
Bothrops moojeni venom and its components strongly affect Osteoclasts’ maturation and protein patterns
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor | (LG) Lab. Genética | pt_BR |
dc.contributor | Programa de Pós-Graduação em Ciências – Toxinologia (PPGTox) | pt_BR |
dc.contributor | (CENTD) Centro de Excelência para Descoberta de Alvos Moleculares | pt_BR |
dc.contributor | Lab. Bioquímica | pt_BR |
dc.contributor | (LDI) Lab. Desenvolvimento e Inovação Industrial | pt_BR |
dc.contributor.author | D’Amélio, Fernanda | pt_BR |
dc.contributor.author | Vigerelli, Hugo | pt_BR |
dc.contributor.author | Prieto da Silva, Álvaro Rossan de Brandão | pt_BR |
dc.contributor.author | Frare, Eduardo Osório | pt_BR |
dc.contributor.author | Batista, Isabel de Fátima Correia | pt_BR |
dc.contributor.author | Pimenta, Daniel Carvalho | pt_BR |
dc.contributor.author | Kerkis, Irina | pt_BR |
dc.date.accessioned | 2021-07-16T17:10:56Z | - |
dc.date.available | 2021-07-16T17:10:56Z | - |
dc.date.issued | 2021 | pt_BR |
dc.identifier.citation | D’Amélio F, Vigerelli H, Prieto da Silva ARB, Frare EO, Batista IFC, Pimenta DC, et al. Bothrops moojeni venom and its components strongly affect Osteoclasts’ maturation and protein patterns. Toxins. 2021 June;13(7):459. doi:10.3390/toxins13070459. | pt_BR |
dc.identifier.uri | https://repositorio.butantan.gov.br/handle/butantan/3886 | - |
dc.description.abstract | Osteoclasts (OCs) are important for bone maintenance, calcium balance, and tissue regeneration regulation and are involved in different inflammatory diseases. Our study aimed to evaluate the effect of Bothrops moojeni’s venom and its low and high molecular mass (HMM and LMM) fractions on human peripheral blood mononuclear cell (PBMC)-derived OCs’ in vitro differentiation. Bothrops moojeni, a Brazilian lanced-head viper, presents a rich but not well-explored, venom composition. This venom is a potent inducer of inflammation, which can be used as a tool to investigate the inflammatory process. Human PBMCs were isolated and induced to OC differentiation following routine protocol. On the fourth day of differentiation, the venom was added at different concentrations (5, 0.5, and 0.05 µg/mL). We observed a significant reduction of TRAP+ (tartrate-resistant acid phosphatase) OCs at the concentration of 5 µg/mL. We evaluated the F-actin-rich OCs structure’s integrity; disruption of its integrity reflects bone adsorption capacity. F-actin rings phalloidin staining demonstrated that venom provoked their disruption in treated OCs. HMM, fraction reduces TRAP+ OCs at a concentration of 5 µg/mL and LMM fraction at 1 µg/mL, respectively. Our results indicate morphological changes that the venom induced cause in OCs. We analyzed the pattern of soluble proteins found in the conditioned cell culture medium OCs treated with venom and its fractions using mass spectrometry (LC-MS/IT-Tof). The proteomic analyses indicate the possible pathways and molecular mechanisms involved in OC reduction after the treatment. | pt_BR |
dc.description.sponsorship | (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo | pt_BR |
dc.description.sponsorship | (GSK) GlaxoSmithKline | pt_BR |
dc.description.sponsorship | (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico | pt_BR |
dc.format.extent | 459 | pt_BR |
dc.language.iso | English | pt_BR |
dc.relation.ispartof | Toxins | pt_BR |
dc.rights | Open access | pt_BR |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | pt_BR |
dc.title | Bothrops moojeni venom and its components strongly affect Osteoclasts’ maturation and protein patterns | pt_BR |
dc.type | Article | pt_BR |
dc.rights.license | CC BY | pt_BR |
dc.identifier.doi | 10.3390/toxins13070459 | pt_BR |
dc.identifier.url | https://doi.org/10.3390/toxins13070459 | pt_BR |
dc.identifier.citationvolume | 13 | pt_BR |
dc.identifier.citationissue | 7 | pt_BR |
dc.subject.keyword | osteoclasts | pt_BR |
dc.subject.keyword | differentiation cellular | pt_BR |
dc.subject.keyword | toxins | pt_BR |
dc.subject.keyword | Bothrops moojeni | pt_BR |
dc.relation.ispartofabbreviated | Toxins | pt_BR |
dc.identifier.citationabnt | v. 13, n. 7, 459, jun. 2021 | pt_BR |
dc.identifier.citationvancouver | 2021 June;13(7):459 | pt_BR |
dc.contributor.butantan | D’Amélio, Fernanda|:Doutorado|:Programa de Pós-Graduação em Ciências – Toxinologia (PPGTox)|:(CENTD) Centro de Excelência para Descoberta de Alvos Moleculares|:PrimeiroAutor | pt_BR |
dc.contributor.butantan | Vigerelli, Hugo|:Pós-Doc|:Lab. Genética|:(CENTD) Centro de Excelência para Descoberta de Alvos Moleculares | pt_BR |
dc.contributor.butantan | Prieto da Silva, Álvaro Rossan de Brandão|:Pesquisador|:(LG) Lab. Genética | pt_BR |
dc.contributor.butantan | Frare, Eduardo Osório|:Técnico|:(LG) Lab. Genética:(CENTD) Centro de Excelência para Descoberta de Alvos Moleculares | pt_BR |
dc.contributor.butantan | Batista, Isabel de Fátima Correia|:Pesquisador|:Laboratório de Biologia Molecular:(CENTD) Centro de Excelência para Descoberta de Alvos Moleculares | pt_BR |
dc.contributor.butantan | Pimenta, Daniel Carvalho|:Pesquisador|:Docente Permanente PPGTOX|:Lab. Bioquímica | pt_BR |
dc.contributor.butantan | Kerkis, Irina|:Pesquisador|:Docente permanente PPGTOX|:(LG) Lab. Genética:(CENTD) Centro de Excelência para Descoberta de Alvos Moleculares|:Autor de correspondência | pt_BR |
dc.sponsorship.butantan | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/50040-4 | pt_BR |
dc.sponsorship.butantan | GlaxoSmithKline (GSK)¦¦ | pt_BR |
dc.sponsorship.butantan | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦301974/2019-5 | pt_BR |
dc.identifier.bvscc | BR78.1 | pt_BR |
dc.identifier.bvsdb | IBProd | pt_BR |
dc.description.dbindexed | Yes | pt_BR |
item.openairetype | Article | - |
item.languageiso639-1 | English | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
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crisitem.author.orcid | 0000-0002-8197-2260 | - |
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