Heat-Labile toxin from enterotoxigenic Escherichia coli causes systemic impairment in Zebrafish model

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dc.contributorLab. Bacteriologiapt_BR
dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributorLab. Fisiopatologiapt_BR
dc.contributor.authorHenrique, Camilapt_BR
dc.contributor.authorFalcão, Maria Alice Pimentelpt_BR
dc.contributor.authorPimenta, Luciana de Araujopt_BR
dc.contributor.authorMaleski, Adolfo Luis Almeidapt_BR
dc.contributor.authorLima, Carlapt_BR
dc.contributor.authorMitsunari, Thaíspt_BR
dc.contributor.authorSampaio, Sandra Coccuzzopt_BR
dc.contributor.authorLopes-Ferreira, Monicapt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.date.accessioned2021-07-08T20:03:49Z-
dc.date.available2021-07-08T20:03:49Z-
dc.date.issued2021pt_BR
dc.identifier.citationHenrique C, Falcão MAP, Pimenta LA, Maleski ALA, Lima C, Mitsunari T, et al. Heat-Labile toxin from enterotoxigenic Escherichia coli causes systemic impairment in Zebrafish model. Toxins. 2021 June;13(6):419. doi:10.3390/toxins13060419.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3885-
dc.description.abstractHeat-labile toxin I (LT-I), produced by strains of enterotoxigenic Escherichia coli (ETEC), causes profuse watery diarrhea in humans. Different in vitro and in vivo models have already elucidated the mechanism of action of this toxin; however, their use does not always allow for more specific studies on how the LT-I toxin acts in systemic tracts and intestinal cell lines. In the present work, zebrafish (Danio rerio) and human intestinal cells (Caco-2) were used as models to study the toxin LT-I. Caco-2 cells were used, in the 62nd passage, at different cell concentrations. LT-I was conjugated to FITC to visualize its transport in cells, as well as microinjected into the caudal vein of zebrafish larvae, in order to investigate its effects on survival, systemic traffic, and morphological formation. The internalization of LT-I was visualized in 3 × 104 Caco-2 cells, being associated with the cell membrane and nucleus. The systemic traffic of LT-I in zebrafish larvae showed its presence in the cardiac cavity, yolk, and regions of the intestine, as demonstrated by cardiac edema (100%), the absence of a swimming bladder (100%), and yolk edema (80%), in addition to growth limitation in the larvae, compared to the control group. There was a reduction in heart rate during the assessment of larval survival kinetics, demonstrating the cardiotoxic effect of LT-I. Thus, in this study, we provide essential new depictions of the features of LT-I.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent419pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleHeat-Labile toxin from enterotoxigenic Escherichia coli causes systemic impairment in Zebrafish modelpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/toxins13060419pt_BR
dc.identifier.urlhttps://doi.org/10.3390/toxins13060419pt_BR
dc.identifier.citationvolume13pt_BR
dc.identifier.citationissue6pt_BR
dc.subject.keywordheat-labile toxinpt_BR
dc.subject.keywordCaco-2 cellspt_BR
dc.subject.keywordzebrafishpt_BR
dc.subject.keywordsystemic effectspt_BR
dc.subject.keywordcardiotoxic effectpt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 13, n. 6, 419, jun. 2021pt_BR
dc.identifier.citationvancouver2021 June;13(6):419pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/17213-8pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/17213-8pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
item.grantfulltextopen-
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