The ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events

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dc.contributorLCC - Laboratório de Ciclo Celularpt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorBagatelli, Felipe F Mpt_BR
dc.contributor.authorVitorino, Francisca Nathália de Lunapt_BR
dc.contributor.authorDa Cunha, Julia Pinheiro Chagaspt_BR
dc.contributor.authorOliveira, Carla Cpt_BR
dc.date.accessioned2021-06-24T20:19:59Z-
dc.date.available2021-06-24T20:19:59Z-
dc.date.issued2021pt_BR
dc.identifier.citationBagatelli FFM, Vitorino FNL, Da Cunha JPC, Oliveira CC. The ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events. Nucleic Acids Res.. 2021 June;49(12):7053–7074. doi:10.1093/nar/gkab494.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3861-
dc.description.abstractEukaryotic ribosome biogenesis is an elaborate process during which ribosomal proteins assemble with the pre-rRNA while it is being processed and folded. Hundreds of assembly factors (AF) are required and transiently recruited to assist the sequential remodeling events. One of the most intricate ones is the stepwise removal of the internal transcribed spacer 2 (ITS2), between the 5.8S and 25S rRNAs, that constitutes together with five AFs the pre-60S ‘foot’. In the transition from nucleolus to nucleoplasm, Nop53 replaces Erb1 at the basis of the foot and recruits the RNA exosome for the ITS2 cleavage and foot disassembly. Here we comprehensively analyze the impact of Nop53 recruitment on the pre-60S compositional changes. We show that depletion of Nop53, different from nop53 mutants lacking the exosome-interacting motif, not only causes retention of the unprocessed foot in late pre-60S intermediates but also affects the transition from nucleolar state E particle to subsequent nuclear stages. Additionally, we reveal that Nop53 depletion causes the impairment of late maturation events such as Yvh1 recruitment. In light of recently described pre-60S cryo-EM structures, our results provide biochemical evidence for the structural role of Nop53 rearranging and stabilizing the foot interface to assist the Nog2 particle formationpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent7053–7074pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofNucleic Acids Researchpt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/pt_BR
dc.titleThe ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation eventspt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BY-NCpt_BR
dc.identifier.doi10.1093/nar/gkab494pt_BR
dc.identifier.urlhttps://doi.org/10.1093/nar/gkab494pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.identifier.citationvolume49pt_BR
dc.identifier.citationissue12pt_BR
dc.relation.ispartofabbreviatedNucleic Acids Res.pt_BR
dc.identifier.citationabntv. 49, n. 12, p. 7053–7074, jun. 2021pt_BR
dc.identifier.citationvancouver2021 June;49(12):7053–7074pt_BR
dc.contributor.butantanVitorino, Francisca Nathália de Luna|:Aluno|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanDa Cunha, Julia Pinheiro Chagas|:Pesquisador|:Laboratório Especial de Ciclo Celular:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦15/06477-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦13/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/05776-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017-18344-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦15/06477-9pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
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