeiroAutorpt_BR
dc.contributor.butantanSquaiella-Baptistão, Carla Cristina|:Pesquisador|:Lab. Imunoquímicapt_BR
dc.contributor.butantanPidde-Queiroz, Giselle|:Pesquisador|:Lab. Imunoquímicapt_BR
dc.contributor.butantanLopes, Priscila Hess|:Aluno|:Lab. Imunoquímicapt_BR
dc.contributor.butantanTambourgi, Denise Vilarinho|:Pesquisador:Docente permanente PPGTOX|:Lab. Imunoquímica|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2012/05306-8pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1English-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-4187-798X-
crisitem.author.orcid0000-0002-7622-5981-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-1896-9074-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

10.1155.2020.8831389.pdf
Description:
Size: 1.2 MB
Format: Adobe PDF
View/Open
Show simple item record

This item is licensed under a Creative Commons License Creative Commons