A metalloproteinase induces an inflammatory response in preadipocytes with the activation of COX signalling pathways and participation of endogenous phospholipases A2

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dc.contributorLab. Farmacologiapt_BR
dc.contributorCENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributorLab. Desenvolvimento e Inovação Industrialpt_BR
dc.contributor.authorMotta, Priscila Signorpt_BR
dc.contributor.authorLeiguez, Elbiopt_BR
dc.contributor.authorPortas, Viviane Barbosapt_BR
dc.contributor.authorTeixeira, Catarina de Fátima Pereirapt_BR
dc.date.accessioned2021-07-02T18:34:20Z-
dc.date.available2021-07-02T18:34:20Z-
dc.date.issued2021pt_BR
dc.identifier.citationJanovits PM, Leiguez E, Portas V, Teixeira CFP. A metalloproteinase induces an inflammatory response in preadipocytes with the activation of COX signalling pathways and participation of endogenous phospholipases A2. Biomolecules. 2021 June;11(7):921;. doi:10.3390/biom11070921.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3876-
dc.description.abstractMatrix metalloproteinases (MMPs) are proteolytic enzymes that have been associated with the pathogenesis of inflammatory diseases and obesity. Adipose tissue in turn is an active endocrine organ capable of secreting a range of proinflammatory mediators with autocrine and paracrine properties, which contribute to the inflammation of adipose tissue and adjacent tissues. However, the potential inflammatory effects of MMPs in adipose tissue cells are still unknown. This study investigates the effects of BmooMPα-I, a single-domain snake venom metalloproteinase (SVMP), in activating an inflammatory response by 3T3-L1 preadipocytes in culture, focusing on prostaglandins (PGs), cytokines, and adipocytokines biosynthesis and mechanisms involved in prostaglandin E2 (PGE2) release. The results show that BmooMPα-I induced the release of PGE2, prostaglandin I2 (PGI2), monocyte chemoattractant protein-1 (MCP-1), and adiponectin by preadipocytes. BmooMPα-I-induced PGE2 biosynthesis was dependent on group-IIA-secreted phospholipase A2 (sPLA2-IIA), cytosolic phospholipase A2-α (cPLA2-α), and cyclooxygenase (COX)-1 and -2 pathways. Moreover, BmooMPα-I upregulated COX-2 protein expression but not microsomal prostaglandin E synthase-1 (mPGES-1) expression. In addition, we demonstrate that the enzymatic activity of BmooMPα-I is essential for the activation of prostanoid synthesis pathways in preadipocytes. These data highlight preadipocytes as important targets for metalloproteinases and provide new insights into the contribution of these enzymes to the inflammation of adipose tissue and tissues adjacent to it.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipGlaxoSmithKline (GSK)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação Butantanpt_BR
dc.format.extent921pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofBiomoleculespt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleA metalloproteinase induces an inflammatory response in preadipocytes with the activation of COX signalling pathways and participation of endogenous phospholipases A2pt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/biom11070921pt_BR
dc.identifier.urlhttps://doi.org/10.3390/biom11070921pt_BR
dc.identifier.citationvolume11pt_BR
dc.identifier.citationissue7pt_BR
dc.subject.keywordmetalloproteinasept_BR
dc.subject.keywordpreadipocytespt_BR
dc.subject.keywordprostaglandinspt_BR
dc.subject.keywordadipokinespt_BR
dc.subject.keywordcytokinespt_BR
dc.relation.ispartofabbreviatedBiomoleculespt_BR
dc.identifier.citationabntv. 11, n. 7, 921, jun. 2021pt_BR
dc.identifier.citationvancouver2021 June;11(7):921;pt_BR
dc.contributor.butantanJanovits, Priscila Motta|:Aluno Egresso|:Lab. Farmacologia:Programa de Pós-Graduação em Ciências – Toxinologia (PPGTox)|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanLeiguez, Elbio|:Pós-Doc|:Lab. Farmacologia:CENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributor.butantanPortas, Viviane|:Outros|:CENTD - Centro de Excelência para Descoberta de Alvos Moleculares:Lab. Desenvolvimento e Inovação Industrialpt_BR
dc.contributor.butantanTeixeira, Catarina de Fátima Pereira|:Pesquisador|:Lab. Farmacologia:CENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/50040-4pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/197339pt_BR
dc.sponsorship.butantanGlaxoSmithKline (GSK)¦¦pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦310930/2019-7pt_BR
dc.sponsorship.butantanFundação Butantan¦¦2019–2021pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
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