The potential anti-inflammatory and anti-nociceptive effects of rat hemopressin (PVNFKFLSH) in experimental arthritis

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dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorCamargo, Livia L.pt_BR
dc.contributor.authorDenadai-Souza, Alexandrept_BR
dc.contributor.authorYshii, Lidia M.pt_BR
dc.contributor.authorLima, Carlapt_BR
dc.contributor.authorTeixeira, Simone A.pt_BR
dc.contributor.authorCerqueira, Anderson R.A.pt_BR
dc.contributor.authorGewehr, Mayara C.F.pt_BR
dc.contributor.authorFernandes, Elizabeth S.pt_BR
dc.contributor.authorSchenka, André A.pt_BR
dc.contributor.authorMuscará, Marcelo N.pt_BR
dc.contributor.authorFerro, Emer S.pt_BR
dc.contributor.authorCosta, Soraia K.P.pt_BR
dc.identifier.citationCamargo LL., Denadai-Souza A, Yshii LM., Lima C, Teixeira SA., Cerqueira AR.A., et al. The potential anti-inflammatory and anti-nociceptive effects of rat hemopressin (PVNFKFLSH) in experimental arthritis. Eur. J. Pharmacol.. 2021 Jan;890:173636. doi:10.1016/j.ejphar.2020.173636.pt_BR
dc.description.abstractInflammatory arthritis, such as rheumatoid arthritis (RA), stands out as one of the main sources of pain and impairment to the quality of life. The use of hemopressin (PVNFKFLSH; Hp), an inverse agonist of type 1 cannabinoid receptor, has proven to be effective in producing analgesia in pain models, but its effect on neuro-inflammatory aspects of RA is limited. In this study, antigen-induced arthritis (AIA) was evoked by the intraarticular ( injection of methylated bovine serum albumin (mBSA) in male Sprague Dawley rats. Phosphate buffered saline (PBS)-injected ipsilateral knee joints or AIA contralateral were used as control. Nociceptive and inflammatory parameters such as knee joint oedema and leukocyte influx and histopathological changes were carried out in addition to the local measurement of interleukins (IL) IL-6, IL-1β, tumor necrosis factor-α and the immunoreactivity of the neuropeptides substance P (SP) and calcitonin gene related peptide (CGRP) in the spinal cord (lumbar L3-5 segments) of AIA rats. For 4 days, AIA rats were treated daily with a single administration of saline, Hp injected (10 or 20 μg/day,, Hp given orally (20 μg/Kg, p.o.) or indomethacin (Indo; 5 mg/Kg, i.p.). In comparison to the PBS control group, the induction of AIA produced a significant and progressive mono-arthritis condition. The degree of AIA severity progressively compromised the normal walking pattern and impaired mobility over the next four days in relation to PBS-injected rats or contralateral knee joints. In AIA rats, the reduction of the distance between footprints and disturbances of gait evidenced signs of nociception. This response worsened at day 4, and a loss of footprint from the ipsilateral hind paw was evident. Daily treatment of the animals with Hp either (10 and 20 μg/knee) or p.o. (20 μg/Kg) as well as Indo (5 mg/Kg, i.p.) ameliorated the impaired mobility in a time-dependent manner (P < 0.05). In parallel, the AIA-injected ipsilateral knee joints reach a peak of swelling 24 h after AIA induction, which persisted over the next four days in relation to PBS-injected rats or contralateral knee joints. There was a significant but not dose-dependent inhibitory effect produced by all dosages and routes of Hp treatments on AIA-induced knee joint swelling (P < 0.05). In addition, the increased synovial levels of MPO activity, total leukocytes number and IL-6, but not IL-1β, were significantly reduced by the lower dose of Hp. In conclusion, these results successfully demonstrate that Hp may represent a novel therapeutic strategy to treat RA, an effect which is unrelated to the proinflammatory actions of the neuropeptides CGRP and SPpt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipWeizmann Institute of Sciencept_BR
dc.relation.ispartofEuropean Journal of Pharmacologypt_BR
dc.rightsRestricted accesspt_BR
dc.titleThe potential anti-inflammatory and anti-nociceptive effects of rat hemopressin (PVNFKFLSH) in experimental arthritispt_BR
dc.contributor.externalUniversity of Glasgowpt_BR
dc.contributor.externalUniversity of Toulousept_BR
dc.contributor.externalTranslational Research Center for Gastrointestinal Disorders (TARGID)pt_BR
dc.contributor.externalVIB-KU Leuven Center for Brain & Disease Researchpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalInstituto de Pesquisa Pelé Pequeno Príncipept_BR
dc.contributor.externalFaculdades Pequeno Príncipe (FPP)pt_BR
dc.contributor.externalUniversidade Estadual de Campinas (UNICAMP)pt_BR
dc.contributor.externalWeizmann Institute of Sciencept_BR
dc.subject.keywordAntigen-induced arthritispt_BR
dc.relation.ispartofabbreviatedEur. J. Pharmacol.pt_BR
dc.identifier.citationabntv. 890, 173636, jan. 2021pt_BR
dc.identifier.citationvancouver2021 Jan;890:173636pt_BR
dc.contributor.butantanLima, Carla|:Pesquisador:Docente permanente PPGTOX|:Laboratório de Toxinologia Aplicadapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦302809/2016-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/04000-3pt_BR
dc.sponsorship.butantanWeizmann Institute of Science¦¦pt_BR
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