Size matters: an evaluation of the molecular basis of ontogenetic modifications in the composition of Bothrops jararacussu snake venom

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dc.contributorLab. Imunopatologiapt_BR
dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributorLaboratório de Coleções Zoológicaspt_BR
dc.contributor.authorFreitas-de-Sousa, Luciana Aparecidapt_BR
dc.contributor.authorNachtigall, Pedro Gabrielpt_BR
dc.contributor.authorPortes Junior, José Antoniopt_BR
dc.contributor.authorHolding, Matthew L.pt_BR
dc.contributor.authorNystrom, Gunnar S.pt_BR
dc.contributor.authorEllsworth, Schyler A.pt_BR
dc.contributor.authorGuimarães, Noranathan da Costapt_BR
dc.contributor.authorTioyama, Emilly Campospt_BR
dc.contributor.authorOrtiz, Flora Roncolattopt_BR
dc.contributor.authorSilva, Bruno Rocha dapt_BR
dc.contributor.authorKunz, Tobias S.pt_BR
dc.contributor.authorJunqueira-de-Azevedo, Inácio de Loiola Meirellespt_BR
dc.contributor.authorGrazziotin, Felipe Gobbipt_BR
dc.contributor.authorRokyta, Darin R.pt_BR
dc.contributor.authorMoura-da-Silva, Ana Mariapt_BR
dc.date.accessioned2020-12-18T18:13:29Z-
dc.date.available2020-12-18T18:13:29Z-
dc.date.issued2020pt_BR
dc.identifier.citationSousa LAF, Nachtigall PG, Portes Junior JA, Holding ML., Nystrom GS., Ellsworth SA., et al. Size matters: an evaluation of the molecular basis of ontogenetic modifications in the composition of Bothrops jararacussu snake venom. Toxins. 2020 Dec;12(12):791. doi:10.3390/toxins12120791.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3414-
dc.description.abstractOntogenetic changes in venom composition have been described in Bothrops snakes, but only a few studies have attempted to identify the targeted paralogues or the molecular mechanisms involved in modifications of gene expression during ontogeny. In this study, we decoded B. jararacussu venom gland transcripts from six specimens of varying sizes and analyzed the variability in the composition of independent venom proteomes from 19 individuals. We identified 125 distinct putative toxin transcripts, and of these, 73 were detected in venom proteomes and only 10 were involved in the ontogenetic changes. Ontogenetic variability was linearly related to snake size and did not correspond to the maturation of the reproductive stage. Changes in the transcriptome were highly predictive of changes in the venom proteome. The basic myotoxic phospholipases A2 (PLA2s) were the most abundant components in larger snakes, while in venoms from smaller snakes, PIII-class SVMPs were the major components. The snake venom metalloproteinases (SVMPs) identified corresponded to novel sequences and conferred higher pro-coagulant and hemorrhagic functions to the venom of small snakes. The mechanisms modulating venom variability are predominantly related to transcriptional events and may consist of an advantage of higher hematotoxicity and more efficient predatory function in the venom from small snakes.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM)pt_BR
dc.format.extent791pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleSize matters: an evaluation of the molecular basis of ontogenetic modifications in the composition of Bothrops jararacussu snake venompt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/toxins12120791pt_BR
dc.identifier.urlhttps://doi.org/10.3390/toxins12120791pt_BR
dc.contributor.externalFlorida State Universitypt_BR
dc.contributor.externalFundação de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD)pt_BR
dc.identifier.citationvolume12pt_BR
dc.identifier.citationissue12pt_BR
dc.subject.keywordBothrops jararacussupt_BR
dc.subject.keywordontogenetic variabilitypt_BR
dc.subject.keywordtranscriptomept_BR
dc.subject.keywordproteomept_BR
dc.subject.keywordmetalloproteinasespt_BR
dc.subject.keywordphospholipasespt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 12, n. 12, 791, dez. 2020pt_BR
dc.identifier.citationvancouver2020 Dec;12(12):791pt_BR
dc.contributor.butantanFreitas-de-Sousa, Luciana Aparecida|:Aluno:Docente colaborador|:Lab. Imunopatologia|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanNachtigall, Pedro Gabriel|:Aluno|:Laboratório de Toxinologia Aplicadapt_BR
dc.contributor.butantanPortes Junior, José Antonio|:Aluno|:Laboratório de Coleções Zoológicaspt_BR
dc.contributor.butantanGuimarães, Noranathan da Costa|:Aluno|:Lab. Imunopatologiapt_BR
dc.contributor.butantanTioyama, Emilly Campos|:Aluno|:Lab. Imunopatologiapt_BR
dc.contributor.butantanOrtiz, Flora Roncolatto|:Aluno|:Laboratório de Coleções Zoológicaspt_BR
dc.contributor.butantanSilva, Bruno Rocha da|:Aluno|:Laboratório de Coleções Zoológicaspt_BR
dc.contributor.butantanKunz, Tobias S.|:Aluno|:Laboratório de Coleções Zoológicaspt_BR
dc.contributor.butantanJunqueira-de-Azevedo, Inácio de Loiola Meirelles|:Pesquisador:Docente permanente PPGTOX|:Laboratório de Toxinologia Aplicadapt_BR
dc.contributor.butantanGrazziotin, Felipe Gobbi|:Pesquisador|:Laboratório de Coleções Zoológicaspt_BR
dc.contributor.butantanMoura-da-Silva, Ana Maria|:Pesquisador:Docente permanente PPGTOX|:Lab. Imunopatologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/50127-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/24546-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/08589-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/26520-4pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25749-8pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/15753-8pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦303958/2018-9pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦304532/2013-4pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM)pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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