Bothrops moojeni venom and its components strongly affect Osteoclasts’ maturation and protein patterns

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dc.contributor(LG) Laboratório de Genéticapt_BR
dc.contributorPrograma de Pós-Graduação em Ciências – Toxinologia (PPGTox)pt_BR
dc.contributorCENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributorLab. Bioquímicapt_BR
dc.contributor(LDI) Laboratório de Desenvolvimento e Inovação Industrialpt_BR
dc.contributor.authorD’Amélio, Fernandapt_BR
dc.contributor.authorVigerelli, Hugopt_BR
dc.contributor.authorPrieto da Silva, Álvaro Rossan de Brandãopt_BR
dc.contributor.authorFrare, Eduardo Osóriopt_BR
dc.contributor.authorBatista, Isabel de Fátima Correiapt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorKerkis, Irinapt_BR
dc.date.accessioned2021-07-16T17:10:56Z-
dc.date.available2021-07-16T17:10:56Z-
dc.date.issued2021pt_BR
dc.identifier.citationD’Amélio F, Vigerelli H, Prieto da Silva ARB, Frare EO, Batista IFC, Pimenta DC, et al. Bothrops moojeni venom and its components strongly affect Osteoclasts’ maturation and protein patterns. Toxins. 2021 June;13(7):459. doi:10.3390/toxins13070459.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3886-
dc.description.abstractOsteoclasts (OCs) are important for bone maintenance, calcium balance, and tissue regeneration regulation and are involved in different inflammatory diseases. Our study aimed to evaluate the effect of Bothrops moojeni’s venom and its low and high molecular mass (HMM and LMM) fractions on human peripheral blood mononuclear cell (PBMC)-derived OCs’ in vitro differentiation. Bothrops moojeni, a Brazilian lanced-head viper, presents a rich but not well-explored, venom composition. This venom is a potent inducer of inflammation, which can be used as a tool to investigate the inflammatory process. Human PBMCs were isolated and induced to OC differentiation following routine protocol. On the fourth day of differentiation, the venom was added at different concentrations (5, 0.5, and 0.05 µg/mL). We observed a significant reduction of TRAP+ (tartrate-resistant acid phosphatase) OCs at the concentration of 5 µg/mL. We evaluated the F-actin-rich OCs structure’s integrity; disruption of its integrity reflects bone adsorption capacity. F-actin rings phalloidin staining demonstrated that venom provoked their disruption in treated OCs. HMM, fraction reduces TRAP+ OCs at a concentration of 5 µg/mL and LMM fraction at 1 µg/mL, respectively. Our results indicate morphological changes that the venom induced cause in OCs. We analyzed the pattern of soluble proteins found in the conditioned cell culture medium OCs treated with venom and its fractions using mass spectrometry (LC-MS/IT-Tof). The proteomic analyses indicate the possible pathways and molecular mechanisms involved in OC reduction after the treatment.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(GSK) GlaxoSmithKlinept_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent459pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleBothrops moojeni venom and its components strongly affect Osteoclasts’ maturation and protein patternspt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/toxins13070459pt_BR
dc.identifier.urlhttps://doi.org/10.3390/toxins13070459pt_BR
dc.identifier.citationvolume13pt_BR
dc.identifier.citationissue7pt_BR
dc.subject.keywordosteoclastspt_BR
dc.subject.keyworddifferentiation cellularpt_BR
dc.subject.keywordtoxinspt_BR
dc.subject.keywordBothrops moojenipt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 13, n. 7, 459, jun. 2021pt_BR
dc.identifier.citationvancouver2021 June;13(7):459pt_BR
dc.contributor.butantanD’Amélio, Fernanda|:Aluno Doutorado|:Programa de Pós-Graduação em Ciências – Toxinologia (PPGTox):CENTD - Centro de Excelência para Descoberta de Alvos Moleculares|:PrimeiroAutorpt_BR
dc.contributor.butantanVigerelli, Hugo|:Pós-Doc|:Lab. Genética:CENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributor.butantanPrieto da Silva, Álvaro Rossan de Brandão|:Pesquisador|:Laboratório de Genéticapt_BR
dc.contributor.butantanFrare, Eduardo Osório|:Técnico|:Laboratório de Genética:CENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributor.butantanBatista, Isabel de Fátima Correia|:Pesquisador|:Laboratório de Biologia Molecular:CENTD - Centro de Excelência para Descoberta de Alvos Molecularespt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bioquímicapt_BR
dc.contributor.butantanKerkis, Irina|:Pesquisador:Docente permanente PPGTOX|:Laboratório de Genética:CENTD - Centro de Excelência para Descoberta de Alvos Moleculares|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/50040-4pt_BR
dc.sponsorship.butantanGlaxoSmithKline (GSK)¦¦pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦301974/2019-5pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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