Nucleosome landscape reflects phenotypic differences in Trypanosoma cruzi life forms

Full metadata record
DC FieldValueLanguage
dc.contributorLaboratório Especial de Ciclo Celularpt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorLima, Alex Ranieri Jerônimopt_BR
dc.contributor.authorde Araujo, Christiane Bezerrapt_BR
dc.contributor.authorPoubel, Saloê Bispopt_BR
dc.contributor.authorPatané, José Salvatore Leisterpt_BR
dc.contributor.authorSilber, Ariel M.pt_BR
dc.contributor.authorElias, Maria Carolinapt_BR
dc.contributor.authorDa Cunha, Julia Pinheiro Chagaspt_BR
dc.date.accessioned2021-01-28T17:37:01Z-
dc.date.available2021-01-28T17:37:01Z-
dc.date.issued2021-
dc.identifier.citationLima ARJ, de Araujo CB, Poubel SB, Patané JSL, Silber AM., Elias MC, et al. Nucleosome landscape reflects phenotypic differences in Trypanosoma cruzi life formsNucleosome landscape reflects phenotypic differences in Trypanosoma cruzi life forms. PLoS Pathog.. 2021 Jan;17(1):e1009272. doi:10.1371/journal.ppat.1009272.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3498-
dc.description.abstractTrypanosoma cruzi alternates between replicative and nonreplicative life forms, accompanied by a shift in global transcription levels and by changes in the nuclear architecture, the chromatin proteome and histone posttranslational modifications. To gain further insights into the epigenetic regulation that accompanies life form changes, we performed genome-wide high-resolution nucleosome mapping using two T. cruzi life forms (epimastigotes and cellular trypomastigotes). By combining a powerful pipeline that allowed us to faithfully compare nucleosome positioning and occupancy, more than 125 thousand nucleosomes were mapped, and approximately 20% of them differed between replicative and nonreplicative forms. The nonreplicative forms have less dynamic nucleosomes, possibly reflecting their lower global transcription levels and DNA replication arrest. However, dynamic nucleosomes are enriched at nonreplicative regulatory transcription initiation regions and at multigenic family members, which are associated with infective-stage and virulence factors. Strikingly, dynamic nucleosome regions are associated with GO terms related to nuclear division, translation, gene regulation and metabolism and, notably, associated with transcripts with different expression levels among life forms. Finally, the nucleosome landscape reflects the steady-state transcription expression: more abundant genes have a more deeply nucleosome-depleted region at putative 5’ splice sites, likely associated with trans-splicing efficiency. Taken together, our results indicate that chromatin architecture, defined primarily by nucleosome positioning and occupancy, reflects the phenotypic differences found among T. cruzi life forms despite the lack of a canonical transcriptional control context.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipInstituto Serrapilheirapt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extente1009272pt_BR
dc.languageengpt_BR
dc.relation.ispartofPLoS Pathogenspt_BR
dc.rightsOpen Accesspt_BR
dc.titleNucleosome landscape reflects phenotypic differences in Trypanosoma cruzi life formspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1371/journal.ppat.1009272pt_BR
dc.identifier.urlhttps://doi.org/10.1371/journal.ppat.1009272pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.identifier.citationvolume17pt_BR
dc.identifier.citationissue1pt_BR
dc.relation.ispartofabbreviatedPLoS Pathog.pt_BR
dc.identifier.citationabntv. 17, n. 1, e1009272, jan. 2021pt_BR
dc.identifier.citationvancouver2021 Jan;17(1):e1009272pt_BR
dc.contributor.butantanLima, Alex Ranieri Jerônimo|:Aluno|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:PrimeiroAutorpt_BR
dc.contributor.butantande Araujo, Christiane Bezerra|:Aluno|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanPoubel, Saloê Bispo|:Aluno|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanPatané, José Salvatore Leister|:Pesquisador|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanElias, Maria Carolina|:Pesquisador|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.contributor.butantanDa Cunha, Julia Pinheiro Chagas|:Pesquisador|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦18/15553-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦13/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦19/19690-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦18/14432-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦16/50050-2pt_BR
dc.sponsorship.butantanInstituto Serrapilheira¦¦1709-16865pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.grantfulltextembargo_29990101-
item.openairetypeArticle-
item.fulltextCom Texto completo-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0003-0466-8879-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

Existing users please Login
10.1371journal.ppat.1009272.pdf
Description:
Size: 4.54 MB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.