Mygalin: an acylpolyamine with bactericidal activity

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dc.contributorLab. Bacteriologiapt_BR
dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorEspinoza-Culupú, Abrahampt_BR
dc.contributor.authorMendes, Elizabethpt_BR
dc.contributor.authorVitorino, Hector Aguilarpt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.contributor.authorBorges, Monamaris Marquespt_BR
dc.identifier.citationEspinoza-Culupú AO, Mendes E, Vitorino HA, Silva Junior PI, Borges MM. Mygalin: an acylpolyamine with bactericidal activity. Front. Microbiol.. 2020 Jan;10:2928. doi:10.3389/fmicb.2019.02928.pt_BR
dc.description.abstractInappropriate use of antibiotics favors the selection and spread of resistant bacteria. To reduce the spread of these bacteria, finding new molecules with activity is urgent and necessary. Several polyamine analogs have been constructed and used to control microorganisms and tumor cells. Mygalin is a synthetic acylpolyamine, which are analogs of spermidine, derived from the hemolymph of the spider Acanthoscurria gomesiana. The effective activity of polyamines and their analogs has been associated with their structure. The presence of two acyl groups in the Mygalin structure may give this molecule a specific antibacterial activity. The aim of this study was to identify the mechanisms involved in the interaction of Mygalin with Escherichia coli to clarify its antimicrobial action. The results indicated that Mygalin exhibits intense dose and time-dependent bactericidal activity. Treatment of E. coli with this molecule caused membrane rupture, inhibition of DNA synthesis, DNA damage, and morphological changes. The esterase activity increased along with the intracellular production of reactive oxygen species (ROS) after treatment of the bacteria with Mygalin. In addition, this molecule was able to sequester iron and bind to LPS. We have shown that Mygalin has bactericidal activity with underlying mechanisms involving ROS generation and chelation of iron ions that are necessary for bacterial metabolism, which may contribute to its microbicidal activity. Taken together, our data suggest that Mygalin can be explored as a new alternative drug with antimicrobial potential against Gram-negative bacteria or other infectious agents.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCONCYTEC-FONDECYT Institution of Peruvian Statept_BR
dc.description.sponsorshipFundação Butantanpt_BR
dc.relation.ispartofFrontiers in Microbiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleMygalin: an acylpolyamine with bactericidal activitypt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalUniversity of Maryland, Baltimorept_BR
dc.subject.keywordoxidative stresspt_BR
dc.subject.keywordE. colipt_BR
dc.relation.ispartofabbreviatedFront Microbiolpt_BR
dc.identifier.citationabntv. 10, 2928, jan. 2020pt_BR
dc.identifier.citationvancouver2020 Jan;10:2928pt_BR
dc.contributor.butantanEspinoza-Culupú, Abraham|:Aluno|:Lab. Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanMendes, Elizabeth|:Colaborador|:Lab. Bacteriologiapt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributor.butantanBorges, Monamaris Marques|:Pesquisador|:Lab. Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/11212-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/04307-6pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦472744/2012-7pt_BR
dc.sponsorship.butantanCONCYTEC-FONDECYT Institution of Peruvian State¦¦N092-2016pt_BR
dc.sponsorship.butantanFundação Butantan¦¦pt_BR
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